Jin Zhang Lab
@jinzhanglab
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We are a lab of diverse scientists developing genetically encoded biosensors for studying cell signaling at UC San Diego
UC San Diego
Joined July 2018
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Excited to share our new paper out in
@Nature
, led by our postdoc
@Dr_Yonghoon
. We report the discovery of spatially organized GPCR-mediated ERK signaling using biosensors.
#GPCR
#ERK
#biosensor
#imaging
#CellSignaling
1/6
21
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550
Trying to find a
#biosensor
for your studies, or recently publish a biosensor and want to share? Check out our biosensor database, where you can search for biosensors and submit newly published biosensors!
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Today in
@scienceadvances
, our Fluorescent Sensors Targeted to Endogenous Proteins (FluoSTEPs) take center stage! FluoSTEPs use split-GFP to recruit FRET-based biosensors to CRISPR/Cas9-tagged proteins. Local sensing without overexpression!
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Our latest biosensors have been published in
@NatureCellBio
! We developed excitation ratiometric (ExRai) biosensors for PKA, PKC, and Akt activities in live cells.
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Always reminding the lab that experiments at the microscope/FACS instrument/plate reader is your time for private conversations with the cells.
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💡Lighting Up AMPK💡🔬 We used a new AMPK biosensor to unravel mechanisms for AMPK activity at the lysosome, mitochondria, & nucleus. Out now in
@NatureComms
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330
Out in
@CellCellPress
! Our collaborative team
@jasonzxzhang
@RangamaniUCSD
& Susan Taylor discovered that cAMP induces and is sequestered into membraneless organelles formed by a regulatory subunit of PKA, important for suppressing tumorigenic signaling.
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Out today in
@nchembio
we’re excited to introduce our new ultrasensitive PKA sensor for in vivo imaging, ExRai-AKAR2! Many thanks to our wonderful team of collaborators! (1/2)
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I am honored to receive the 2022 NCI Outstanding Investigator Award and grateful for the continuous support from NCI. Congratulations to all awardees! - JZ
@UCSDPharm
@CellSignalingSD
@UCSDMedSchool
@ResearchUCSD
@theNCI
(📷 IC: NCI)
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We developed our biosensor database to accompany a new review article written by Eric Greenwald, Sohum Mehta, & Jin Zhang "Genetically encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks"
@ACSChemRev
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ALL 26 PLASMIDS from our most recent paper
@NatureCellBio
on the development of single-fluorophore kinase biosensors have passed QC and are available on
@Addgene
!
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Out in
@NatureComms
, we solidify the finding of nuclear mTORC1 activity, develop a new tool, Akt-STOPS, to suppress Akt kinase activity in location-specific manner, and reveal a new mode of regulation of mTORC1 in the nuclear compartment. (1/2)
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Make a new
#biosensor
or recently see one published? Submit to our biosensor database! Also really helpful when you think "Is there a genetically encoded biosensor for that?"
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We are very excited for our newest work in
@NatureComms
. Former postdoc & current
@UICscience
Prof Gary Mo rationally designed a new red FP for multiplexed FRET imaging of Src+Akt+Erk activities! Great collaboration with
@erin_rod_phd
!
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🔥 We have deposited many new plasmids
@Addgene
including our genetically encoded Akt inhibitor, Akt-STOPS! Check out all the plasmids available here:
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I’m truly honored to receive the 2023 BPS Carolyn Cohen Innovation Award that recognizes the work of our students, postdocs and collaborators. Many thanks for all the support! – JZ
@UCSDPharm
@CellSignalingSD
@UCSDMedSchool
@ResearchUCSD
Jin Zhang to Receive 2023 Carolyn Cohen Innovation Award
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Just in time for Christmas, our newest paper has been accepted in
@ChemicalBiology
! Lab alumni
@FabianCHertel
developed single color biosensors for co-imaging of PIP2 and PIP3 in living cells!
