The Bustamante Lab
@BustamanteLab
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Prof. Carlos J. Bustamante's Single-molecule Biophysics Laboratory at UC Berkeley
Berkeley, CA
Joined July 2019
Excited to share our new paper in @MolecularCell! .Congratulation to the transcription team @Franburgos_ AlexTong, ChenLi, @CesarDiazC and our fantastic collaborators @ReinbergDanny & Kaplan labs! @BustamanteLab . #Transcription #Chromatin #SingleMolecule #OpticalTweezers #FACT.
Online Now: FACT weakens the nucleosomal barrier to transcription and preserves its integrity by forming a hexasome-like intermediate
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Check out our recent article in collaboration with the Fan lab:.@BustamanteLab work led by @Honglu_Zhang in @ScienceAdvances!.Congrats to all authors involved! @cccDNA.
science.org
DNA origami force probes and AlphaFold combine experiments and AI to decode the dynamics of protein folding.
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Our last nucleosome work in collaboration with the Ren group: Nucleosome arrays visualized by individual particle cryo-ET. Congratulations @Meng3054 , @CesarDiazC , Jianfang Liu, Jinhui Tao, Paul Ashby, Carlos , and Gary Ren!!.
nature.com
Nature Communications - Here, using cryo-ET, the 3D structures of individual nucleosome particles were characterized to observe changes under varying ionic strengths and in the presence of protein...
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Our last work on nucleosome disassembly using High-speed AFM. Can we visualize the nucleosome plasticity using AFM imaging? Congratulations to Bibiana Onoa and the nucleosome team!! @cccDNA @CesarDiazC and Antony Lee.
Witnessing the unraveling of chromatosomes and nucleosomes in real-time with AFM! The intricate stepwise disassembly reveals a mechanism for unlocking DNA for vital cellular processes. #DNAUnlock#AFM. NEW #ASAP.Read it here:
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RT @Henry_de_belly: I am beyond excited that our story where we address the modalities of membrane tension propagation in cells is out @Cel….
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RT @UCB_Chemistry: Very!!! exciting news from the labs of Profs. Ting Xu, Carlos Bustamante and Haiyan Huang @TingXu_Berkeley @BustamanteLa….
news.berkeley.edu
Using AI, Ting Xu and her colleagues designed polymer mixtures that mimic the natural proteins in biological fluids. The technique could improve the design of biocompatible materials.
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RT @NogalesLab: Check out this this wonderful collaboration with @BustamanteLab . Congratulations to our biophysics PhD student @AlfredoFl….
cell.com
A combination of bulk, single-molecule, high-throughput sequencing, and cryo-EM demonstrates that the ribosome, through the action of mechanical force and allostery, enhances the activity and reduces...
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RT @UWBiochem: This semester's Steenbock Lectures will be given by Carlos Bustamante @UCBerkeley. Bustamante's lab focuses on single molecu….
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RT @Henry_de_belly: Really hyped to share this preprint of some of my postdoctoral work with @OWeinerLab @virtual_embryo @BustamanteLab in….
biorxiv.org
Membrane tension is thought to be a long-range integrator of cell physiology. This role necessitates effective tension transmission across the cell. However, the field remains strongly divided as to...
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New Review! “The Development of Single Molecule Force Spectroscopy: From Polymers Biophysics to Molecular Machines” #Biophysics #SingleMolecule.
The Development of #Single_Molecule_Force_Spectroscopy: From Polymer Biophysics to #Molecular_Machines. @BustamanteLab.
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Hola, our latest work is now up on PNAS. Mechanical disassembly of nucleosomes using optical tweezers with fluorescence detection (Fleezers). @CesarDiazC, @cccDNA, Robert, Juan Pablo, @Meng3054, Enze, @AndyQuaen, Michael, Ryan, Bibiana, and Carlos.
pnas.org
Nucleosome DNA unwrapping and its disassembly into hexasomes and tetrasomes is necessary for genomic access and plays an important role in transcri...
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Hola, the final version of our latest paper is now up on Mol Cell. The question: how does nucleosome arrays form liquid droplets? By a two-step process. @Meng3054, @CesarDiazC, Bibiana, @cccDNA, @katy_requejo, Jianfang, Michael, Eva, Gary and Carlos.
cell.com
Zhang et al. demonstrate that liquid-liquid phase separation of tetranucleosome arrays occurs in two steps: a spinodal decomposition involving formation of loosely arranged irregular condensates,...
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Congratulations @kingcada!!.
Ever stop and wonder how proteins cut membranes?. It turns out this is actually a complicated question. There are many proteins that employ different mechanisms but for today, I'd like to focus on a set of proteins called ESCRTs. (
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Hola everybody, we are excited to share our last preprint about nucleosome disassembly under force using optical tweezers combined with fluorescence detection. @CesarDiazC , @cccDNA , Robert, Juan Pablo. #nucleosome #single-molecule #opticaltweezers.
biorxiv.org
Nucleosome DNA unwrapping and its disassembly into hexasomes and tetrasomes is necessary for genomic access and plays an important role in transcription regulation. Previous single-molecule mechani...
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RT @SOBLA_BIOF: El Viernes 3 de Diciembre Juan P. Castillo, @BustamanteLab, va a presentar en el Journal Club de la SoBLA su artículo en @N….
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Dear community, we are excited to share our preprint about the 3D molecular architecture of nucleosome liquid droplets. Congrats to the authors of this work: @Meng3054, @CesarDiazC, Bibiana, @cccDNA, @katy_requejo, Michael, Eva, Gary and Carlos.
biorxiv.org
It has been proposed that the intrinsic property of nucleosome arrays to undergo liquid-liquid phase separation (LLPS) in vitro is responsible for chromatin domain organization in vivo . However,...
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RT @MarquseeLab: @zhijie_berkeley and Alan's collab w/ Quan Wang's lab is published in PNAS! Congrats to all authors! . "Single-molecule di….
pnas.org
Theoretical and experimental observations that catalysis enhances the diffusion of enzymes have generated exciting implications about nanoscale ene...
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RT @zha_lab: We will be welcoming three Rising Stars on Social DNAing this week! The future is calling. Sign up at .
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New Paper: A DNA packaging motor inchworms along one strand allowing it to adapt to alternative double-helical structures #RingATPases #molecularmotors #singlemolecule .
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New Review: Optical tweezers in single-molecule.biophysics @Nature #CarlosBustamante #SingleMolecule .
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