Gabriel da Silva Pescador
@GaPescador
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Developmental biologist interested in protein regulation during cell-fate transitions @BazziniLab @ScienceStowers 🇧🇷
Joined April 2019
(1/7) I am excited to share that my paper is out @CellReports ! I am thankful to @BMRL_Danielson , @BazziniLab , @StowersGrad and the proteomics core @ScienceStowers for all the support.
Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis
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Great collaboration with @crisprscan Enhances RNA-targeting CRISPR-Cas technology in zebrafish. 2024. https://t.co/BoORMDTjYw Congratulations @IsmaelMSanchez @Luisherhuertas @GaPescador @gopalkushawah @StowersGrad @ScienceStowers @CABD_UPO_CSIC
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(7/7) We hope the generated datasets and analysis will allow the field to help define the best approach to unravel gene function, be it CRISPR-Cas9 for knockouts, CRISPR-Cas13d for mRNA knockdown or, degron systems to directly target proteins for degradation.
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(6/7) Last, but not least, we utilized the CRISPR-Cas13d system as a proof of concept to knock-down znf281b, a gene that is rapidly translated before genome activation, and we identified a critical role of znf281b in early zebrafish development.
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(5/7) While genes have been extensively classified as maternal or zygotic based on their RNA expression, we were surprised to find zygotic genes deposited as protein since fertilization in both zebrafish and mice, challenging the definition of zygotic genes.
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(4/7) We found that the massive RNA changes at these stages is not reflected in changes to protein levels, with many genes only changing at either the RNA or protein level, and only a subset changing at both regulatory layers, including some with divergent RNA vs protein effects.
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@CellReports @BMRL_Danielson @BazziniLab @StowersGrad @ScienceStowers (3/7) In our paper, we performed quantitative proteomics, integrating transcriptomics and translatomics in the first 6 hrs of zebrafish embryogenesis to correlate protein and RNA regulation. Check out our ShinyApp to view expression of your favorite gene:
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@CellReports @BMRL_Danielson @BazziniLab @StowersGrad @ScienceStowers (2/7) While the field has extensively studied RNA regulation during the maternal-to-zygotic transition, we were interested in how proteins are regulated in comparison to RNAs during this crucial developmental process.
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Excited to share my work today at #2024SDB ! If you are interested in protein regulation during maternal-to-zygotic transition, or if you want to learn how CRISPR-Cas13d allowed us to study a new genome activator come chat! @BazziniLab @ScienceStowers @StowersGrad @SocDevBio
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It was a great conference! So nice to see the shared mentor-mentee talks. Shout out to @BazziniLab @kkostova1 @ScienceStowers for organizing it! #SRCKC24
Last week the Institute held #SRCKC24, an intimate & innovative conference that allows PIs to share the stage with trainees. Listen to what they had to say while attending and stayed tuned for information on the next SRC coming soon! #RNA #Biology MORE: https://t.co/3zKjMoFSDS
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Happy to share our new paper: "miR-430 regulates zygotic mRNA during zebrafish embryogenesis" published online in Genome Biology @GenomeBiology . Congratulation @BMRL_Danielson
@ScienceStowers @StowersGrad Paper👇👇
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It’s finally out! 👯🎉🎊 Take a look at our paper on the role of microRNAs on zygotic transcripts during early vertebrate development: @BazziniLab @ScienceStowers
https://t.co/tUYBlTbXvP
genomebiology.biomedcentral.com
Background Early embryonic developmental programs are guided by the coordinated interplay between maternally inherited and zygotically manufactured RNAs and proteins. Although these processes happen...
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The @BazziniLab brought some cheer & gifts to so many of the people @ScienceStowers that help us throughout the year! Lots of fun and I hope our reindeer put a smile on a lot of faces 🦌🎁🎄 Happy Holidays!!!
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All #SRCKC23 speakers are invited to present tandem-talks with early career researchers from their labs. Join us Oct 11-13 as @WickstromLab & @Clem_Villeneuve co-present "Coordination of cell states and morphogenesis by mechanical force" @ScienceStowers
https://t.co/nJHeDleVor
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The videos from MaxQuant Summer School 2023 in Boston are up on our YouTube channel. Here is the playlist in case you want to watch them: https://t.co/nWOyyiY7CE
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#Undergraduates #Postbacs Back by popular demand! @SocDevBio is hosting a webinar Get Into Grad School August 30 at 2pm ET. Learn about doctoral programs, the key elements of a compelling grad school application, and the interview process. Register: https://t.co/YeZ85zVEe2
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What's interesting about caves? #Cavefish! #StowersGrad predoc, Fanning Xia (@Xiazi_starry), presented at #SDB2023. 👏 #PhD #GradSchool @SocDevBio
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Proud of my mentee presenting her summer work today! 😊
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Interested in protein regulation during early embryogenesis? Or want to check the unexpected things we found when correlating protein and RNA during maternal-to-zygotic transition? Drop by my poster today and let’s chat! #2023SDB
Excited to be in Chicago for the 82nd SDB meeting! Come to my poster if you are interested in genome activation and maternal-to-zygotic transition seen at the protein level! @SocDevBio @BazziniLab @ScienceStowers
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