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I’m humbled & grateful to receive the Christian B. Anfinsen Award from the Protein Society. It’s special honor for me because Roger Tsien received this award 20 years ago. -JZ
The Protein Society is pleased to announce our 2022 Award Winners. Read the press release here:
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This is a tremendous honor. Many thanks to mentors, colleagues, collaborators as well as students and postdocs in the lab! -JZ
Congratulations to Dr. Jin Zhang, Professor and Vice Chair of Pharmacology, for the honor of being selected as one of the 2021 Fellows of the American Society of Pharmacology and Experimental Therapeutics (ASPET)
@jinzhanglab
@SilvioGutkind
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Dr. Jin Zhang has been selected as a recipient of the
@UCSanDiego
Chancellor's Award for Excellence in Postdoctoral Scholar Mentoring!🥳
@ResearchUCSD
@UCSDMedSchool
As many know, Jin is a wonderful postdoc mentor, and this award is well deserved! Congrats!!!
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Check out our flexible automated liquid-handling combined microscope (FALCOscope) system led by Dr. Eric Greenwald and
@claraposner
. It uses an open source liquid handler (
@opentrons
OT-1) to increase the throughput of fluorescent biosensor imaging🔬
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I am humbled & grateful to receive
@ASPET
Ruffolo Career Achievement Award that recognizes the work of our students, postdocs & collaborators! Many thanks for all the support -JZ
@UCSDPharm
@CellSignalingSD
@jhupharmacology
@UCSDMedSchool
@ResearchUCSD
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📢Out now, our new review: Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism by
@michellefrei13
and
@doctorsohum
in
@AnnualReviews
#Biophysics
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💡Caught in action – 3’ Phosphoinositides accumulate at the lysosome to regulate Akt! Discovered with the help of a new highly sensitive single fluorescent protein Akt biosensor 🔬 by
@dsmig_chen
, Tengqian (not on Twitter),
@yanghao_zhong
, &
@XinZhou2020
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Prepping 26 plasmids from our recent
@NatureCellBio
paper on single-fluorophore PKA, PKC, Akt, Erk, & cAMP
#biosensors
for submission to
@Addgene
!
#SharingIsCaring
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Great article in
@nature
on using
@OpenTrons_
for research feat. Zhang Lab alum Eric Greenwald! Stay tuned for more about how we use our OpenTrons liquid handlers!
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We have a new review out by Ha Neul (Skott) Lee in Current Opinion in Cell Biology about the use of biosensors and other optogenetic tools to monitor and manipulate cell signaling!
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Our latest work on Erk signaling is in
@eLife
! Spearheaded by
@KeyesDiscovery
w/
@joann_trejo
& Andre Levchenko, this work uncovers EGF-induced, Rap1-regulated plasma membrane Erk activity, temporally distinct from cytosolic & nuclear Erk activities.
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🚨Paper Alert! We used a new Ras biosensor to uncover mechanisms of Src regulation of Ras at the Golgi Apparatus, and as assay platform for Ras inhibition measurements. Out now in
@J_A_C_S
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🚨Out in
@MolecularCell
, “Molecular Determinants and Signaling Effects of PKA RIα Phase Separation” by
@a_hardy_girl
establishes that a non-canonical, active PKA R:C complex in RIα condensates sequesters PKA activity from the cytosol.
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Some of our new single-fluorophore biosensors are now available on
@Addgene
!
New plasmids from the lab of
@jinzhanglab
.
Single-
#fluorophore
#biosensors
for sensitive and multiplexed detection of signaling activities
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Today is superstar postdoc
@michellefrei13
's last day in the Zhang Lab before she goes on to start her own lab
@ETH_DCHAB
! We'll miss Michelle, but we are so so excited to see the incredible things she does in her independent career!
#newPI
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Today we received our first
#BlueFlame
award from
@Addgene
! Thank you for this award & thank you to the scientific community who finds great uses for the tools we have & are continuing to develop! You can find all our plasmids at
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Jin got the chance to catch up with Roger Tsien lab alumni - and their current and former trainees - at the Janelia FPs and Biological Sensors VII conference! Great to see
@DedeckerLab
@StPierreLab
and others tagged.
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Our latest paper is out, using genetically encoded biosensors to explore nuclear PKA. This paper is the culmination of a lot of hard work by former grad student Dr Terri Clister!
