Marqusee Lab
@MarquseeLab
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The Marqusee Lab @UCBerkeley uses #biophysics to study #proteinfolding in basic and cellular contexts. Account is student/postdoc run. All tweets in kcal/mol.
Berkeley, CA
Joined May 2020
Check out Naomi's paper examining domain coupling in mGlu receptors on bioRxiv! A fun collaboration with the @IsacoffLab and also featuring great work from our undergraduate researcher Sam!
biorxiv.org
The G protein–coupled metabotropic glutamate receptors form homodimers and heterodimers with highly diverse responses to glutamate and varying physiological function. The molecular basis for this...
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Check it out and let us know what you think! We are really excited by the combination of HDX-MS with eVLPs as it provides an opportunity to more easily study viral fusion proteins and other membrane proteins in native-like environments with HDX-MS. 7/7.
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D614G shifts the equilibrium to favor the prefusion conformation while the S2' site stays disordered. This study provides, to our knowledge, the first molecular explanation for the dual evolutionary advantages of furin cleavage and D614G. 6/7.
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However, furin cleavage also increases the population of the open-interface trimer, potentially promoting premature S1 shedding. We propose that this trade-off drove the evolution of the D614G mutation, an early fixed mutation in SARS-CoV-2. 5/7.
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We find that furin cleavage at the S1/S2 site increases the disorder of the S2’ protease site, which may increase TMPRSS2 activity leading to more efficient fusion. This provides a mechanistic explanation for furin cleavage enhancing infectivity. 4/7.
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We show that the native, full-length spike samples the previously identified open-interface trimer conformation, and its populations and interconversion rates are sensitive to sequence variations. 3/7.
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We use eVLPs to display full-length SARS-CoV-2 spike, both with and without prefusion-stabilizing prolines, and then probe dynamics with HDX-MS. Our findings reveal significant changes in spike dynamics resulting from natural and engineered variants. 2/7.
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Check out Sophie's new preprint on bioRxiv in collaboration with Magnus Hoffmann's lab where we conduct HDX-MS on SARS-CoV-2 spike proteins displayed on eVLPs to understand the dynamics of natural and engineered variants. 1/7
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RT @antoinekoehl: Super cool work by twitterless Naomi and crew looking at domain coupling in mGlu receptors. @MarquseeLab @IsacoffLab . Do….
biorxiv.org
The G protein–coupled metabotropic glutamate receptors form homodimers and heterodimers with highly diverse responses to glutamate and varying physiological function. The molecular basis for this...
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RT @LindorffLarsen: Big congratulations to PhD student Thea K Schulze from our PRISM centre on being awarded the elite research travel gran….
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.@NRDall's paper is up on bioRxiv! Using high-throughput in vivo studies and biophysical in vitro assays (CD and HDX/MS), we show how circular permutation affects the expression of HaloTag in different cell environments, and how CP impacts protein stability and folding pathways.
The importance of the location of the N-terminus in successful protein folding in vivo and in vitro #biorxiv_biophys.
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RT @ScienceyRosalie: It’s out! Via hydrogen deuterium exchange, biochemistry, and cryo-EM, we (Walter lab, @MarquseeLab et al) discovered….
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RT @evan_s_obrien: Happy to be a part of such a cool story! Structural & dynamic characterization of mGluR intermediate states. Congratulat….
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Amir will present his work on cotranslational folding of the SARS CoV-2 RBD at poster board 15AB. Check out his abstract (#328) and give him a visit!.
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Susan and @amir_bitran are in Boston for the Protein Society! Catch Susan's plenary talk on Thursday morning, and check out Amir's poster on Saturday evening.
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See Charlotte's new manuscript on bioRxiv.
The importance of input sequence set to consensus-derived proteins and their relationship to reconstructed ancestral proteins #biorxiv_biophys.
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Congratulations to our grad student Darren Kahan on being awarded the @advlightsource Doctoral Fellowship in Residence! He will be working with Dr. Corie Ralston’s lab at the @molecularfndry.
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RT @biorxiv_biophys: Secondary Structure Detection Through Direct Nanopore RNA Sequencing #biorxiv_biophys.
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RT @UCBerkeley: Chemist Susan Marqusee takes leading role at National Science Foundation.
news.berkeley.edu
Marqusee, a biophysical chemist, lead the directorate for biological sciences at the NSF, which is responsible for about $1 billion in funding annually
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