Delighted to be at the Academic Neuroscience Centre Denmark Hill Campus to give a seminar for King's Health Partners @kingshealth @dev_neuro

CDN Xmas Party 2024🌲🐉🔥@dev_neuro

Xmas dinner 2024 Berninger Lab 🌲🥂🎁

RT @Rico_lab: Are you interested in the development of the human brain? One postdoctoral position is opened with #WT funds in @RicoLab to i….

RT @NeuroscienceNew: Reprogrammed Brain Cells Could Restore Damaged Circuits. New research transforms brain support cells into functional n….

RT @NeuroscienceNew: Reprogrammed Brain Cells Could Restore Damaged Circuits. Researchers have successfully reprogrammed brain support cell….

RT @GamarnikLab: Scienticide’: Argentina’s science workforce shrinks with Milei government .The main science agency, the National Council o….

Enjoying beautiful Braga 🇵🇹 Thanks to Minho Medical Meeting for the invitation to present our work🧠

RT @neuroliege: Reprogramming astroglia into neurons with hallmarks of fast-spiking parvalbumin-positive interneurons by phospho-site–defic….

RT @ChristopheHein2: Very nice study showing in vivo reprogramming of cortical astrocytes into fast spiking interneurons !.

Honour and pleasure to share with amazing and talented team with Sophie Péron and Ana Beltran. And thanks to all co-author! and Berninger Lab at @dev_neuro.

Reprogramming glia into neurons is challenging. Although our approach is unlikely to generate bona fide FS/PV+ interneurons, our findings may thus pave the way towards leveraging lineage reprogramming of glia into this important subtype of interneurons as a new therapeutic avenue.

Finally, we found that FS and PV-expressing iNs can be generated in an ectopic location in the cortex (Layer I), suggesting that TFs can exert a determinant effect on the expression of interneuron subclass hallmarks irrespective of influences from local environment

A high proportion of Ascl1SA6/Bcl2- iNs were capable of high-frequency action potential firing, a hallmark of fast-spiking interneurons.

Our greatest surprise came with the observation that many of the neurons induced by Ascl1SA6 and Bcl2 exhibited hallmarks of a particular subtype of cortical interneurons: they expressed parvalbumin and.

Rigorous genetic fate mapping clearly revealed the astroglial origin of most of the induced neurons, while oligodendrocyte progenitor cells contribute only marginally. This result again highlights the importance of cellular context for glia-to-neuron conversion.

We took advantage of the specificity of retroviruses for selectively targeting cells in mitosis in the early postnatal cortex and found that overexpression of Ascl1SA6 in combination with Bcl2 efficiently converts postnatal cortical glia into neurons.

Here we wondered whether utilizing a more neurogenic phospho-deficiet-mutant Ascl1 (Ascl1SA6) could enhance glia-to-neuron conversion in vivo.

Ascl1 has been used for converting somatic cells into neurons. However, it neurogenic activity is highly cell-and context-dependent. Asc1 failed to convert glia in the early postnatal cortex into neurons and instead promoted the proliferation of OPCs.

Our paper on glia-to-neuron conversion is out in @ScienceAdvances. We generated neurons with the capacity of true high-frequency action potential firing and expressing parvalbumin from early cortical astrocytes in vivo💥👇🏻🧵