AKAP95 Organizes a Nuclear Microdomain to Control Local cAMP for Regulating Nuclear PKA
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Trying to organize a conference during the pandemic was quite an experience. After much work over the last 4 years, it’s fantastic to enjoy the Cyclic Nucleotide Phosphodiesterases
@GRC
at beautiful Les Diablerets. Many thanks to my co-chair Jos Prickaerts! -JZ
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We have a new review out in
@TrendsBiochem
! Here, we review the use of biosensors to light up signaling networks. (Behind Elsevier paywall)
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Out today in
@NatureRevCancer
Sohum Mehta
@doctorsohum
discusses how liquid-liquid phase separation shapes the biochemical activity architecture of cancer cells.
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We are having a wonderful time celebrating 40+ years of tyrosine phosphorylation at the Salk Institute! Always great to connect with the vibrant PTM and signaling community.
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We have a new review out about using
#biosensors
to study kinase signaling in cancer in
@jbiolchem
->
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Today, our very first
@UCSDBMS
grad student defended! Congrats Dr Yanghao Zhong
@yanghao_zhong
!! We are so proud of you! Dr Zhong will be deciding on where to postdoc in the next year. Congrats! 🎉🍾🎈🥳
#dragonknife
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Jin got the chance to catch up with Roger Tsien lab alumni - and their current and former trainees - at 2023 OptoRevolution: Exploring the Frontiers of Physiology with Light. Great to see Atsushi Miyawaki,
@aliceyting
,
@Campbell_FPs
,
@MichaelLinLab
and others on twitter tagged!
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Congrats to our wonderful friend & collaborator Susan Taylor, on being honored for 50 years of service in
@UCSDPharm
!!
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Wonderful afternoon for the
@UCSDPharm
Holiday Party with our Chair
@SilvioGutkind
& vice chair Jin!
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Congratulations
@ovo24279723
on getting into the
@StanfordBiosci
PhD program! 🥳 We are so proud of all Leyi has achieved in our lab over the last 3 years (not to mention her artistic talent) and are excited to see all she will accomplish!
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Today is the Roger Tsien Memorial Symposium at
#MAF2019
@UCSanDiego
! Great talks from former Tsien lab alumni, collaborators, & those inspired by Roger. A wonderful way to remember Roger!
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The lab goal was to submit 10 papers by the fall, and we've submitted 6, almost 7! Not bad! Keep an eye out for our newest work!
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We’ve started recycling packaging from
@Corning
products! It’s a free program, and Corning will take your trash! Very useful in tissue culture where we produce a lot of waste from packaging. More info ➡️
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In honor of National Postdoc Appreciation Week, we want to thank our postdocs for everything they do for the Jin Zhang lab! 🥰
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We have a new review in
@AnnualReviews
by
@Jinfan19
w/
@doctorsohum
discussing how biosensors have enabled elucidation of signaling microdomains within cells. Check it out! 👇
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Out today in
@J_A_C_S
we report dronpa-chromophore-removed fluorescence fluctuation increase by contact (DrFLINC), developed by
@garymolab
and
@WeiLin91694644
.
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We’re hiring! The
@UCSDMedSchool
Pharmacology Dept &
@UCSDChemBiochem
are looking for an Assistant Prof in Optical & Cryo-EM Imaging! Cc:
@Chemjobber
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Many congratulations to our colleague and mentor, Susan Taylor
@UCSDPharm
on winning
@ASBMB
Herbert Tabor Research Award for 2022!
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Today was the Annual Zhang Lab Design Contest! Some very exciting and creative projects were proposed. It's always very fun and inspiring to hear new ideas proposed by lab members. (And some turn into really awesome projects!)
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Congrats 🥳 Clara Posner
@claraposner
👩🏼🔬 on winning a poster prize in the
@ASPET
Division of Molecular Pharmacology Poster Competition! Clara is also participating in ASPET’s 2022-23 Mentoring Program.
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New review by
@doctorsohum
detailing our lab's efforts to develop tools for and understand the cellular biochemical activity architecture. 🔬
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Interested in endogenous tagging of proteins with biosensors? Check out our STAR Protocol for FluoSTEPs, developed by
@doctorsohum
@brian_tenner
&
@jasonzxzhang
Check out this
#protocol
for using FluoSTEPs (fluorescent sensors targeted to endogenous proteins) to measure cellular signaling events at specific microdomains, from Dr. Jin Zhang's lab
@jinzhanglab
@jasonzxzhang
@UCSDPharm
@ucsdbe
@UCSDChemBiochem
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Monday, several lab members attended the
#WhiteCoatsForBlackLives
rally
@UCSDMedSchool
to take a stand together against racial injustice & advocate for liberty and equality for everyone. We are actively learning & engaging to be
#antiracist
& encourage others to follow.
