
Brian Searle
@briansearle
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Career Scientist in Quantitative Health Sciences at @MayoClinic and @proteome_sw into immuno-oncology, proteomics, mass spectrometry & bioinformatics (he/him)
Rochester, MN
Joined October 2009
People have asked about how MAP-MS works so here's a tweetorial on @teerap16's new preprint: MAP-MS takes advantage of parallelization in a trapping MS, collecting Orbitrap MS1s with 2x the dynamic range by differentially amplifying parts of the ion beam.
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MAP-MS provides a roadmap for making better use of the ion beam. To accelerate open science, our raw data ( is already public. We've also uploaded the Exploris 480 .meth method files so you can start experimenting immediately!
searlelab.org
MAP-MS Multiple Accumulation Precursor Mass Spectrometry (MAP-MS) Description MAP-MS takes advantage of parallelization in a trapping MS. I...
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In 2013, the MacCoss lab showed a way to combine multiple groups of ions in a single spectrum to improve multiplexing with DIA to narrow isolation windows (, which morphed into the popular staggering technique. MAP-MS does the same, but for precursors.
nature.com
Nature Methods - A multiplexing strategy for data-independent acquisition (DIA)-based mass spectrometry addresses the limitation of low precursor selectivity to make DIA more practical for peptide...
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RT @OhioStatePIIO: New research in @NatureComms on rapid assay development for low input targeted #proteomics using a versatile linear ion….
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RT @MaisamHaijaMD: So proud of @V_Akshintala well deserved! We are going to do great things for the diagnosis and management of #pancreatit….
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RT @cincykidspcc: @briansearle w/ @OSUWexMed presenting his work on urine proteomics as identifiers of chronic #pancreatitis biomarkers. Hi….
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RT @MaisamHaijaMD: A wonderful lecture by @briansearle on #proteomic #applications in #childhood #pancreatitis, next phase #collaboration….
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Come join the OSU proteomics community! We're hiring a senior staff scientist at @OhioStatePIIO run by @Zihai to direct a mass spec proteomics facility to monitor immune proteins in cancer. A great opportunity for independent MS researchers! DM for details
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RT @dschweppe1: Great seeing this big collaborative effort come together with @lea_starita, @Nobu_Hamazaki, @JShendure, and led by @riddhim….
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This should be an exciting, educational lecture on ion traps! I am really looking forward to moderating audience questions for Philip during his talk tomorrow. Come join us!.
Register today for this powerful webinar organized by HUPO’s ETC – “The Past, Present and Future of Quadrupole Ion Trap Mass Spectrometers in Proteomics” Speaker Philip M. Remes, PhD – Thurs. Sept. 12 at 9 am PDT, 12 pm EDT, 5 pm CET, 12 am CST
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RT @OhioStatePIIO: Kudos to the team of @briansearle, @ArianaShannon, et al. Using a linear ion trap with 1ng total protein on column, they….
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RT @kermitmurray: (BioRxiv All) Hybrid Quadrupole Mass Filter Radial Ejection Linear Ion Trap and Intelligent Data Acquisition Enable Highl….
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