https://t.co/b9yzMQ4dxq RB-TnSeq meets the archaeal domain!! I am very proud of this final chapter of my thesis work with @redoxgeek. A few notes on my fav results, & a lil on how this project came to be

I know—it snuck up on us too… The application deadline for the 2025 Microbial Diversity Course @MBLScience has been extended to 10 February. If you would like to participate in a life-changing experience, please apply: https://t.co/TlyVZYYTD7

Here is the sketchbook page you can see me working on!! Mosasaur, plesiosaur, ghost shark, basking shark, and the sandworm from Beetlejuice :)

https://t.co/8XK2ZfhrEy Shoutout to @anaerobicemily. Everyone take a look for inspiration for being a great person while also merging being a talented scientist & artist.

🔔Apply now!🔔 January 27 is the final day to submit applications to the MBL's 2025 Physiology Course. This seven-week lab course has educated generations of leading biologists and fostered groundbreaking discoveries. Learn more: https://t.co/9JXXl2PAV4 #ScienceStartsHere

They named a new elementary school building in Missouri after the janitor at their school. This rocks so much

Excited to announce that our 2025 Advanced Research Training Course schedule is up and applications are open! 🎉🎉🎉 See you in Woods Hole! https://t.co/9cAcnqoAfG

#Methane emissions are more visible from outer space. "A comparatively small number of highly emitting persistent methane sources are responsible for a large share of global methane emissions". What are they? #climate #ClimateScience #CH4 @EGU_ACP https://t.co/XFTFeS46uT

Lab automation will make science faster! The lab automation:

Post-doctoral associate position open in microbiology at university of Minnesota with Job ID 365152

This is 100% my experience. It also makes sciencing so much more fun.

All it took was moving to the east coast then two weeks later waking up at 4 am to drive 2.5 hours then take a train 1.5 hrs then the subway and a tram and waiting in line for 2.5 hours then trekking back home (by myself) and also this was my first time in NYC 😂

Fulfilled a 10 year dream of attending a CJ Hendry exhibit today 🥹🥰

I was able to develop the libraries and ship them out to Berkeley where I spent 6 months in the @DeutschbauerLab optimizing and performing fitness assays and analyzing data with help from Adam Deutschbauer @HansCarlson3 & Morgan Price!

Thus coming into the 3rd year of my PhD I reached out to Adam @DeutschbauerLab and proposed applying for the SCGSR and developing the RB-TnSeq library in Methanococcus and working with him to run some fitness assays. I was awarded the SCGSR & some fitness assays became many….

I started graduate school then and I missed having this resource in a related species since working with Methanococcus, especially since COVID hit right after finishing rotations and data analysis and reading were the options available

A note on my motivation for this project - When I was an undergrad I would often browse Desulfovibrio genome info and look at RB-Tnseq data for homologs of genes of interest in other bacterial species on the FitnessBrowser from the @arkin_lab @DeutschbauerLab. So helpful!

I hope that this large dataset can be a useful tool for the archaeal community & inspire others to generate other RB-TnSeq libraries in their favorite microbe (even if it’s a strict anaerobe, that likes high concentrations of H2 and sulfide it’s possible!) 🥳

This is just a sampling please check out the paper for other cool stuff and follow up experiments! Much more work to be done. Please reach out if you have questions or want help looking at fitness data for your favorite gene 👩🏼‍💻

When looking at sulfite stress we found that 0.2 mM sulfite can actually be used as the sole S source if you over-express the gene encoding dissimilarity sulfite reductase-like protein (Dsr-LP)! Despite lacking the F420 oxidase domain found in Fsr, it’s functional!🤯

To my surprise, I found temporary oxygen exposure isn’t so bad when sulfide isn’t around to be oxidized to sulfite. Although there are a few genes affected