How to attract the attention of your public after a coffee break. Andreas Tolias, you nailed it! #neocortex #brain #rngs19 #patchseq

Functional connectome with transcriptomics from Andreas Tolias ! #RNGS19 #patchseq

9/12 Two more things: Lots of data on other cell types. One challenge for electrophysiologists is challenge is the identification of non-beta-cel types. We show #patchseq can help refine models to better identify glucagon-secreting alpha-cells based on their e-phys.

7/12 In type 2 diabetes (T2D), beta-cells are impaired. Studies have reported altered single-cell gene expression, but it’s hard to know which are important. #patchseq can help with this by simultaneously studying changes in expression and function…

Our #singlecell #pancreas #patchseq preprint is out: "Pancreas patch-seq links physiologic dysfunction in diabetes to single-cell transcriptomic phenotypes" @bcellorg #QuakeLab #KimLab @czbiohub

I’m seeking a research assistant to work on an @mssocietyuk funded project at the @UofGlasgow @iiiglasgow along with @BiottofnI and others. Experience in #electrophysiology and/or primary #cellculture essential. #liveimaging #bioinformatics #Patchseq.

Dr Dayne Beccano-Kelly (UK DRI at Cardiff) is looking for a research associate to join his new lab at UK DRI at Cardiff 🏴󠁧󠁢󠁷󠁬󠁳󠁿. Assessing synaptic activity in Parkinson’s using cutting edge tech - PatchSeq, Optogenetics iPSC & knock-in models 🧠. 🗓️ 20 Mar 👉

Excited to talk at the 32rd Head Group Meeting: Craniofacial & Neural Development @UCLchildhealth in Jan 2020! . I’ll be presenting new work on neuronal polarisation in vivo, also looking forward to hearing the latest on olfactory bulb #patchseq from Marcela Lipovsek @GrubbLab

We are soon advertising a position (#postdoc/staff #scientist) in #Data Management, #Computational analysis and/or #Bioinformatics. Help us build a program that combines datasets from cell->tissue->patient!. #biobanking #genomics #physiology #patchseq.

We are delighted to publish this #Neuroscience #Protocol for #PatchSeq of Small #Interneurons , by Dr. Matthew S. Grubb and colleagues @GrubbLab @dev_neuro .

(1/11) Andrés Crane’s thesis work in the lab with Suresh Jetti using PatchSeq to define the targets and rules for single neuron RNA editing in Drosophila motoneuron subtypes is now available as a preprint:.

Hey, do you want to get electrophysiological recordings and transcriptomic data from the same small cells? Tips and tricks and (really) ALL the details in our newly published #Patchseq protocol! @LorcanBrowne @dev_neuro @KingsIoPPN . ⬇️⬇️⬇️.

#Patchseq - the next frontier:

5/12 To characterize molecular differences that underlie synapse diversity, I established Isoform-Patchseq in collaboration with core facilities at @MIT. Special thanks to @Scientifica_Int manipulator which made my life easy to collect these challenging patch-sep samples!

1/12 Here’s our paper on the dual e-phys/scRNAseq of >1300 human islet cells. I can’t emphasize enough the massive effort by @XiaoqingDai who patch-clamped ALL the cells and @JoanCamunas who did ALL the sequencing/bioinformatics. #patchseq .

Cell Locator: @3DSlicerApp-based desktop app that manually aligns specimens to annotated 3D spaces developed for the @AllenInstitute via @kitware . #openscience #neuroscience #cellscience #patchseq.

Our patchseq data has been used in a few papers. Nice to see it used here to show differences in Na(v) current in beta cells depending on HNF1A expression. We’re actively working to make this and other data more broadly usable and available. Stay tuned.

Our PatchSeq study is published! It uses transcriptomics, ephys, and morphologies from individual cells (>4000!) to classify 🐭 cortical GABAergic neurons. Great work by @gouwens,.@staci_sorensen, and many colleagues @AllenInstitute! . #OpenScience

@suresh__jetti and colleagues paper from the lab on PatchSeq approaches to characterize differences in Drosophila tonic-like Ib and phasic-like Is motoneurons is now published in Neuron – check it out from the link below.

Our new paper in @Nature_NPJ highlights transcriptomics of #Parkinson’s neurons in a dish (42 donours). Diff expression always shows something. but if it overlaps between methods (#iPSC, #iN, #PatchSeq, #10X, #bulk), gotta be real, right? 1/9