RT @tamumonarchlab: The lab is hiring! We have a 4-year NSF funded postdoctoral position to move the needle on the molecular bases of seaso….

Great opportunity!.

Congrats Kira!.This is so great and I’m super happy for you!!.

RT @s8shogo: Another legacy from @sassonecorsilab Special thanks for an elegant supervision from @JuleenRZierath Check this resource @Cell_….

RT @tamumonarchlab: Why working on butterflies? Amazing creatures inspiring technological innovations. Awesome PBS NOVA documentary to be a….

RT @tamumonarchlab: Hot off the press 🥳! A study from our lab reveals that the monarch butterfly light sensitive Cryptochrome 1 - but not….

RT @SatchinPanda: The Circadian #2021resolutions anyone can use. * 8 h in bed.* Wait for >1 h before first calorie. * Eat within a consis….

Finally, we investigated whether RBP motifs were enriched at the polyadenylation site of rhythmic APA isoforms. Of the 84 RBP motifs examined, almost half showed an enrichment that was rhythmic across the 24-day. 9/n

Based on these and other results, we next examined if genes could exhibit rhythms in relative 3’ UTR length. Hundreds of genes show a rhythm in 3' UTR length with shorter 3' UTR at dusk, mostly b/c distal APA isoforms preferentially peak at dawn while others peak at dusk. 8/n

To determine if changes in TF activity can regulate the expression of specific APA isoforms, we reanalyzed datasets where mice were fed only at night or arrhythmically. We found that feeding rhythms regulate rhythmic gene expression in an APA isoform-specific manner. 7/n

Because co-transcriptional loading of RBPs on nascent RNA has been proposed to regulate APA (i.e., loading facilitated by TFs), we also examined if the circadian TF Bmal1 could regulate gene expression in an APA isoform-specific manner. Short answer: it does. 6/n

So, could post-transcriptional regulation be involved?.Comparison of APA isoform rhythmic expression between nuclear and total RNA showed that, indeed, post-transcriptional events are important. 5/n

Is this because APA isoforms are expressed in different liver cell subtypes? Not really. Reanalyzing a mouse liver scRNA-Seq dataset, we found that cell subtype specific expression only explains a small fraction of the differential rhythmicity between APA isoforms. 4/n

We started with simple questions: .Are all APA isoforms of a rhythmic gene rhythmic? And could arrhythmic genes have rhythmic APA isoforms?.Using 3’ end RNA-Seq in mouse liver, we found that most genes have a combination of rhythmic and arrhythmic APA isoforms. 3/n

Work by @jbhclock @SatchinPanda and others demonstrated that 50% of genes are rhythmically expressed somewhere in the body. Yet, most genes have multiple isoforms, in part because APA generates transcripts with different 3’ ends. 2/n

Excited to share the latest story from the lab led by Ben Greenwell @b_gwell. We looked at alternative polyadenylation (APA) in the context of #circadian rhythms, and found that rhythmic gene expression is largely APA isoform specific. .1/n.

RT @TAMUScience: Congratulations to @TAMU mathematician Harold Boas, @TAMU_Biology's Paul Hardin and @TAMUChemistry's Karen Wooley, who are….

RT @tamumonarchlab: Check this out 👇.@TAMU_Biology is hiring!.3 Faculty positions in Biological Timing and 1 in Microbiology in a diverse a….

RT @tamumonarchlab: And the latest paper from the lab, led by grad student Sam Iiams, is out in PNAS! Right in time for Thanksgiving. Ph….

RT @skadener: Please RT! We have a postdoc position available at my lab for studying the role of circRNA in aging. We have exciting results….