Lucien Hinderling
@lhinderling
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tries to make microscopes smarter · bioimage analysis, optogenetics, ml, 3d printing, open science · phd student in cellular signalling dynamics @Pertzl
Joined January 2021
Real-time segmentation of fibroblasts allows us to grow actin skeleton in a specified direction! Cells built @Pertzlab express optogenetic construct to activate RAC. We use @manager_micro and @napari_imaging to train a pixel classifier for segmentation on live data from scope. 🧵
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RT @ralph_ep: New preprint!. tl;dr — We ran around late at night to record wild rats in NYC and figured out how to quantify their behavior….
biorxiv.org
Urban rats are highly adaptable, thriving in the dynamic and often inhospitable conditions of modern cities. Despite substantial mitigation efforts, they remain an enduring presence in urban enviro...
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@PertzL next steps for us will be: decrease hardware cost, use image feedback for closed-loop control. many interesting projects on hacking cheap 3D printers out there already (see e.g.
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@PertzL interested to see what other applications people will find! what would you print? github repo:.
github.com
Use your microscope for microfabrication. Contribute to hinderling/fabscope development by creating an account on GitHub.
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@PertzL we use off-the-shelf 3D printing resin and microscope slides, replacing expensive SU-8 and silicon wafers. no clean-room required. see the preprint thread for application examples with focus on biology
FAST: from idea to prototype within a day. we create a simple protocol, combining ideas from literature and adding our own tricks. focus on replacing expensive and toxic chemicals: SU8 → consumer 3D printing resin, chloro silane → methanol, silicon wafers → glass slides.
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PAPER OUT ✨ "hack" your microscope to work as a 3D printer! micrometer sized features at cm scale. <5$ in material costs per chip. from idea to experiment within a day. first paper of my phd in PertzLab @PertzL to make it through peer review!
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RT @onenimesa: Have you ever thought about inflating tissues? .Or maybe quickly deflating those inflated tissues? . New #EpithelialMechan….
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RT @J_Cell_Sci: Joe Tyler @jtyler91, Anthony Davidson, Vassilis Koronakis & team find that inhibition of PAK activity leads to the producti….
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RT @eLife: One protein, two opposing roles: This ‘compelling’ study shows how the same signalling molecule can drive either protrusion or r….
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RT @eLife: One protein, two opposing roles: This ‘compelling’ study shows how the same signalling molecule can drive either protrusion or r….
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RT @Fr_BioImaging: Don't miss the first Smart Microscopy Days on YouTube! 🔬. 📅 June 16th & 17th.▶️ Hosted on RTmfm’s YouTube channel. Organ….
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RT @inscoper: Excited to join the first-ever Smart Microscopy Days, powered by IBDM & France-BioImaging!. Catch INSCOPER’s Rémy Torro live….
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Also fixes the issue with non-editable legends when exporting from matplotlib
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Inkscape plugin to rescale figures without distorting the text, and other useful features!
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RT @abzzram: Hi bio+ML folks! Check out our recent work modeling live-cell microscopy data with fixed-cell protein measurements. Here are….
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RT @abzzram: Are you using phospho-ERK staining to infer Ras/MAPK signaling history of fixed cells? . Check out our recent work demonstrati….
nature.com
Nature Communications - ERK signaling involves complex spatiotemporal dynamics, making it difficult to quantify in many systems. In this study, live-cell activity measurements are combined with...
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RT @paoloarmando83: Superb article from @abzzram and @albecklab demonstrating that single-cell ERK history can be reconstructed by end-poin….
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Can only recommend! Allows you to approach microfab like 3D printing - any design can just be prototyped within a couple hours. Game changer for our lab.
Also a DMD / SLM capable microscope would be 💯, since I could retrofit it to become an in-house microfluidic chip factory.
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RT @BioinfoAdv: 🧠Recently published in Bioinformatics Advances: "A Kolmogorov metric embedding for live cell microscopy signaling patterns"….
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RT @Ankita_13Jha: I am beyond excited to present my postdoc work to the community. I have synopsis of this paper on the bluesky account (or….
biorxiv.org
Cells under high confinement form highly polarized hydrostatic pressure-driven, stable leader blebs that enable efficient migration in low adhesion, environments. Here we investigated the basis of...
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