Nair @Nair Lab
@nair_lab
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#SynBio #ProteinEngineering #MetabolicEngineering #MicrobiomeEngineering lab @TuftsUniversity @TuftsEngineer @[email protected] @nair-lab.bsky.social
Medford, MA
Joined June 2018
🆕New pre-print🆕! Our first foray into 🥩cultivated meat🍖 with @KaplanLab_Tufts Kyong Lee @tuftscellag! We report that microbial lysates can be used as serum alternatives for in vitro animal cell proliferation.
biorxiv.org
Cultivated meat, the process of generating meat in vitro without sacrificing animals, is a promising alternative to the traditional practice of livestock agriculture. However, the success of this...
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This pre-print is now published! Thanks to the @tuftscellag industrial consortium for supporting this work!.@TuftsEngineer @TuftsUniversity @KaplanLab_Tufts @GoodFoodInst.See it here:
🆕New pre-print🆕! Our first foray into 🥩cultivated meat🍖 with @KaplanLab_Tufts Kyong Lee @tuftscellag! We report that microbial lysates can be used as serum alternatives for in vitro animal cell proliferation.
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RT @_SaraMolly: 🚨 We’re Hiring! 🚨. Join the Molinari Lab @UMD_BioE as a Postdoctoral Associate or Staff Scientist and help shape the future….
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RT @bioRxiv_synbio: Advancing protein display on bacterial spores through an extensive survey of coat components #….
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Finally, we show that engineered spores can be used to degrade plastic PET films. #plasticdegradation
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We compare coat proteins for surface availability and protection from inactivating agents (e.g., heat, protease), and their effect on sporulation efficiency. We also assess how different coat proteins can be co-utilized to increase display levels.
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Allelic tagging allows us to examine the activation timing and strength of promoters associated with spore coat structural proteins.
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In addition, this work investigates scarless allelic tagging of spore coat proteins using genome editing by co-transformation, analyzing factors affecting genome editing by congression.
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🚨🆕New manuscript🆕🚨from the lab! Started by @TrevorBNicks & completed by ToddChappell. This work assesses the utility of >30 spore coat proteins for #surfacedisplay - almost 3x currently described! We found that SscA is a great carrier not used before‼️.
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#ICME #microbiomeEngineering conference is ongoing now! Fantastic talks so far and excellent audience participation! @TuftsUniversity @TuftsEngineer
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RT @ChEnected: @SarahLebeer shares what she’ll be speaking about in her #ICME2024 keynote and talks about new technologies that have influe….
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There's still time to register for #ICME #MicrobiomeEngineering next week at @TuftsUniversity! Fellowships are still available to cover registration fees for trainees and asst. profs.!.
aiche.org
This conference brings together scientists across the quickly growing field of microbiome engineering. The conference integrates synthetic biology, systems biology, microbial ecology, and bioinform...
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RT @TuftsEngineer: Less than one week until the 7th International Conference on Microbiome Engineering, an event that integrates synthetic….
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RT @bioRxiv_bioE: Microbial lysates as low-cost serum replacements in cellular agriculture media formulation . #bi….
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RT @ChEnected: With a distinguished career at the intersection of veterinary medicine and biotechnology, Dr. Arvind Kumar specializes in pi….
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The work was led by post-doc Damayanti Chakravarty (Nair) & @DolginJames (Kaplan) w/ support from Pomai Yamaguchi (Lee) & many others from the Nair & Kaplan groups.
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We found that both, protein + metabolites, in VN lysate are needed to support fast growth. The cells were less stressed on VN lysate when compared to other serum alternatives and could be differentiated after several passages.
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We found that different cell lines are compatible with different microbial lysates. We were able to adapt bovine satellite cells for long-term growth on Vibrio natriegens lysate. After adaptation, their growth rate on VN lysate was similar to that on serum.
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