David I Roper
@davidroper2
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Molecular & structural biochemist, AMR, bacterial cell wall, cell division & antibiotic discovery, @Warwicklifesci views my own, not UoW.
Joined December 2010
30 years on since @YSBL_York who would have thought these two old gits would find themselves in a lab together again! @jeremytame1
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Characterization of two co-regulated response regulators in Clostridioides difficile | Journal of Bacteriology
journals.asm.org
Two-component systems are nearly ubiquitous among bacteria and are one of the primary ways that bacteria respond to their environment. Atypical two-component proteins and systems are being identified...
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Analysis of essential genes in Clostridioides difficile by CRISPRi and Tn-seq | Journal of Bacteriology
journals.asm.org
Clostridioides difficile is an opportunistic pathogen for which better antibiotics are sorely needed. Most antibiotics target pathways that are essential for viability. Here, we use saturation...
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As featured at the Great Wall Symposium recently. Might have to revise my lectures on vancomycin resistance….
This antibiotic is EVG7, developed by Nathaniel Martin's lab @UniLeiden and the study was led by postdoc Elma Mons.
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This antibiotic is EVG7, developed by Nathaniel Martin's lab @UniLeiden and the study was led by postdoc Elma Mons.
science.org
Guanidino lipoglycopeptides show potent in vitro activity and in vivo efficacy against clinically relevant Gram-positive pathogens.
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In this article we describe a mechanism that regulates the progression of DNA replication when bacterial cells encounter nutrient poor conditions. Excellent work by @Surbhi_Yadav_ supported by @arnabshau @ChackoFeby @India_Alliance @IISERPune
cell.com
Surbhi et al. report that a conserved AAA+ ATPase, IncA, whose production is enhanced in nutrient-poor conditions, is required to inhibit DNA replication progression by delocalizing the β-sliding...
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The mechanistic question is why? Is it because Xeruborbactam has low activity in its own against selected PBPs in contrast to other DBOs in Pa? DBOs inhibit b-lactamases and PBP2 (elongation) in contrast to beta lactams that hit PBP3 (division).
🆕💫Efficacy of cefiderocol in combination with xeruborbactam versus taniborbactam against cefiderocol-R NDM-producing Pseudomonas aeruginosa Taniborbactam restored cefiderocol susceptibility in most isolates(20/21, 95.2%),while xeruborbactam had no effect https://t.co/QTp9gMPoIJ
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Although historically considered ineffective, β-lactam Abx have gained renewed attention. 🆕💫 Review Article:Role of β-Lactam Antibiotics in Treating Mycobacterium abscessus: From Laboratory Insights to Clinical Applications & the Case for Clinical Trials
academic.oup.com
This review highlights the evolving role of β-lactam and β-lactamase inhibitor combinations in Mycobacterium abscessus therapy, synthesizing preclinical, m
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Anyone else had a circuit board blow on a Sorval ultracentrifuge in the recent past?
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Two PhD theses submitted to day from @WarwickLifeSci Kate Willett from my lab and Becca Bailey from Phil Stansfeld’s Supervisor of one, internal examiner for another!
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New from Chris Sham at NUS: Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science
science.org
Gene redundancy complicates systematic characterization of gene function as single-gene deletions may not produce discernible phenotypes. We report dual transposon sequencing (dual Tn-seq), a...
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Lovely trip back to @UniOfYork to the carbohydrate group meeting of the @RoySocChem. Old friends and familiar places
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@InstaVoltUK 10 mile further north at Banbury sorted me out, my £40 has gone to them now as your service was none existent
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It’s been my honour and privilege to co-organise the Great Wall Symposium with Ivo Boneca for the past 14 years, roll on 2027 more news soon.
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And that’s a wrap on the Great Wall Symposium 2025! Roll on 2027, venue to be confirmed shortly …
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😳🧐🤔
A novel two-component system controls vancomycin resistance in epidemic Clostridioides difficile
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And we are off! Professor Tame of Yokohama City University starts his first experiment as part of his sabbatical thanks to an IAS fellowship here
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Molecular mechanisms underlying cefiderocol treatment failure in A. baumannii 💣Point mutations in TonB iron receptors 💣Two-component system (TCS)-mediated upregulation of the MacAB-TolC efflux pump 💣β-lactamase-mediated hydrolysis https://t.co/JncBFbciDI
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