Jake Watson
@jakefwatson
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Neurophysiologist @ISTAustria, previously @MRC_LMB. Opinions expressed are obviously my own.
Vienna, AT
Joined March 2017
Just to note - some of these ideas are not new, but seem to have been lost in the sands of time. Happy to hear any feedback and discussion on the topic!.
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But not all is lost. We propose a way to detect the detection limit, allowing informed analysis of your data. With this we see that at CA1 synapses, most minis are below usual noise levels (<6 pA). So be careful in concluding specific changes, no matter how obvious they may seem.
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We can confirm this on real-world recordings. Specifically changing mEPSC amplitudes by altering the driving force across the membrane shows up as a change in mEPSC frequency!
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Most alarmingly - if we change our simulations to specifically increase the amplitude of input events, the main effect is an increase in detected frequency! So be very careful about concluding "specific" changes in mPSC amplitude or frequency.
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Using simulated data, we see that even subtle changes in noise levels massively affect the 'measured' values. So if recordings don't all have the same noise levels, it would be easy to observe false changes.
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mPSCs are small and embedded in noise, so a lot of events are undetected. The problem is - when studying synaptic changes, undetected events also change, seriously complicating interpretation.
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Recording synaptic minis (mEPSCs)? We have a new paper trying to better understand how recorded datasets match synaptic changes. In short, they don't. More info and the preprint below:
'Mini analysis' is an unreliable reporter of synaptic changes #biorxiv_neursci.
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RT @JGarciaNafria: Delighted to get this out!we describe bitopic binding mode to the dopamine 3 receptor, including a reorganization of TM1….
nature.com
Nature Communications - Developing subtype selective drugs for GPCRs is a major focus of research. Here, Arroyo-Urea et al. point to an unexploited selectivity site in aminergic receptors, as seen...
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RT @igreger1: Here is a short summary of our new paper, describing how protons shape AMPA receptor conformation, kinetics and synaptic loca….
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RT @RTMojtaba: Beyond CONNECTOMICS, we have used our developed #LICONN technology to e.g. study functional connectivity rules in human and….
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RT @igreger1: Our latest review on glutamate receptor organisation at synapses, with Imogen and Jake @jakefwatson . .
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Beautiful analysis of human circuit architecture from @YangfanPeng et al. - it does not get more careful than this. A great demonstration that understanding circuit function requires consideration of individual neurons!.
🧠 Curious about how neurons are wired in the human cortex?. Our @ScienceMagazine study tested over 7,000 connections and revealed key differences from rodents:."Directed and acyclic synaptic connectivity in the human layer 2-3 cortical microcircuit". 🧵👇
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🔽 Structural Biology Postdoc position with a super team, in sunny Spain on exciting biology and technology!.
Keen to work on orphan GPCRs through cryo-EM? We are looking for a postdoctoral researcher.
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Great to have Franz @FMittermaier and Jörg Geiger here at @ISTAustria . Keep an eye out for the incredible portfolio they are putting together on microcircuits of the human brain!.
Grateful to @jakefwatson from the Jonas lab for inviting Prof. Geiger and me to speak at IST in Austria yesterday! I was excited to present our data on HUMAN axonal signaling 🧠⚡️. Huge thanks for the warm hospitality! Check out our preprint (link in comments) for more insights.
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RT @t_malinauskas: We show that GluD1 binds to GABA, an extraordinary feature for iGluRs. GluD1 activation produces long-lasting enhancemen….
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RT @isabelbeets: Excited to share 2 postdoc positions in my lab! Are you interested in studying the neuropeptidergic connectome and behavio….
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RT @igreger1: Our latest work an AMPA receptor/TARP therapeutics now published - congrats to all involved, prospective students interested….
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RT @CPW_Plymouth: Apply now!.38th Microelectrode Techniques for Cell Physiology Workshop, 30 August – 13 September 2023.Marine Biological A….
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RT @JGarciaNafria: @BeatrizHergued1 hablando de mujeres y ciencia. 😍😍😍😍. Opiniones certeras para conseguir esa paridad! .
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