This marks my final project with ThermoFisher as I transition into new role at St. Jude. A heartfelt thank you to the ThermoFisher leadership for supporting this effort from conception to data sharing. Grateful for the incredible journey past 7 years! 🙏🌟.

@bengeliscious @BriggsGroup @MPI_Biochem @FlorentWaltz A big shoutout to @bengeliscious and his team for their massive support on this project. Without @bengeliscious, this groundbreaking effort to create a visual proteomics dataset for Chlamy would not have been possible. Thank you for making this happen! 🙌🌟#CryoET #TEAMTOMO.

@bengeliscious @BriggsGroup @MPI_Biochem Part of this data was also used in preprint from @FlorentWaltz on the native in-cell organization of the mitochondrial respiratory chain with some impressive sub-nm resolution respirasome structures .

This was a big collaborative team effort from my team at ThermoFisher, massive work from team @bengeliscious including lot of input from @bengeliscious himself and support from Juergen Plitzko and @BriggsGroup from @MPI_Biochem.

From organelle dynamics to transient interactions like mitochondrial fission & ER contact sites, this dataset captures the living architecture of a cell. Every tomogram is a story waiting to unfold. Data available on EMPIAR-11830 & CZII CryoET Data Portal.

We performed subtomogram averaging on complexes from >3 MDa to ~200 kDa, including 80S ribosomes, Rubisco, clathrin, microtubules, ATP synthase photosystem II, and nucleosomes. Most density maps achieved sub-nanometer resolution!🔬✨ #CryoET #StructuralBiology #VisualProteomics

Excited to share our new preprint and 1800+ tomograms with the community! 🎉 This is our first step towards building a visual proteomics dataset for Chlamydomonas reinhardtii. I hope the community will be able to make good use of this data!🔬#CryoET.

RT @BriggsGroup: From @ZunlongKe, collaboration with @PeacockFlu @CrollTristan @Dr_Shepp @AbhayKot @influenzal @wendybarclay11.

Thank you for the warm welcome! I’m excited to join the incredible team & continue advancing #CryoEM and #CryoET. A heartfelt thank you to my amazing team & colleagues at @thermosciEMSpec for an unforgettable journey the last 7 years. Ready to build on that momentum at St. Jude!.

RT @StJudeStructBio: We are starting our 3rd Structural and Computational Biology Symposium!. Babis Kalodimos @KalodimosLab opens the event….

RT @DimpleKaria3: Thrilled to share our paper on Sub-3Å protein structure determination at 100keV. Check out exciting results from Ceta-F a….

This was a huge team effort led by @DimpleKaria3, @AdrianKoh8, @WenYang42 , @lingboyu and all our amazing collaborators, X less Juergen Plitzko, @RaduAricescu, @BJ_Greber and @LanderLab. We are eager to hear thoughts and feedback from community.

Our results show that with the right setup, cryo-EM at 100 keV isn't just for screening samples or looking at large protein complexes; The #Tundra with Falcon C can also achieve high resolution for smaller proteins. More details in the preprint below:.

And finally, we also looked at viruses and determined the structure of Adeno-associated virus (AAV) capsids to 2.8 Å resolution with Falcon C! 🦠#Virology #GeneTherapy

Even more challenging targets like Transthyretin (55 kDa) from @LanderLab resulted in impressive 3.5 Å resolution map and flexible human haemoglobin (64 kDa) at 5.0 Å 💪 #SmallProteins #HighResolution

We looked at variety of proteins, not only large complexes but also smaller targets like Aldolase and CDK-activating kinase from @BJ_Greber group.

We achieved a 2.8 Å map of the GABAA receptor with the Falcon C, a critical target for anesthetics and neuroactive drugs. Map shows clear densities for the agonist binding pocket and the N-linked glycan on Asn149. Thank you @RaduAricescu group for samples #Membraneprotein #CryoEM

Comparing Ceta F vs. Falcon C detectors: The Ceta F gives good results with 2.6 Å for apoferritin and 3.0 Å for proteasome T20S, but the Falcon C outperformed with a 2.1 Å and 2.7 Å resolutions! This is because of the higher DQE of Falcon C. #Electroncounting, #DED

Some key findings: Different magnifications on Falcon C (0.55, 0.7, & 0.9 Å/pix) yielded reconstructions up to 2.1 Å resolution for apoferritin. Higher magnifications provided better resolution and better B-factors, but we must be careful about FOV. 🍩🍩#ResolutionMatters

Building on the work of Richard and Chris, here is our latest work showcasing the potential of the 100 keV #Tundra microscope with the Ceta F and Falcon C. We achieved high-resolution structures for a variety of protein complexes🧵.🔬#100keV #CryoEM. Movie by @lingboyu