"genome shuffling by protoplast fusion between genetically diverse Bacillus strains generates frequent, unbiased, genome-wide recombination that mimics the effects of sexual recombination. we leveraged protoplast fusion to establish a bacterial QTL mapping platform"

Escherichia coli selection strains for growth-coupled metabolic engineering. "growth-coupled selection brings synthetic metabolism to life".

Wearing bacteriophages individually with an adhesive drug-loadable nanohelmet for treating ocular infections. "drug-loaded helmeted phages as eye drops for treating ocular infections".

Advanced research and exploration of CRISPR technology in the field of directed evolution.

Morphology engineering facilitates constructing efficient cell factories. ". review the morphology regulation mechanisms in key.industrial microorganisms to improve product yields".

Flow-induced 2D nanomaterials intercalated aligned bacterial cellulose. "For our bioreactor configuration and a growth period of 10 days, the dry cellulose sheet yield was determined to be ~7.5 mg/day.".

Engineered Lactiplantibacillus plantarum WCFS1 as a Biosensor Probe for Lung Cancer. "engineered biosensor detects proteases secreted by lung tumors".

Droplet microfluidics–based detection of rare antibiotic-resistant subpopulations in Escherichia coli from bloodstream infections. "The growth of rare resistant cells is detected by observing droplet shrinkage".

"a Cas9 targeted sequencing assay to selectively enrich and sequence genomic regions of interest". Context-Seq: CRISPR-Cas9 targeted nanopore sequencing for transmission dynamics of antimicrobial resistance.

"in vivo MutaT7 mutagenesis-mediated screening pipeline to evolve oxygen-tolerant rubisco in E. coli". In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.

Data-driven protease engineering by DNA-recording and epistasis-aware machine learning. "A recombinase-based DNA recorder that tracks protease activity against multiple substrates with high-throughput barcode sequencing".

Engineering modular enzyme assembly: .synthetic interface strategies for natural products biosynthesis applications.

Programmable genome engineering and gene modifications for plant biodesign.

"gavage with asnA-overexpressing E. coli reduced circulating levels of ammonia, creatinine and BUN, and ameliorated kidney damage". Segatella copri and gut microbial ammonia metabolism contribute to chronic kidney disease pathogenesis.

"Integrated Horizontal Gene Transfer model attempts to explain how ancient eukaryotes may have gained novel functions that allowed them to transition from extreme to more moderate habitats."

Engineering bacterial cell morphology for the design of robust cell factories. "physical, chemical and genetic approaches to alter cell size and shape".

RT @seth_shipman: New Preprint!! @alex_gonzadel accomplished a major feat on this one: ported retron recombineering, which we love so much….

Nanoencapsulation Platform for Oral Delivery of Peptides. "In vivo production of protein-loaded encapsulation in bacteria strongly stabilizes the encapsulated proteins against external stressors (acidic pH, pancreatic proteases)".

Leveraging gene editing to combat methane emissions in ruminant agriculture.