Raffaella Di Micco
@DiMiccoLab
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Group Leader at SR-Tiget @Telethonitalia @SanraffaeleMI. Intrigued by #DNAdamage, #senescence and #stemcell biology. @ERC_research @NYSCF Robertson Investigator
Milan, Lombardy
Joined November 2011
🧨💥Our latest work is out @cellreportsmedicine! We uncovered #p38MAPK mediated #proliferation stress as the culprit of functional #impairment of #HSC during exvivo #gene #editing. p38 inhibition endows edited HSC with high #clonal #output and better preserved genome #integrity!
1/4- 📢I am thrilled to announce that @DiMiccoLab's latest study, "A p38 MAPK-ROS axis fuels proliferation stress and DNA damage during CRISPR/Cas9 gene editing in Hematopoietic Stem and Progenitor Cells" ( https://t.co/7DBiQzYhZP) is now out in @CellRepMed! Read more below👇
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We thank @martakova and @MateMaus for sharing their research with our community at yesterday's seminar! Our next seminar on Tuesday, May 6th will feature Anna Kajaste-Rudnitski and Raphael Ceccaldi. Subscribe here to receive the reminder: https://t.co/Hv6iLmjlly
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1/2 We are very excited to announce the 10th Annual ICSA Meeting, which will take places in Rome, Italy on Sept 17th-19th, 2025. Registration is now open! Visit our website for details: https://t.co/TBpuY4h9Hy We look forward to seeing you in Rome!
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📢Thrilled to chair with #MassimoLopes the next series of virtual seminars from the #GenomeIntegrityItalianNetwork. We will cover a wide range of topics spanning from #genomeinstability, #senescence, #DDR, #metabolism, #epigenome maintenance and more!Join us and spread the word👇
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Our next GiiN webinar will feature Lumír Krejčí from @muni_cz and Giulia Bastianello from @IFOMresearch. Join us on Tuesday, February 4th from 5-6 PM Rome Time for a lively discussion on genome integrity maintenance here: https://t.co/V7xPTI4YNK
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1/7 Excited to share our team’s first paper revealing how senescent cells use the GD3 ganglioside as a Senescence Immune Checkpoints (SICs) to bypass immune surveillance published in @NatureAging !
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Study shows that senescent cells literally sugar-coat themselves to avoid recognition and clearance by the immune system.
nature.com
Nature Aging - This study identifies a novel immune checkpoint in senescent cells that is linked to the ganglioside GD3 and that contributes to the evasion of immune clearance by these cells and to...
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If you are interested in senescence, ageing and age-related diseases, you can apply for Postdoc and PhD Fellowships in my lab. I´ll help you with the application! Calls close end Jan 2025! Happy holidays!!!
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1/ 🚨New paper on ecDNAs from our lab! We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! 🧵👇 https://t.co/ct2s4Rhcty
nature.com
Nature - Large extrachromosomal DNAs are engineered using a CRISPR- and Cre–loxP-based approach and shown to drive cancer in mouse models, with potential applications in determining the role...
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We are deeply thankful to all the speakers for sharing their amazing finding with the GIIN community. We also thank a lot our community for attending and stimulating fruitful discussions! We wish you all a wonderful festive season. See you all in January!
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Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations https://t.co/pKEjQMa9XX
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Now Online: Telomere function and regulation from mouse models to human ageing and disease https://t.co/bKzk20CrAY
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The last GiiN seminar of 2024 is coming soon and you don't want to miss it!! 🗣️Speakers: Ivan Marrazzi and @DiMiccoLab 🗓️When: next Tuesday (Dec 3rd), 5pm Rome - 11am NYC 📍Where: Zoom https://t.co/Aaml0MmORk
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Thrilled to share the results of our 8-year-long expedition into the Wild, the city of #NYC , the wild west of #LosAngelesCA , and more—in search of the roots of gut fungal commensalism! 🧵 https://t.co/P4vv7Ll9dp
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Are you all excited for NRMCB's newest ✨Focus✨ on #CellSenescence? We have two Reviews AND an Expert Recommendation alongside a Tools of the Trade and eight (8!!) Journal Club articles for you - one of which highlights Judith Campisi's pioneering work. https://t.co/o36fL1urVD
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Andrea Carpinteiro is the @IMMERGE_MSCA_DN Doctoral Candidate nr.6! 🎉🎉🎉 🔎Visit our website to know more:🤩 👩🔬 DC6: https://t.co/mBIHib9SWh 👩🔬Supervisor: https://t.co/VaIY1XNNdd 🇮🇹Project 6: https://t.co/KStgI2Ra2M 🔬@MSCActions 🇪🇺@EU_Commission
#DoctoralNetwork
#Immunology
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@dellaVolpe_L @bloodgenes It truly takes a village! Thanks to everyone else who contributed and to our funding agencies @ERC_Research @nyscf @HFSP @AIRC_it @Telethonitalia @MinisteroSalute. Looking forward to move these findings into innovative gene editing applications!
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This study was brilliantly led by a 💥 graduate student @dellaVolpe_L now a postdoc in Vijay Sankaran @bloodgenes lab (watch out for her outstanding work over there as well!), and brought to completion by twitterless students Fede and Roby. Perseverance and teamwork always payoff
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5. Also, p38 inhibition reduced the fraction of #micronuclei positive cells post editing, suggesting that this strategy may even reduce the #genotoxicity risk inherently associated with gene editing procedures.
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4. Temporary inhibition of #p38MAPK prolongs G1/s transition, results in less proliferation-related DNA damage, increases the #multipotency of HSCs at single cell level and increases the #engraftment of edited cells, as well as their #clonal repertoire upon transplant!
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3. If on top of this #culturestress, cells are exposed to #CRISPRCas9 and #AAV6 donor vector for #long range #HDR, HSPCs exceed a threshold of tolerable DDR and have reduced #functionality and #clonal output in primary and secondary #transplants.
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