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A small reunion of Roger Tsien lab alumni at the FASEB Optical Probes conference! Great to see everyone together
@michaelzlin
,
@Campbell_FPs
,
@MillerChemBio
,
@WeinstainLab
, Jianghong Rao, and Kazuya Kikuchi!
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We have a new review out by Danielle Schmitt
@schmittwitt_
about illuminating the kinome with kinase biosensors. Check out this review highlighting in vivo imaging applications for kinase activity reporters! ⬇️⬇️⬇️
I wrote a review for Current Opinion in Chem Biol
#Omics
Issue about "Illuminating the kinome"...using
#kinase
#biosensors
in vitro and in vivo to study kinase activities! (Does not appear to be behind Elsevier pay wall!) Check it out ➡️
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Out on
@biorxivpreprint
we report ExRai AMPKAR, a new, single fluorophore excitation-ratiometric AMPK activity reporter!
I’m very excited my new manuscript from
@jinzhanglab
is out on
@biorxivpreprint
! We developed a single-FP AMPK biosensor & I used it to determine mechanisms for spatiotemporal regulation of AMPK activity at various subcellular locations.
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End of an era in the Zhang Lab! Today we said farewell to Dr Xin Zhou
@XinZhou2020
, who is joining
@EikonTX
. Xin has been in the lab for 12 yrs, & we will miss her very much. We are very excited for Xin, but we will miss her friendship & scientific creativity. Congrats Xin!
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Some of the
#RogerTsien
family tree, including academic grandchildren, were at the
#MAF2019
Roger Tsien Memorial Symposium. Amazing to see how Roger's influence has spread around the globe and inspired the next generation!
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This week the Zhang Lab will be at
#ExperimentalBiology
#ASPET2021
#ASBMB2021
. We are sharing a lot of exciting biosensor & imaging techniques this week! You can search "Jin Zhang" in the program or check out this thread for all presenters from the lab. 🧵
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Come find us during
#BPS2023
. Jin will receive the 2023 Carolyn Cohen Innovation Award📅Monday 8:00 pm. Watch her award talk . Postdoc Michelle Frei will give a talk during the Biosensor Platform📅 Monday 8:15 am Room 11AB.
#microscopy
#cellsignaling
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We’re hiring!
#chemjobs
Open Faculty Position: Assistant Professor- Pharmacology/Chemistry & Biochemistry
The focus of this search is in the broad area of Metabolism, Mitochondria Dynamics, and Human Diseases.
@SilvioGutkind
Apply:
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Out this week in
@eLife
is work spearheaded by
@brian_tenner
in a tour de force collaboration with
@jiexiao_lab
and
@RangamaniUCSD
to uncover a mode for compartmentalized phase regulation of calcium-cAMP-PKA signaling.
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🔬New paper! Rheb on standby: we found GTPase Rheb resides in the nucleus, independent of lipidation, to promote nuclear mTORC1 activity, free of gatekeeper control, out now in
@CellChemBiol
@CellPressNews
@UCSDPharm
@CellSignalingSD
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Earlier this month, Jin accepted the
@UCSanDiego
Chancellor’s Award for Excellence in Postdoctoral Mentoring! Congrats Jin!
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We’re on the cover! 📕 Check out
@NatureRevCancer
for a great review by Dr Sohum Mehta
@doctorsohum
on liquid-liquid phase separation in cancer.
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Roger Tsien's legacy is still alive & well: he is one of
@UCSanDiego
's most-cited researchers!
.
@UCSanDiego
has 52 highly cited researchers on this year's list from
@webofscience
. Congrats to all the faculty and researchers named this year!
