Also take a look at complementary work by @SvejstrupLab: https://t.co/gRVSqcra2R

Thanks to support from @SingMolSci and @UNSWRNA

When both ARMC5 and INTS8 are depleted, excess Pol II is released into genes but often fails to transcribe the full transcription unit, resulting in major gene expression changes...lots more results and discussion in the paper, of course!

This finding pushed us to try to understand what stops Pol II from entering the elongation phase and actually making more RNA. We found that ARMC5 and Integrator phosphatase play complementary roles in this process.

ARMC5 also regulates homeostatic Pol II levels - seemingly responsible for around half the cell’s Pol II turnover. But the extra Pol II that accumulates in cells upon ARMC5 loss doesn’t lead to more Pol II in gene bodies: it is mostly in the free pool or in the proximal promoter.

However, using live-cell imaging and cellular fractionation, we found that this excess Pol II mostly accumulates off-chromatin.

It has long been known that Pol II is degraded when transcription is inhibited - either with small molecules or if specific cofactors are depleted. We now know the ubiquitin ligase responsible is CUL3-ARMC5. When we remove ARMC5, Pol II is no longer lost upon inhibition.

Building on our previous work on RNA concentration homeostasis https://t.co/3C6MNqe4Kz, we were interested in how global levels of Pol II are controlled. We teamed up with Ana's lab who delve deep into mechanisms of ubiquitin-mediated regulation of the transcription cycle.

📢 New paper from the lab! In a fantastic new collaboration with @ana_tufegdzic lab, we characterise a major Pol II homeostasis pathway in human cells. Congratulations Roberta @caciopporobi, Alex @acftbl, Ivan @IvanShlamovitz, and Andrew. https://t.co/B4xOaHDR7V

Was a pleasure working with @ana_tufegdzic on this!

Global transcription regulation revealed from dynamical correlations in time-resolved single-cell RNA sequencing @CellSystemsCP @DVolteras @VShahrezaei https://t.co/JgLDygkBC2

My group is looking for new PhD students! Australian and International applicants welcome. https://t.co/Vxd85ACIHF

Genome-wide quantification of RNA flow across subcellular compartments reveals determinants of the mammalian transcript life cycle https://t.co/sPoFmZu5Uk Published @MolecularCell Big thanks to fellow co-first authors @BrendanSmalec Albert Xu @stephenfloor mentor @fiddle +team!

We were selected for the cover! our papers under the spotlight in the current issue of @MolecularCell. Congratulations to Govind Menon for his beautiful design 😍☺️

Cofactors are a crucial part of the gene activation process. Yet, we still don't have a comprehensive understanding of how and why they are used by different genes. Proud to present our attempt to address this key question, now published https://t.co/wdDmHDMDCI

1/2 The @nswdpi pilot project to fast-track an mRNA vaccine for border disease virus (BDV) has been successfully manufactured for the first time in NSW by the @UNSWRNA.

We at the @UNSWRNA team are very proud of this that @dpinsw led! First batch of an animal mRNA vaccine that was made here in Australia by us in March in collaboration with @Tibabiotech who developed this vaccine. #mRNA

Australian PhD candidates & Early Career Scientists, don’t miss this opportunity to participate in a dedicated 2 day program in the lead up to Biomolecular Horizons 2024. Connect with colleagues from across the globe! Visit https://t.co/tcLNjq15Jk Applications close 29 Feb 2024

Very excited to have Wei Xie over in Melbourne in September as part of this great meeting!

Abstract submissions for @bmh2024Melb are now open! You can nominate the preferred theme and symposium relevant to your work. Click on below for more information. Submissions close on Tuesday 12 March 2024. https://t.co/mS9ApNyeRB