"Cancer cachexia" affects 30% of cancer patients - 80% for advanced cancers - and yet few have heard of the term. Assoc Prof Louise Cheng (@LabFly), @HyunKoMD and cancer survivor Sudhir, have explained this muscle wasting condition to @3RRRFM. Listen: https://t.co/LDeZH61Xi5

Congratulations @KatMitchell_Sci on this beautiful story on the Hippo and TNF/JNK pathways and epithelial tumour suppression. Great work by you and the whole team @PeterMacCC @MonashBDI

Thanks so much to all the people who helped with this research, it was a team effort and something I’m very proud of😀! A big thanks to @HarveyHippoLab @JonathanPojer @ECBrooks92 Joep Vissers AJ & Jan Schröder 🧵10/10

Thus, AP-1/CtBP eliminates defective cells and prevents tumour initiation by acting in parallel to Yorkie/Scalloped to repress expression of a shared transcriptome 🧵9/10

If gene repression by AP-1 and CtBP fails, neoplastic tumour growth ensues, driven by Yorkie and Scalloped 🧵8/10

The C-terminal binding protein (CtBP) is required for AP-1 mediated repression and we show that Fos and CtBP form a physical complex 🧵7/10

In neoplastic cells, Yki accumulated in the nucleus, suggesting defective Hippo signalling. However, Hippo pathway target genes are not elevated as they are repressed by AP-1 🧵6/10

We show that both Jun and Fos limit the growth of defective neoplastic cells. Loss of fos in neoplastic cells results in tissue overgrowth and elevated Hippo pathway target gene expression 🧵5/10

Using CRISPR/Cas9 genome editing we generated new mutant alleles of both jun and fos in Drosophila melanogaster and carried out some sophisticated genetic experiments 🧵4/10

We utilised targeted DamID to reveal that the AP-1 transcription factor Jun, and the Hippo pathway transcription regulators Yorkie and Scalloped bind to a set of common target genes involved in organ growth 🧵3/10

Previously, it had been found that JNK/TNF signalling limits the growth of defective neoplastic cells, but it wasn’t completely understood how this happens 🧵2/10

Very excited to share our #preprint in @biorxivpreprint in which we found that the transcription factors of JNK and Hippo pathways control epithelial integrity by regulating an overlapping transcriptome 🧵1/10

Pleased to share our latest paper describing @Bioconductor package for analysis of DamID sequencing data. Congratulations Caitlin Page who finished this BEFORE starting a PhD with me this year! Damsel: Analysis and visualisation of DamID sequencing in R

#Hippo2023 has been 5 yrs in the making - 3 postponements, a pandemic and an online conference later it’s been great to reconnect with many friends and hear the latest breakthroughs. Here is ⁦@HariharanLab⁩ with his F1 ⁦@taponlab⁩ and me, and the F2 generation!

Beautiful talk by @KatMitchell_Sci on the co-regulation of transcription by TNF and Hippo pathways in neoplasia at the Hippo Signalling Conference 2023 👏

Incredibly pleased to announce that Dr @KatMitchell_Sci has taken out Peter Mac MicroART 2023 with her stunning image "In the Eye of the Beholder". Dr Mitchell uses the fruit fly to investigate how the Hippo pathway controls growth and #cancer. More: https://t.co/lFFWsXQQzf

Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcription

Some great snaps by Kat @KatMitchell_Sci and AJ @linkinbios of fly eyes that lost their way during development! Both entered for MicroArt at @PeterMacRes #Drosophila #flyeye

Drosophila have facilitated so many big discoveries - heredity, body patterning, circadian rhythms, innate immunity, and signalling pathways like Notch, Hedgehog, Wnt and Hippo. This facility will allow any Australian researcher to harness the genetic power of the "golden bug"

Excited to welcome the fly community to the 6th Asia Pacific Drosophila Research Conference in Cairns, Australia (23-27 July 2023). See website for more details https://t.co/Udi2zzfERT