@UofCalifornia
@UC_Newsroom
#highlycited2019
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🎉Congrats 🥳 Emily Pool, who passed her 3rd year exam and is now a PhD candidate!! (We had
@sprinkles
to celebrate). 🧁
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We are always thankful and proud of our postdocs, especially during National Postdoc Appreciation Week. Postdocs are invaluable to our lab and we are very appreciative of their hard work!
#ASBMBLovesPostdocs
#NPAW2021
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“Together, we are taking not a sledgehammer approach to treating disease, but a precise fine-tuning of cell signaling processes to correct their mis-regulation in disease, that’s the basis of precision…pharmacology, and it starts with the kind of work we’re doing here at UCSD”
Dr. Alexandra Newton, Distinguished Professor, together with Dr. Jin Zhang, Professor and Vice Chair, launched the Cell Signaling Center at UC San Diego.
@SilvioGutkind
@jinzhanglab
@NewtonLab
@CellSignalingSD
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Happy Mother’s Day! Today we celebrate all moms, including our amazing
#SciMom
#STEMmom
#mominscience
Jin!
#MothersDay
💐
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This week we had the 4th Annual Zhang Lab Beach Picnic! 🌴☀️🌴 It’s important to take time to relax during a very busy summer in the lab!
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Congratulations to Dr. Shawn Mingyuan Chen on defending his dissertation! 🥂🎉 Excited to hear about your future adventures, and congrats again on all your hard work!
#DragonKnife
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Today we had a wonderful multi-lab holiday party with the Shu Chien, Peter Wang
@peteryingxiao
, and John Shyy labs! It was especially wonderful to have Dr Chien and Mrs Chien join us for our “Collaboration Celebration”! Thank you Xin
@XinZhou2020
for organizing!
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Our twitter was silent for a while, but the lab celebrated several milestones: Yingqi successfully defended her Master thesis;
@Dr_Yonghoon
gave a talk and
@michellefrei13
won a poster award at the 2022 Roger Tsien
@CellSignalingSD
Symposium.
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A new review by
@xinzhou2020
&
@doctorsohum
about genetically encoded reporters has been selected for
@TrendsBiochem
special collection on light! Check it out here ➡️
0
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We are so thankful that the
@LoogerL
and
@NathanShaner
Labs joined our annual Zhang Lab beach outing this year! 🌴
#JointLabOuting
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Congratulations
@anne_lyons_bioe
for advancing to candidacy in Bioengineering! She blew her committee away! 🥳
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1048 followers! Thank you for all the follows! We are excited to have you along for our
#biosensor
adventures!
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Farewell to our visiting postdoc Julie Sanchez! We enjoyed hosting your visit!
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Great blog post highlighting Roger Tsein's
#NobelPrize
and our newly published single-fluorophore
#biosensors
!
Today marks the 10th anniversary of the day Roger Tsien shared the 2008
#NobelPrize
in Chemistry for the discovery and application of green fluorescent protein (GFP). Here's just one of many ways his legacy lives on:
@NatureCellBio
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Check out our new Bio-protocol paper for our recently developed anisotropy reporters!
#biosensors
Triple Fluorescence Anisotropy Reporter Imaging in Living Cells
By
@jinzhanglab
@ShenqHuey
and colleagues
@UCSDMedSchool
, a
#protocol
to measure three independent intracellular signals in living cells.
@HopkinsMedicine
@UMmedschool
#LiveImaging
@eLife
0
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Check out our latest work in
@PLOSBiology
in collaboration with Joan Heller Brown & Andrew McCulloch investigating biophasic RhoA activation from Gq receptor activation 👇
My paper is out at
@PLOSBiology
! With colleagues in
@jinzhanglab
and across UCSD, we uncovered the mechanisms and functional impact of biphasic RhoA activation from Gq-receptor activation:
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New!
@chrisqs5
&
@doctorsohum
review the broad impact of liquid-liquid phase separation (LLPS) 💧 on various cellular processes and signaling pathways, emphasizing the spatiotemporal regulation of cell signaling by LLPS and the underlying molecular mechanisms involved.
Online Now: Liquid-liquid phase separation: Orchestrating cell signaling through time and space
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Congratulations Dr.
@Jinfan19
for defending his dissertation! 🥳 We are so excited to see what you do next at the
@AdamEzraCohen
lab!
#DragonKnife
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