Diego Presman
@PresmanLab
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Molecular/Cellular Biologist. Group leader at IFIBYNE-CONICET - UBA. Trying to understand how transcription factors work. Hard to do science nowadays...
Buenos Aires, Argentina
Joined March 2021
Happy to share the latest publication led by @PabloFuentesP, G. Hager, and @EstebanezLab, with talented co-first authors @Andrea_AlegreM, @AlbaJimP1, and @aguslafuent. This one feels special to me, as I explain in this thread below.... 🧵🧵🧵 https://t.co/QAdQ2baOss
academic.oup.com
Abstract. The glucocorticoid receptor (GR) is a leading drug target due to its antiinflammatory and immunosuppressive roles. The functional oligomeric conf
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Watson – who won the Nobel Prize for his role in divining the structure of DNA; was instrumental in initiating and propelling the Human Genome Project; and who became notorious for his history of racist and sexist comments – has died at 97 Read the full story:
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Aplaudimos el aumento conseguido por el Garrahan y vamos por un aumento parecido para ciencia! Los becarios doctorales de Agencia necesitan un aumento del 108% y el resto del sistema científico necesita un aumento del 62%.
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Too many people to thank, but all this journey would not have been possible without the mentorship of Adali Pecci, @VLeviLab, the Gordon Hager lab, and crucial collaborations with the Estébanez-Perpiñá team, who joined us in this fight. Special thanks to my PhD student, Agustina!
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In summary - GR is mostly dimeric upon ligand activation - GR’s final conformation might be a tetramer - GR-GR contacts depend on a complex, intertwined crosstalk within all structural domains: LBD, DBD, and likely the NTD - Is transrepression even necessary to explain GR action?
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Finally, Eva and Pablo’s team was able to crystallize GR’s LBD in a tetrameric form, giving us a unique opportunity to probe and test different oligomerization surfaces on GR. They even managed to capture GR tetramers in vitro with a GR DBD-LBD fragment! https://t.co/QAdQ2baOss
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By combining X-ray crystallography and live cell studies, @AlbaJimP1 and @Andrea_AlegreM were able to find new surfaces of GR-GR contacts, suggesting that several types of dimers can coexist in the cell https://t.co/TgMPGpPpdn
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What was really missing, even though we already had live-cell experimental data, was the crystal structure of the tetramer. Here comes Eva (@EstebanezLab) and Pablo (@PabloFuentesP) to the rescue!...
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The idea that GR is a tetramer was received with high skepticism, to put it mildly, even though we demonstrated increased transcriptional activity for a constitutive tetrameric mutant receptor (GRtetra), https://t.co/SKOnxqlhnd
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Back in 2016, we were able to quantify the oligomeric state of GR on a tandem array of DNA elements within live cells, and we were surprised to find that GR was actually a tetramer when bound to chromatin. https://t.co/wHpw43TZFY
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Fast forward to 2021, we also found that a mostly monomeric GR mutant (GRmon) was unable to activate genes and bind to chromatin, suggesting that most of GR activity was done by GR dimers https://t.co/SlWJXqtWJS
nature.com
Nature Communications - Glucocorticoid receptors (GR) are thought to bind DNA as dimers or monomers, to regulate different transcription pathways. Here, the authors perform genome-wide studies on...
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By 2014, we confirmed that the GRdim mutant, allegedly monomeric and thus the main evidential basis for the claim of monomeric GR action, was actually a dimer. Where are all the monomers? https://t.co/3UokfBjPpA
journals.plos.org
The glucocorticoid receptor's oligomerization state is revealed to not correlate with its activity; this challenges the current prevailing view that this state defines its transcriptional activity.
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In 2010, togehter with @VLeviLab we applied the N&B assay to quantify, for the first time, the oligomeric state of GR in live cells. Surprisingly, we find that the population of GR is mostly dimeric in the nucleus. Where are all the monomers? https://t.co/33Ct7FqyL7
journals.plos.org
Background The glucocorticoid receptor (GR) is a transcription factor that regulates gene expression in a ligand-dependent fashion. This modular protein is one of the major pharmacological targets...
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This paper marks the culmination of a 15-year (and 7 papers) journey to challenge #Glucocorticoid_Receptor (GR) dogma. The classic paradigm of GR action states that monomeric GR and dimeric GR regulate a different set of genes
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Este 26 de octubre defendamos la Ciencia, la Tecnología, la Salud y la Educación en las urnas. No votes contra la Ciencia Argentina 🇦🇷
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🎯 With absolut joy we share our newest #NucleicAcidsResearch on Glucocorticoid Receptor Multimerization. Enormous team work @PabloFuentesP Gordon Hager @AlbaJimP1 @Andrea_AlegreM @aguslafuent #TomJohnson @PeraltaMNuria @PresmanLab #JFRecio #JaimeRubio @BQBiomedMolUB 🥳🚀
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We are seeking assistance in locating a missing researcher in Germany. If anyone is based in Germany or has contacts in the area who might be able to help, please get in touch as soon as possible. Any information or support you can provide would be greatly appreciated.
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Necesitamos la Ley de Emergencia en Ciencia y Tecnología! Se trata este miércoles 8 de octubre. Pedile a los diputados/as que no sean cómplices del cientificidio. @JuanmaLopezAR @FCarbajal2021 @albertoarruaok @drfernandezok @ruizyamilal @colovancsik @AgustinxDomingo @gcipolini
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El Congreso trata hoy la Ley de Emergencia del Sistema Científico, que puede poner fin al desmantelamiento de la ciencia que impulsa el gobierno. Argentina necesita ciencia para crecer, no hay otro camino posible. Hoy se juega nuestro derecho a un futuro mejor.
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🚨¡Ayúdanos a salvar a la ciencia argentina de la destrucción de Milei! Sumate al TUITAZO para exigir a los diputados la aprobación urgente de la #LeyDeEmergenciaCientífica
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