BiocEM Cambridge
@BiocEMcam
Followers
178
Following
75
Media
4
Statuses
70
Cryo Electron Microscopy facility at Department of Biochemistry, University of Cambridge. This is a shared account.
Joined December 2019
RT @Structure_CP: Team headed by Amanda Chaplin reports the cryo-EM structure of a DNA-PK trimer, which is a key enzyme for DNA double-stra….
0
3
0
RT @mairikilkenny: Yay, our new pre-print - many congrats to Zhan Yin @PellegriniLab! 👏👏🥳.CryoEM insights into RNA primer synthesis by the….
biorxiv.org
Eukaryotic DNA replication depends on the primosome – a complex of DNA polymerase alpha (Pol α) and primase – to initiate DNA synthesis by polymerisation of an RNA - DNA primer. Primer synthesis...
0
3
0
RT @ListerInstitute: A year after she won the Lister Prize, we catch up with @AmandaChaplin10 to learn more about her work on DNA breakage….
0
1
0
RT @PellegriniLab: Research Associate (Fixed Term) @Cambridge_Uni .Great job opportunity at @CamBiochem in the @Pel….
0
15
0
In this short article we provide evidence of these higher order assemblies, and discuss the potential formation of filament like assemblies, whilst giving our opinions of potential biological roles of such assemblies.
0
0
1
@AmandaChaplin10 @CharbonnierJea7 @CamBiochem Within this dataset we noticed the presence of a small population of particles representing higher order oligomers of DNA-PK. These are able to form via the combination of the two previously reported dimer forms.
1
0
0
@AmandaChaplin10 @CharbonnierJea7 @CamBiochem This short article reports data collected as part of the study reported previously, demonstrating the presence of alternative DNA-PK dimers
cell.com
Non-homologous end joining (NHEJ) is a key mechanism that repairs DNA-double strand breaks. Using cryo-EM, Chaplin et al. present monomeric and dimeric structures of the core NHEJ proteins and reveal...
1
0
1
Happy to share the latest work by @AmandaChaplin10 in collaboration with Kathy Meek, @CharbonnierJea7, ourselves @CamBiochem and others.
cell.com
Hardwick et al. report the cryo-EM structure of a DNA-PK trimer. This key enzyme for DNA double-strand break repair is known to adopt monomeric and alternate dimeric forms. This trimer is a combina...
1
3
12
@AmandaChaplin10 @CamBiochem @LeicStructBio @CharbonnierJea7 Also check out the related research article from @YuanHe_NWU
science.org
Three transitional states in NHEJ reveal distinct DNA-PKcs dimeric form and a likely conserved mechanism of PIKK family kinases.
0
1
1
@AmandaChaplin10 @CamBiochem @LeicStructBio This was part of a highly collaborative project including ourselves at @BiocEMcam, @CharbonnierJea7 , Kathy Meek, Patrick Calsou and others.
1
1
1
The latest work from @AmandaChaplin10, work initiated at @CamBiochem and continued at @LeicStructBio
science.org
PAXX and XLF structurally bridge alternate forms of DNA-PK dimers, explaining the redundancy between XLF and PAXX in NHEJ.
1
2
5
Congratulations Amanda - keep up the stunning work!.
Lovely to see this @BBCNews piece about @AmandaChaplin10 - one of our newest cohort of #ListerFellows!
0
2
1
RT @LeicStructBio: Midlands Cryo-EM Facility @uniofleicester has a permanent post for an Experimental Officer to assist/ train users on eve….
0
14
0
RT @lapassmore: New work from @_M_Carminati shows that dephosphorylation of RNA polymerase II by CPF (the 3' end processing machinery) prom….
0
37
0
RT @PMillerLab: The Miller lab @UnivCamPharm is looking for an enthusiastic postdoc to study antibody modulation of ion channels!. A previo….
0
7
0
RT @PMillerLab: The Miller lab is delighted to announce its first publication! Mechanisms of inhibition and activation of extrasynaptic alp….
nature.com
Nature - Cryo-electron microscopy structures are used to identify mechanisms underlying distinct features of extrasynaptic type A γ-aminobutyric acid receptors.
0
16
0
RT @vikrambabu: Happy to share our paper out in @Nature, which provides insights into activation and inhibition of a subset of extrasynapti….
nature.com
Nature - Cryo-electron microscopy structures are used to identify mechanisms underlying distinct features of extrasynaptic type A γ-aminobutyric acid receptors.
0
12
0
RT @S_Liang_: Excited to share our latest cryo-EM work @Nature of Blundell group @CamBiochem @BiocEMcam on ligand regulation of DNA-PKcs, a….
0
13
0