Thrilled to share that our new preprint is out on @biorxivpreprint! Enhanced RNA-targeting CRISPR-Cas technology in zebrafish performed at the Moreno-Mateos lab @crisprscan @CABD_UPO_CSIC. A thread (1/13):

¡Enhorabuena al equipo responsable por el trabajo publicado en @embojournal que ha sido co-liderado por @BazziniLab de @ScienceStowers & @crisprscan del #CABD!!!

Fenomenal nota de prensa de nuestra última contribución para entender un poco mejor los primeros eventos durante la embriogenesis. @Luisherhuertas @CABD_UPO_CSIC @bazzi

Nota de prensa sobre nuestro último artículo publicado en @embojournal 🎉 Una fantástica colaboración entre @crisprscan y @BazziniLab

Very proud to have participated in this amazing work published in @embojournal where we have described Bckdk as a post-translational regulator of MZT in teleosts 🐟 using our optimized CRISPR-RfxCas13d system. Congrats @luisherhuertas, @crisprscan and all participants!! 🎉👏🏽

Still open to get applications for this postdoctoral position. Please send me the required information. Also, I will be in two weeks in Sorrento for the CRISPR-Cas workshop, so if you are around and would like to work in our lab, this would be a great opportunity to meet :)

El contrato para realizar tu tesis doctoral en inestabilidad genetica y procesos tumorales en Anemia de Fanconi ya esta disponible. Solicitala!! Please RT! https://t.co/kXxcykO1Bj

Glad to share that the website for the MZT EMBO workshop that I organize together with Ana Boskovic (EMBL, Rome), Antonio Giraldez (Yale U) and Melissa Harrison (U Wisconsin) is out: https://t.co/ewGlf67Yg8 Please spread the word and join us in beautiful Seville next 4-7 May!

Want to now more about our last paper in @NatureComms? 🚀 We expand & consolidate the RNA-targeting #CRISPRCas toolbox 🔬🧬 Read more in @SpringerNature communities 👇 https://t.co/5X7HE6w0nw @crisprscan @CABD_UPO_CSIC @Luisherhuertas @nahon_daniel #CRISPR #RNA #Biotechnology

Si estás interesa en hacer una tesis doctoral en tumorogenesis asociada a la inestabilidad genética, contáctanos!!! Please RT!!

Muy contento de haber liderado este trabajo junto con dos magníficos compañeros @Luisherhuertas @nahon_daniel en el Moreno-Mateos lab @crisprscan @CABD_UPO_CSIC. ¡Gracias a todos los compañeros y colaboradores por el gran trabajo!

El equipo del Centro Andaluz de Biología del Desarrollo @CABD_UPO_CSIC ha conseguido optimizar diferentes sistemas CRISPR-Cas in vivo usando el pez cebra como modelo https://t.co/PUrMX7ytB4

First seen in Biorxiv and now published and updated with more data in @NatureComms. With this work we have further enhanced CRISPR-Cas RNA targeting in vivo using transient approaches such as RNP or mRNA-gRNA in zebrafish embryos as vertebrate model.

Our last work is now in @biorxivpreprint. Great work of @gopalkushawah in collaboration with @BMRL_Danielson @huzaifahassan @mmarchin and @S_Nowotarski all done at @ScienceStowers @StowersGrad https://t.co/w0kKXtoTOR

It was a fantastic lab retreat with a lot fun, new ideas and great scientific discussions. Super proud of this excellent team we have built since 2019! And thanks to our invited colleagues @BalbontinLab @RosadoLab and Thirsa Brethouwer for their invaluable contribution!

And, of course, thanks to the institutions that fund our lab! @pablodeolavide, @CSIC, @CienciaGob, @UniversidadAnd, @PpccSalud and @Synthego that awarded us with the Genome Engineer Innovation Grant some years ago (13/13)

@AdMOSC_Research from @IIQ_US_CSIC, A. J. Treichel, @GaPescador and @gopalkushawah from @BazziniLab @ScienceStowers, @JnGiS and @NietoAngela from @NeuroAlc, @KevinHoldenSF and J.A. Walker II from @Synthego (12/13)

This work has been performed together with outstanding PhD students @Luisherhuertas and @nahon_daniel with the contribution of Carlos Gómez-Marín, Manuel J. Muñoz, @proteolaura, @pmmargar and @AleCanoRuiz from @CABD_UPO_CSIC (11/13)

Overall, our work constitutes a significant contribution towards better comprehension and enhancement of transient CRISPR-Cas approaches to target RNAs facilitating a more effective integration into in vivo KD-based biotechnological and biomedical applications (10/13)

Fifth, to complete the RNA-targeting CRISPR-Cas toolbox in zebrafish, we revealed that Cas7-11 and DjCas13d can actively eliminate highly abundant and ectopic RNAs (GFP) with absent or lower collateral effects (dsRed levels), being DjCas13d equally efficient than RfxCas13d (9/13)

Forth, we demonstrated that collateral activity is triggered when transient CRISPR-RfxCas13d approaches target extremely abundant and ectopic mRNAs such as reporters, inducing non-targeted DsRed fluorescence decay, 28S rRNA fragmentation and developmental defects (8/13)