Masatoshi Inoue
@feynman1104
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Neuroscience/Bioengineering, Assistant Professor @WUSTL Recruiting postdocs, grad students, & research tech.
Joined September 2012
[Plz RT] Our lab is looking for Research Assistant and Postdoc Researcher! We focus on developing and applying innovative optical molecular technologies to investigate neural circuits underlying maladaptive social behaviors.
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New paper is out today in @CellCellPress! New faster, sensitive and multicolor genetically encoded calcium indicator: Thanks to a great team, especially Atsuya Takeuchi, Satoshi Manita @KarlDeisseroth, @kazkitamura and Haruhiko Bito!
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I'm pleased to announce that I'll be joining the Department of Radiology @MIRimaging @OpticalRadLab at WashU @WUSTL @WUSTLmed @WashUONR as an Assistant Professor in early 2024! I couldn't be more excited to join the amazing WashU neuroscience and imaging community. (1/4)
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先日神経化学会誌にカルシウムセンサーについて思うことを書かせて頂きましたので、ご笑納ください。コメント大歓迎です。.
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ギリギリの告知ではありますが、明日東大駒場で16時から加藤英明さん(@emeKato )オーガナイズでセミナーをします。.カルシウムセンサー、神経科学、イメージング、タンパク質デザインに興味がある方は参加して頂けると幸いです。
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Excited to publish a review article about the history and limitations of current available GECIs, and the future prospects for engineering further improved GECIs.
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New paper @CellCellPress! (1) Solving ChRmine structure, (2) Structure-guided design of three ChRmine variants, rsChRmine (red-shifted & reduced blue shoulder), hsChRmine (high-speed), and frChRmine (faster & red-shifted), (3) for all-optical neuroscience.
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実験医学11月号に先日のCell論文の日本語総説を書かせて頂いたので、見て頂けると幸いです。遺伝子にコードされたカルシウムセンサーのことについて知りたい人には特におススメです。
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Viral Vectors for Neural Circuit Mapping and Recent Advances in Trans-synaptic Anterograde Tracers: Neuron
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We are looking forward to conducting synergistic research through our collaboration with S Inoue Lab in the Department of Psychiatry @s_k_inoue as a twin Lab and are excited for the journey ahead. (4/4)
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New paper out @CellRepMethods with @SakamotoMasayu,@kazkitamura. 1. Develop novel green GECI, G-CaMP9a. 2. Generate first Flp-dependent Green GECI mouse line for two-photon in vivo imaging. Thanks to a great team, especially Haruhiko Bito!.
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@tsuyomiyakawa 余計なお世話かもですが、東京が心配で単純に指数関数フィットした今週の東京の動向は今ロックダウンしているベイエリアより増加してしまうんですよね。神奈川はまだ指数関数に入る前？
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ブラックラボで温かいおもてなしを受けました😃. Thank you for showing me amazing Hideaki's lab👩🔬👨🔬. Hope your lab makes big finding soon. @emeKato
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光でGPCR活性を可逆的に制御する。. Branched Photoswitchable Tethered Ligands Enable Ultra-efficient Optical Control and Detection of G…
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Enhancer-AAVs allow genetic access to oligodendrocytes and diverse populations of astrocytes across species
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My paper was selected for the Cover of Neuroscience Research Cover Art of "Optical probing complex brain circuit dynamics with GECIs".
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Relationship between simultaneously recorded spiking activity and fluorescence signal in GCaMP6 transgenic mice
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Looking forward to my poster session (PSTR513.23) on Wednesday morning #SFN2023 @SfNtweets. Also, feel free to DM me to meet or chat at the poster session for the Inoue Lab coming soon 2024 @WUSTL.
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この度は再々インタビューありがとうございます。試聴者としてJob interviewの移動中にNeuroradioを聞くのがすごく楽しみでした。ぜひM Inoueラボについて興味がある方は気軽にお問い合わせください。.
#65 Dual-career PIs in St. Louis.Washington University in St. Louisでそれぞれ独立PIになる井上昌俊さん（@feynman1104）と井上清香さん（@s_k_inoue）がゲスト。.最新ジョブハント事情、パートナーとの就活、WashUでの研究プラン.#NeuroRadio #神経科学 #ポッドキャスト.
