Our RAPPL purification of ribosomes and associated material is finally out. I am really proud of this study in collaboration primary with @slavicaPD lab, as well as Marko Jovanovic and @Hashemyaser4 Labs. This method aims to replace 60 years old purification of ribosomes by

Four days of science at @embl in Heidelberg for "Protein Synthesis and Translational Control" meeting.

Thanks to @KurianLab for hosting me today at Goethe University at Frankfurt. It was great to discuss science with everyone at Frankfurt.

We are happy to announce that we are kicking off with NCI RNAMoDO U01 grant together with Gregory, Alfonzo and Piskounova Labs. This will be a great use of RAPPL and RAPPL-Ribo-Seq in RNA driven research on cancer.

One use of RAPPL that I forgot to comment on is tandem purifications of nascent polypeptide chain or initiation factors if you are interested in protein folding, chaperons or translation initiation. More of this to come. initiation focus on attached image.

RT @ImmaginaBiotech: 🌍 Our RNA Symposium is bringing top RNA experts from academia & industry this October! Don’t miss the chance to join i….

or one can in an hour get material for cryoEM analyses of ribosome complexes with atomic resolution. More on use of RAPPL still to come.

Ribosomes isolated like this can be used for functional assays ie. drug resistance in bacteria

The complexes stay intact for studies of translation inhibitors or nascent polypeptide chains in ribosomes

This method not only saves time, but is also economical, more efficient and mild in isolation conditions, preserving translation complexes, ribosome associated proteins and enables isolation of ribosomes for structural (cryo-EM) and functional studies. Ribosomes and associated

Our collaboration with @TonyMustoe lab is out today. MPRA libraries and possible cryptic splicing in MPRA reporters, or reporters in general. The additional novelty here is that such events are controlled or influenced by U-rich motifs. Soon more on this topic.

First RAPPL ribosome isolation at the new lab space at Brown University. Purification from a small culture of parasitic Eukaryote. Soon out.

@DjuranovicLab is from today officially part of @BrownUniversity and Brown RNA center. We will have our new website soon.

New structure of protist ribosome using RAPPL.

Another great collaboration with Topisirovic Lab at McGill Universoty came out today. This is also use of our first patent. In @elife: Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics

Last month @uspto issued yet another patent for @slavicaPD @DjuranovicLab and Jessey Erath for use of polybasic peptide or non-peptide moieties against malaria parasites. More soon.

another eukaryotic and archaeal ribosome done by RAPPL

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months . 2 bacterial and 2 eukaryotic in this case (1/2).