RT @cebcambridge: Science today has little understanding of how life functions at low temperatures. That is about to change. CEB is working….

RT @cebcambridge: Our Head of Department, Professor Clemens Kaminski, gave a fascinating lecture to our alumni on the history of the light….

RT @cebcambridge: Ever wondered how Antarctic fish survive in freezing waters? 🐟❄️. With new funding @UKRI_News, we are creating the micros….

Superresolution microscopy at sub zero temperature: 2 year post doc position available in @group_laser in @cebcambridge and @BAS_News doc to build instruments to study life in the Antarctic. 🔬🥶🐟🧑‍🔬

In a great collaboration with @rogerrubio from the @DiMicheleLAB1 we have used the method for the 3D visualisation in multiple colours of the re-organisation of membrane-bound DNA nanostructures on unilamellar giant vesicles.

The method handles uneven phase shifts between illumination patterns, as well as sample movement and noise and is less prone to the artefacts than analytical reconstruction methods. It offers similar sectioning capability to confocal microscopy at higher speeds.

Our reconstruction algorithms are open-source and use deep residual channel attention networks and video #superresolution to process sectioned SIM images with higher performance and speed than other methods.

The method combines interferometric pattern generation with machine-learning to achieve high-contrast, high-speed reconstruction of image data in multiple colours and in real-time.

A Michelson interferometer is a legendary instrument among physicists. In their new paper, published in #OSA_BOEx, @edward_n_ward and @r_m_mcclelland, from @group_laser in @cebcambridge use it to create an optical sectioning microscope.

Wonderful news and richly deserved! Delighted for you.

Wonderful- and very long term! - effort led by Francesca van Tartwijk with great contributions from Lucia, Ioanna, Julie and many others from the Kaminski, Holt, Franze, and St. George Hyslop labs. 👏🥂🪇🎉🥳🍾.

Overall, this work supports the idea that FUS’ role in development can be compromised by familial ALS-associated mutations, and therefore that familial ALS may have a developmental aspect.

We also see that this loss of F-actin in the growth cone leads to a considerable decrease in their stiffness when mutant FUS is present, whilst axonal RNA transport is unaffected.

The apparent defect in cytoskeletal organisation observed in vivo, is associated with a loss of F-actin in the growth cone when mutant FUS is present.

Using an in vivo Xenopus model, we first show that expression of FUS(P525L) or of the hypomethylation mimic FUS(16R) reduces axonal complexity. The former is also associated with a ‘looping’ phenotype, which may impair the transition from the elongation to the branching stage.

Our preprint on the ALS/FTD-associated RNA-binding protein FUS’s role in axonal development has now been published in Journal of Neuroscience! We show that expression of FUS variants compromises the integrity / complexity of the distal axonal cytoskeleton.

Bittersweet farewell for our great @LuMeng2020, the godfather of organelle imaging and post doc for 7 years in @group_laser and @mng_ceb. On to great new things, Prof. Lu, and off to a cracking career in @PKU1898! Well done, Meng, and richly deserved! @cebcambridge

An epic effort by @AScheeder of @group_laser with superb guidance from our @IwannaMel and great help from @mng_ceb. On to more exciting collaborative stuff between @cebcambridge and @UnivCamPharm. 🎉🥳🍾.

On Gram-positive cells on the other hand we saw internalisation of cFLs directly into the #bacteria, although the process is sporadic and is not successful for all cFL - bacterial membrane encounters.

With high resolution #SIM imaging we could see how cFLs disrupt the outer membrane on individual gram negative #bacteria, causing membrane detachment and at the same time improved delivery of #antibiotic agents.