Andy Fraser
@andy_utoronto
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you can't put a fire out with gasoline. apparently i need to say that my opinions are my opinions. so there you go.
Joined November 2014
シーケンスによる代謝物の定量法(smol-seq) 標的代謝物が結合したときに構造が変化することでバーコードDNAを遊離するアプタマーをデザイン。バーコードをシーケンスすることで代謝物量を測定。高い特異性と多重検出能力を持ち、診断や治療応用にも期待ー面白いアイデアです https://t.co/nWuPHSjnZ5
nature.com
Nature Biotechnology - Metabolites can be quantified using a combination of aptamers and DNA barcodes.
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Finally - we are now hiring multiple positions to bring this tech to reality. If you have aptamer experience or are a compbio person excited to learn how to predict aptamer-target interactions, please DM me!
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smol-seq can be run in parallel to any other sequence-based method like RNAseq, opening up easy spatial metabolomics or single-cell metabolomics. We believe that this is a true nextgen metabolomics platform that can transform how we detect metabolites and drugs.
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The SSAs are exquisitely specific — they can distinguish between very similar molecules (eg glucose vs galactose, ATP vs GTP etc) but even between stereoisomers. Since each SSA has a different barcode readout we can read out many thousands of them in parallel.
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A glucose sensor releases one barcode, a cortisol sensor releases a different barcode. By sequencing released barcodes, we can quantify which metabolites are present and can read thousands of sensors in parallel. Metabolomics thus becomes a simple problem of DNA sequencing.
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smol-seq relies on structure-switching aptamers (SSAs) to detect metabolites and give a DNA sequence output. How does this work? SSAs go through a large conformational change when they bind their target. We use that change to drive the release of a short unique DNA ‘barcode’.
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Delighted to share our paper describing a way to read levels of 100s of metabolites or drugs in parallel using DNA sequencing. This method which we call ‘smol-seq’ (Small MOLecule sequencing) harnesses the power of DNA sequencing for metabolite detection https://t.co/jxKnt9MhMX
nature.com
Nature Biotechnology - Metabolites can be quantified using a combination of aptamers and DNA barcodes.
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This needs to be unequivocally disqualifying as a Prime Minister of Canada. Full stop.
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Do you have one of those fancy interactive maps for Where to find a family doctor Open ER Deeply affordable housing unit Properly funded school A moment in time where your government wasn’t wasting money on Beer #ONpoli
#DYK our interactive map that shows the thousands of new places you can buy beer, wine, cider and coolers also shows where you can return your empty cans and bottles? Check it out to find the closest Ontario-made producer near you, as well as the most convenient place to bring
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Paying $13M for the repairs necessary to keep the Ontario Science Centre open through to 2034 would have been the equivalent of reducing the $200 pre-election handout cheques to $199.19.
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#BREAKING: A pickup truck crashes into a TTC bus at high speed at Yonge/Finch leaving 8 people injured including driver of pickup who ran off but was arrested. The 7 people hurt on the bus include the bus driver. None of the injuries are life threatening.
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This is aggressively stupid. It will accomplish nothing but to get people killed.
BREAKING: Ontario has just proposed regulations that would rip out bike lanes in Toronto on Bloor, University and Yonge: https://t.co/ug1CgvfKfr
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Canada’s Geoffrey Hinton, #AI pioneer, and @UofT computer science professor, co-winner of #NobelPrize in Physics with American John Hopfield, by @IvanSemeniuk via @globeandmail @NobelPrize https://t.co/WsXU8sre8v
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Farage, The Daily Mail, the Tory party - they all want you to believe that the racists are ordinary people that have legitimate concerns. They aren’t and they don’t - they’re thugs. The ordinary people are the ones standing against them - the uk should be proud.
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There’s a lot of lies about what is happening in UK right now, many spouted by that evil imp Musk. Well THIS is what is happening: 1000s of ordinary people marching against the tiny minority of fascists that have been terrorizing the country. THIS makes me proud of the uk 👏
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Hard to imagine a Kensington with no Ronnie’s, if you can, chuck a couple of loonies at them to help them dodge this bullet. https://t.co/Dsahsplm5D
gofundme.com
Hi from Ronnie’s local 069. after having a hell of a start to the y… Ronnie’s local 069 Nassau st needs your support for Missing money…can’t pay our tax bill
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@riken_en Finally, this was a very satisfying project as it all began as basic science: throwing drugs on worms and seeing what happened, with no disease relevance in mind. That led us to a new assay, a new drug screen, a new family of leads. Basic science opens doors!!
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