Green Lab
@GreenLabJHMI
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Ribosome is life. Tweets by grads/postdocs, approved (sometimes) by Rachel Green
Joined October 2017
Double whammy! 🧬 Our latest collaboration with the Zaher/Beckmann/Inada labs reveals how Mbf1/EDF1 senses ribosome collisions to promote Gcn2 activation, allowing optimal induction of eIF2α phosphorylation and de-repression of GCN4 translation.
cell.com
Mbf1 was proposed to function as a transcriptional coactivator during induction of the ISR, bridging a physical connection between Gcn4 and the transcription machinery. Kim et al. challenge this,...
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Check out our paper in this month's issue of RNA! We found that the translation elongation inhibitor emetine stabilizes hundreds of mRNAs many of which encode C2H2 zinc fingers!.
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Excited to see our LARP1 manuscript published at The EMBO Journal. We show that LARP1 directly binds non-translating ribosomes and TOP mRNAs. An excellent collaboration with the @jingdongcheng lab!.
embopress.org
imageimageThe LARP1 protein regulates translation and stability of mRNAs characterized by a terminal oligopyrimidine (TOP) motif, which encode most proteins of the translation machinery. This study...
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We’ve captured the splitting image of Slh1: our work on an enzyme that splits collided ribosomes in E. coli is out on bioRxiv! Huge thanks to the @BeckmannLab, Burroughs, and Aravind for a great collaboration.
biorxiv.org
Although many antibiotics inhibit bacterial ribosomes, loss of known factors that rescue stalled ribosomes does not lead to robust antibiotic sensitivity in E. coli , suggesting the existence of...
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ZAK triggers both cell survival and death pathways in response to ribotoxic stress. We investigated how the choice between these pathways is made and identified feedback mechanisms that regulate ZAK activity, determining whether a cell survives or dies.
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We’re excited to share our study in @CellCellPress on the role of the MAP3 kinase ZAK in UV-induced apoptosis led by @2primehydroxyl! We studied cell signaling kinetics following UV-damage and found that ZAK drives apoptosis via ribosome collisions.
cell.com
A comprehensive analysis of molecular and cellular changes following UV irradiation reveals that the ribotoxic stress response, rather than the DNA damage response, mediates UV-dependent programmed...
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It’s official! MutS2 still doesn’t cut it. Congrats @Esth3rPark and @BeckmannLab!.
embopress.org
imageimageRibosome stalling in the middle of a translated mRNA leads to collisions and triggers quality control pathways. Using structural and biochemical approaches, this study reveals how MutS2...
Turns out MutS2 just doesn’t cut it ✂️ despite it having an SMR domain! Our latest collaboration with the @BeckmannLab characterizing the role of MutS2 in splitting stalled ribosomes in B. subtilis.
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Check out our new preprint in collaboration with @jingdongcheng lab about how LARP1 senses the abundance of non-translating ribosomes to help cells decide whether or not to make ribosomal proteins!.
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RT @DrJoshBlack: I’m excited to share our News & Views article that @GreenLabJHMI and I wrote for @NatureCellBio on an exciting study from….
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RT @DjuranovicLab: It was great few days in Baltimore to celebrate 25 years of @GreenLabJHMI and see Rachel as well as almost all former me….
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RT @binwu1: Our work "Bursting translation on single mRNAs in live cells" is now published on Molecular Cell. It….
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The Green lab is looking to hire a postdoc to study ribosome stalling and rescue in bacteria! See our official posting (link below) for details.
greenlabjhmi.org
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Undergrad no more - congrats to Rebekah for her graduation and promotion to lab tech!
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Turns out MutS2 just doesn’t cut it ✂️ despite it having an SMR domain! Our latest collaboration with the @BeckmannLab characterizing the role of MutS2 in splitting stalled ribosomes in B. subtilis.
biorxiv.org
Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli , these pathways are triggered by ribosome collisions through...
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RT @DjuranovicLab: I am thankful to all my students, postdocs and lab mates, my mentors (@GreenLabJHMI, @mpitoolkit, Vukic Soskic) as well….
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RT @binwu1: Excited to share our preprint about "Bursting Translation on Single mRNAs in Live Cells" by Nathan Livi….
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