Liu et al. present a dynamic graph structure that adapts to varying data quality, enabling more robust DNA sequence assembly and reconstruction from highly noisy or low-coverage data, with broad...

Gut virome analysis is limited by tools that struggle with viral diversity and novelty. Yang et al. present SinProVirP, a signature-protein-based profiler that accurately quantifies known and novel...

Russell et al. construct a high-throughput experimental and computational pipeline that calculates nuclear ploidy from microscopy images and outputs a representative ploidy map showing the spatial...

Djamshidi et al. describe a strategy that they call fast, accurate and more efficient (FAME)-CRISPR utilizing pan-histone deacetylase inhibitors and engineered virus-like particles to improve the...

5′ UTRs regulate the translation of the coding sequences and are often mutated in patients with autism and other diagnoses. However, their functional impacts are unclear, especially in the brain....

Burnell et al. develop a computer-based method to count the number of cells in a well of organoids from a regular microscope image. They compare its use to standard counting techniques and test it in...

Li et al. present a framework integrating automated behavioral tracking and computational modeling to dissect mouse depression tests. They reveal that the forced swim and tail suspension tests engage...

Tan et al. introduce a one-step method to produce barcoded rabies viruses that shortens timelines, boosts rescue efficiency, and eliminates background contamination. The approach yields barcode...

Tan et al. combine barcoded rabies tracing with single-cell RNA-seq to map local and long-range inputs to single mPFC neurons, revealing cells with high local innervation and a positive link between...

Sabnis et al. use non-invasive video recordings of mice to develop a method using supervised machine learning to automatically quantify seizure severity. This approach provides a scalable and...

Wang et al. introduce DVR-NeMF, a neural magnetic field framework that reconstructs dynamic 4D MRI in real-time level with high fidelity. By leveraging spatiotemporal priors, the method enables...

Lou et al. present stLVG, a lightweight vector-guided graph model for spatial multi-slice and multi-omics data integration. stLVG incorporates distance- and angle-based weights to capture spatial...

Rice et al. report Fuzzion2, a high-performance program for targeted gene fusion detection in unmapped read pairs. Sensitivity meets or exceeds that of de novo callers, making Fuzzion2 well suited...

Cramer et al. present TRACE-QC, a method for quality control of time-course imaging in 3D cell cultures. Using iterative Procrustes analysis, it reorders and aligns spheroid positions, corrects...

Krieg et al. develop melanoma brain metastasis models based on cortical brain organoids. They demonstrate the potential for therapeutic applications, screening a library of anti-cancer compounds and...

Williamson et al. present FlickerPrint, an open-source computational analysis tool for measuring the interfacial tension and bending rigidity of soft bodies from their shape fluctuations in time-se...

Shevade et al. present CAT-ATAC (CRISPR and transcriptomics-assay for transposase-accessible chromatin), a single-cell sequencing assay that profiles gene expression, chromatin accessibility, and...

Zhang et al. develop two types of multiplexed miniaturized two-photon microscopes (M-MINI2Ps) that increase imaging speed via beam multiplexing while preserving spatial resolution and demonstrate...

Forbes et al. benchmark human read removal methods for viral and microbial metagenomics. Using synthetic and microbiome datasets, along with a set of benchmark methods, they demonstrate that a custom...

Teles-Reis et al. present EyaHOST, a modular Drosophila system that enables clone generation in the eye disc with independent genetic manipulation of other tissues. EyaHOST uses an Eya-KD “kick-out”...