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Oh!."Self-inactivating" rabies viruses are just first-generation, ΔG rabies viruses ／(^o^)＼ﾅﾝﾃｺｯﾀｲ.
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OptoGRC was ver exciting and inspring conference. But, I tested posiive for Covid this evening. Tested negative until last evening. Anybody who I hung out with at @OptoGRC should get tested.
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因みにKarlの本もaudible(で聴くのがのがお勧めです。内容も彼の20年に渡る研究と臨床のBiography とい���感じで良いのですが、普段の100倍位の美声で収録されていました。.
Andy HubermanのポッドキャストにKarl Deisseroth (ep14,15のゲスト、井上Masaさんのボス)がゲスト出演。精神医学的な興味やChR2等のツール開発、キャリア等、２時間じっくり対談してるので要チェック！.(タイミング的に本の宣伝の意図もあるのかな、と) 萩.
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Great work detecting subthreshold voltage imaging in vivo. Population imaging of neural activity in awake behaving mice
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Large-scale voltage imaging in the brain using targeted illumination
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Honored and grateful to be a part of such a wonderful team!.
Congratulations to this constantly amazing group of students, postdocs, staff & collaborators including the indomitable @yoon_Seok_Kim, with @emekato, @sokosoko_seeya & our whole structure team! A new mechanism for K+ selectivity->new tools for biology.
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Light-guided sectioning for precise in situ localization and tissue interface analysis for brain-implanted…
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研究室の友人のLinlin @LinlinZFanがMITで2024年1月から独立します。 Ambitious なプロジェクトを用意していると聞いています。彼女は人柄もナイスなので、興味ある方は是非コンタクトしてみてください！.
Postdoctoral fellow in the Fan Lab at MIT.
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Video overview of past three papers related to calcium indicators. Thanks for tax payer!.
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Deisseroth labから最近UCSFで独立したRitchie Chen @ritchie_chenが神経科学の人を募集しています。systems neuroscience, computational neuroscience, やneuralengineeringというキーワードにピンと来た人は連絡してみると良いと思います。.
Postdoctoral fellow in systems neuroscience at the University of California, San Francisco.
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DeepCINAC: a deep-learning-based Python toolbox for inferring calcium imaging neuronal activity based on movie visualization
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取り上げて頂きありがとうございます！ざっくばらんにサイエンスの話をしているので、聴いてみてください。.
#14 Dual-career at Stanford: Dr. Sayaka Inoue and Dr. Masatoshi Inoue – Part 1.というわけでShah・Deisserothラボから井上夫妻(@s_k_inoue , @feynman1104)をお呼びして、論文の裏話、スタンフォードで神経科学を研究する醍醐味などについて伺いました。後半に続きます。.
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Huge Congrats @KarlDeisseroth.
Join us NOW to see the winners of the Basic Award!—Dieter Oesterhelt of @MPI_Biochem, Peter Hegemann of @HumboldtUni, and @KarlDeisseroth of @StanfordMed—for “light-sensitive microbial proteins and #optogenetics”. #Lasker2021 #LaskerLaureate #LaskerAward
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Got dungeness crab from half moon bay fisherman. Cooked and it was sooo delicious 😋
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LoogerからSoluble proteinのPBPを改変したAchセンサー。. A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
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ついに出ましたね。paQuasAr3の周りにTSモチーフが4つあるのがやや気になる. Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics
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Time to decode neuronal firing pattern.
From the Schnitzer lab: A multi-beam two-photon microscope enables imaging of calcium activity or neurovascular dynamics in the mouse brain with millisecond-scale temporal resolution.
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Good review article for wireless/battery-free neuro-technologies.
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Spatiotemporal limits of optogenetic manipulations in cortical circuits
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Hijacking of internal calcium dynamics by intracellularly residing rhodopsins
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Taishoken in San Mateo. Pretty authentic Japanese noodle 🍜 😊
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Low Computational-cost Cell Detection Method for Calcium Imaging Data
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Marston, D.J., Vilela, M., Huh, J. et al. Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis. Nat Chem Biol (2020).
