Thanks to Dirk Remus, Richard Hite, Ben Allwein.and all the co-authors. Thanks to @MSKEducation, @NIH , @theNCI , BRIA Award.@MSKCancerCenter for funding.

Unlike the bacterial hexameric ring helicases that translocate in a sequential rotary hand-over-hand mechanism, we propose CMG translocates like a 'helical inchworm', oscillating between planar and spiral states.

Importantly, the G4 traps CMG in translocation intermediate states, suggesting that CMG uses a DNA translocation mechanism that is distinct from bacterial hexameric ring helicases.

Exonuclease protection data and cryo-EM structures of the stalled CMG complexes demonstrate that the G4 is locked inside the CMG channel at the interface of the N- and C-tiers, fully folded and sequestered from G4 helicases.

We previously showed that R-loop-associated G-quadruplexes (G4s) impede replisome progression. Here, we demonstrate that a single G4 specifically in the leading strand template effectively blocks reconstituted yeast and human replisomes even in the absence of G4 stabilizers.

Thrilled to share first part of my post-doc work, published today in.@ScienceMagazine .We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.

Thanks to all the contributors for helping in putting this together.

@ben_allwein @HiteLabMSKCC @MSKEducation.

My first post-doctoral project from RemusLab, MSKCC. Cryo-EM structures of G4-stalled CMG reveal inchworm mechanism of DNA translocation

RT @MSKEducation: Meet #MSKPostdoc Sahil Batra (@artablihas) from the Remus Lab. Sahil is a structural biologist studying the molecular mec….

RT @khidkee_edu: #Didyouknow ~75% students aiming for #careers in #science want to talk to #experts BUT don’t know how to find one? Are you….

RT @Rainmaker1973: These flamingos are not fighting and that is not blood. Milk secretion in flamingos takes place in the lining of their u….

Great team work @VithaniNeha @Singh_Virender it’s awesome.

Congratulations ⁦⁦@praatik_tweets⁩ for Editor’s choice article in JBC!

It seems the diffraction is not uncommon! .1) But like many beautiful things in Life we remain oblivious to. 2) Any complexity or problem of Life can be better resolved by breaking it down into components.

Reflections of Life tree

Thanks to @VithaniNeha @praatik_tweets for constructive comments and inputs!.

Pre-print of our work now online. We attempt to rationalize the intriguing persistence of enzymatic activity in catalytic mutants of biological switches.

Breaking the cryoEM resolution barrier - back-to-back