Jeannie Lee
@XistHarvard
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X chromosome inactivation, imprinting, non-coding RNA, repetitive elements, epigenetics, 3D genome 🧬; neurodevelopmental disorders and therapeutics
Boston, Massachusetts
Joined June 2018
An evolving landscape of PRC2–RNA interactions in chromatin regulation, by Aguilar @ragmau, Rosenberg, et al. now in print! Learn the latest about how Polycomb works with RNA in epigenetic regulation: https://t.co/Ok7OXu2q6j
nature.com
Nature Reviews Molecular Cell Biology - This Perspective discusses unresolved questions about how the chromatin modifier Polycomb repressive complex 2 (PRC2) binds RNA in vivo and the functional...
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Jpx flips a bistable switch and reprograms the X-inactivation center in 3D by regulating CTCF loop formation. https://t.co/XPfse3EFhj Congrats to Hyun Jung Oh, with team members Priyojit Das, Roy Blum, Andrea Kriz, Hun-goo Lee, and Yong-woo Lee!
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If the same pipeline were applied to SPEN/SHARP, this known XIST-interactor would not meet the authors' definition of an RBP. Outstanding reconstructive work by YongWoo Lee, Priyojit Das, Barry Kesner, and Roy Blum!
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Our revised manuscript explains how Guo et al. concluded PRC2 is not an RNA binding protein. They retained unmappable reads, selectively removed read duplicates from the mappable fraction, and applied an arbitrary cutoff for PRC2 enrichment in RNA. https://t.co/SbN5bJJX5J
biorxiv.org
Using CLAP methodology, Guo et al. recently concluded that PRC2 is not an RNA binding protein (RBP). They suggest that prior findings were CLIP artifacts and argue against RNA’s direct role in PRC2...
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đź“ŁNew paper alert from the lab! Lab alumni Isabel, @n_toothacre, @claudia_lovell from @CAMBUpenn together with @PennEpiInst faculty @ericjoycelab @creminsLab investigate 3D ultrastructure of the inactive X in B cells.
cell.com
Sierra et al. investigate the transcriptional profile and ultrastructure of the inactive X chromosome in naive and in vitro-activated B cells. Their study reveals that the X chromosomes in B cells...
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With congrats to Bojan Bujisic, Hun-Goo Lee, Lilei Xu, Uri Weissbein, Carlos Rivera, and our collaborator - Ivan Topisirovic !
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A surprising discovery by @bujisic_bojan et al: The RNA component (7SL) of SRP regulates a global response to stress — controlling both transcription and translation. 7SL RNA is not just for ER translocation! Read about the findings here: https://t.co/M2d7UhkwDi
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The work resulted from a wonderful collaboration between our lab and Pilong Li's lab at Tsinghua University: Congratulations to the team -- including co-1st authors, Mingrui Ding and Danni Wang!
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A biophysical basis for the spreading behavior and limited diffusion of Xist. https://t.co/VprKO4AuoN Xist and HNRNPK form a liquid droplet that encapsulates the X-chromosome and entraps other X-inactivation factors. Xist and HNRNPK pull on each other to enhance cis-spreading!
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BREAKING NEWS The 2024 #NobelPrize in Physiology or Medicine has been awarded to Victor Ambros and Gary Ruvkun for the discovery of microRNA and its role in post-transcriptional gene regulation.
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Xciting news + Jobs alert! 🚨 After wonderful years at @CRGenomica, we are relocating beginning 2025 to @ChuSteJustine at @UMontreal. If you want to be part of @payerlab 2.0, come and join us! We are hiring at all levels – see job ads in the thread 🧵below. 👇 1/5
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Job Alert!! The UPenn Center for Women's Health and Reproductive Medicine is hiring tenure track Assistant Professors, with a special interest in endometrial biology, gyn cancers, pregnancy and reproductive processes. Apply soon:
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Additionally, re-analysis of CLAP contradicts the authors’ conclusion that CTCF and YY1 are not RBP.
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Here, we perform a re-analysis of the authors’ raw datasets and come to contrary conclusions. First, CLAP demonstrates significant PRC2 enrichment throughout the transcriptome, including in XIST’s Repeat A (RepA) motif.
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Using halo-tagged PRC2 and “CLAP” methodology, Guo et al. recently came to the conclusion that PRC2 is not an RNA binding protein (RBP). They suggested that previous findings are CLIP artifacts and argue that RNA cannot play a direct role in PRC2 regulation.
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Re-analysis of CLAP data affirms PRC2 as an RNA binding protein and XIST RNA as a target. We (YongWoo Lee, Roy Blum, Michael Rosenberg) examined the datasets of Guo et al. (PMID 38387462 - which reported that PRC2 is not an RBP) ... https://t.co/8uoRIul9eP
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What happens when Xist is deleted in B cells in female mice? Turns out aged female mice develop lupus-like disease! Check out the latest preprint from the Anguera lab, led by fabulous @claudia_lovell ! https://t.co/DIPgDppxvk
biorxiv.org
Systemic lupus erythematosus (SLE) is an autoimmune disease preferentially observed in females. X-linked gene expression in XX females is normalized to that of XY males by X-Chromosome Inactivation...
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· RNA G-quadruplex folding is developmentally specific. · Folded rG4s avidly bind PRC2 and inhibit its histone methyltransferase activity. · rG4 loss causes PRC2 hyperactivity. · rG4 results in Xist-PRC2 complexes becoming spatially entrapped, with loss of X-inactivation.
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RNA G-quadruplex folding in Xist regulates PRC2 activity on the X-chromosome. With huge congrats to Yong Woo Lee! Check it out: https://t.co/3ONXfIDykr
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