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All optical experiment CoChR+jRGECO pair in Zebrafih. High-throughput cellular-resolution synaptic connectivity mapping in vivo with concurrent two-photon optogenetics and volumetric Ca2+ imaging
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Especially, rsChRmine enables in vivo 3-color "all-optical type-to-type" with our XCaMP sensors (blue & green): causally interrogating how distinct cell-types communicate with each other. This is kind of dream experiment when I was grad student.
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A database and deep learning toolbox for noise-optimized, generalized spike inference from calcium imaging.
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楽しみにしております！.
8月8日（火）井上ご夫妻による医学研セミナーを開催.15-16 井上昌俊博士@feynman1104.全光型マルチモーダル機能マッピングで解き明かす社会行動の神経メカニズム.16-17 井上清香博士@s_k_inoue.性周期に伴い雌の行動を調節する神経メカニズム.医学券での対面式のみです！参加ご希望の方ご連絡ください.
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A fluorescent sensor for the real-time measurement of extracellular oxytocin dynamics in the brain
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Congratulations to @liefefenno, Charu, and @Yoon_Seok_Kim on their latest paper in @NeuroCellPress! I'm so grateful to have been a part of this project. Check new red GECI "sRGECO" which is engineered to reduce aggregation.
Stoked! that our latest work is out @NeuroCellPress: a large viral toolbox for intersectional expression of molecular tools in cells defined by multiple parameters. 1/5
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Rapid single-wavelength lightsheet localization microscopy for clarified tissue
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Distinct roles of parvalbumin and somatostatin interneurons in the synchronization of spike-times in the neocortex
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現代化学の最新号で留学中の『Karl Deisseroth研』の紹介記事を執筆しましたので、ご興味のある方は是非！.
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Subcellular resolution 3D light field imaging with genetically encoded voltage indicators
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Compared red-shifted opsin performance under 2p. In Vivo Submillisecond Two-Photon Optogenetics with Temporally Focused Patterned Light
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I'm always surprised that imaging light intensity is much higher than opsin stim. light intensity despite less tissue damage.
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Selective Control of Synaptically-Connected Circuit Elements by All-Optical Synapses
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I agree with this point. That's why we developed XCaMPs, which showed faster, larger SNR, linear to spikes than GCaMP6. But, maybe faster GECIs is still desire.
An important analysis of the spike to Ca transient (actually to GECI fluorescence) transform and methods to deal with its nonlinear nature. From @ShaulDr & co.
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I am beyond thankful for the support from my incredible mentor @KarlDeisseroth, and Haruhiko Bito, colleagues & family - I wouldn’t be here without my amazing community. (3/4).
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Dopamine waves as a mechanism for spatiotemporal credit assignment
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Dual polarity voltage imaging reveals subthreshold dynamics and concurrent spiking patterns of multiple neuron-types
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Congrats on Masa! Fair comparison of available GEVIs because authors are end users (not developer).
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The M Inoue lab will develop optical molecular tools to explore the contribution of both local and global circuits in the brain to social information encoding and animal behavior. We’re hiring at all levels from tech to postdoc. Please RT and/or reach out if interested! (2/4).
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A wireless closed-loop system for optogenetic peripheral neuromodulation
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Congratulations Isaac and team!.This work shows the true worth of d lab’s research.
Will some principles in neuroscience require a brainwide, fast, cellular-level perspective for discovery? We’re delighted to share our new paper reporting an outcome of this approach: @Nature co-led by @SamVesuna and me, and mentor @KarlDeisseroth. (1/n).
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Neural Cell Types Tied to Autism Identified in Single-Cell Study
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Multimodal activation of GPR68 (OGR1) probed with a genetically-encoded fluorescent reporter
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@AndrewHires @gottapatchemall 2-3 uW 385 nm is enough to detect FP signal because XCaMP-B is bright. Indeed, I haven't seen any significant tissue damage so that I was able to record XCaMP-B signal for six months.
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