Vadim Demichev
@DemichevLab
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Group Leader at @ChariteBerlin. Proteomics technologies and applications, DIA-NN author.
Berlin, Germany
Joined November 2018
DIA-NN 2.2.0 is released! In this update we have focused on analysis speed, which is becoming increasingly critical given the rapid transition to high-throughput proteomics. The new DIA-NN 2.2.0 Enterprise achieves up to ~1.6x median speedup on 64-cores under Windows (please see
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We have updated our Slice-PASEF preprint! It's a result of a large collaboration where we highlight its advantages for single cell proteomics. Or any other applications benefiting from sensitivity. For example, major gains for ubiquitinomics:
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Dear colleagues, would you like DIA-NN interface to change or stay similar? Would be grateful if you reply with your thoughts on this. The rationale behind the current interface is to enable quick work with multiple experiments, helped by:. - Multiple independent tabs. - All.
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RT @s_batzoglou: An exciting new paper proteomics paper describes novel insights on protein-disease mechanisms by using @seer-based MS comb….
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RT @biorxiv_biochem: Proteostasis and Unfolded Protein Response Dynamics in Human Neuron and Mouse Glia Co-culture Reveal Cell-Specific Agi….
biorxiv.org
Proteostasis, or protein homeostasis, is a tightly regulated network of cellular pathways essential for maintaining proper protein folding, trafficking, and degradation. Neurons are particularly...
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RT @slavov_n: DNA copy number variation is strongly buffered at the protein level. The buffering is much stronger than at the RNA level.….
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RT @EvosepBio: A fully automated plasma #proteomics workflow—spanning prep to #Evotip loading—using #Evosep One + #Biomek i5 is enabling sc….
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RT @nesvilab: Conventional proteomics searches struggle with many modifications and open searches may be difficult to interpret. We introdu….
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Was a great pleasure to contribute to this work DIA-NN release with support soon.
pubs.acs.org
Achieving high throughput remains a challenge in MS-based proteomics for large-scale applications. We introduce SynchroSep-MS, a novel method for parallelized, label-free proteome analysis that...
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Time and time again, it's always the same picture. DIA-NN controls FDR correctly as data reliability has been the main goal at DIA-NN's conception back in 2017 and a priority since then. Data from a very nice paper introducing the Thin-diaPASEF concept:
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Assessing FDR in proteomics using full proteome recombinant libraries: A very elegant work and a benchmarking dataset useful for many other things too. Protein FDR higher than precursor FDR likely due to paralogues - and this needs to be researched.
pubs.acs.org
Recently, deep-learning-based in silico spectral libraries have gained increasing attention. Several data-independent acquisition (DIA) software tools have integrated this feature, known as a...
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RT @DavidGomezVar: 🚀 I am thrilled to share our new publication in @NatureComms . By combining cutting-edge technology and computational in….
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RT @labs_mann: Apply today and be part of inspiring science! Deadline July 23rd! #Proteomics #Postdocs.
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RT @ypriverol: 🚀 Attention DIA/DDA #proteomics users! Whether you're using #DIANN, #MaxQuant, #quantms, or any tool that outputs mzIdentML….
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RT @EvosepBio: #Proteomics collaboration was never easier. Our multi-lab study showcase how standardized, automated workflows with #Evose….
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RT @LisaCSchweizer: I’m thrilled to share that the main work of my PhD has been published @Cancer_Cell! With @labs_mann & @ErnstLengyel, w….
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Mathias Wilhelm, @MartinFrejno from MSAID as well as Marie Locard-Paulet, @MagnusPalmblad and myself are organising a workshop on FDR control & trustworthiness of proteomics, hosted by Lorentz Center in Leiden, February 2-6, 2026. Stay tuned :)
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RT @labs_mann: Protein digestion in 200 nL on solid-phase tips: SPEC enables low-input proteomics—even with SDS/SDC at high levels. From FF….
biorxiv.org
Despite great progress, sample preparation remains an area for improvement in proteomics, particularly for low-input samples where conventional protocols lead to losses and incomplete digestion. We...
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