What an Oocyte day!! Many thanks to organizers @DDrutovic @AndrejSusor @SvobodaLab. The day full of science ended with a late night football match. Nice to meet @LastChrisThomas @zaffagg3 @CavazzaLab. Save travel everyone!

Excited and borderline anxious to be among such bright scientists!

📢 #Job Alert! #postdoc position available in my lab! You have experience in #cell #biology / #biochemistry and you wish to learn a novel way to organise the endolysosomal system? Then look no further! 👇🏼 We have a fully funded position for up to 5 years to study the regulation

While many lessons are still to be learned, we hope this work will contribute to a deeper understanding of embryo development and better outcomes of IVF-based treatments. https://t.co/xPrgPOi6Ej @Science_MEDMUNI @MUNI_Science

If you would love to know more check out the full version of our paper and don’t forget to check early papers of @ZernickaGoetz , and recent works of @CavazzaLab , @CurrieCerys and Yuki Ono.

It is likely that, spindle defocusing caused by centrosome misplacement is the basis of non-perpendicular division that we observe in our dataset. https://t.co/QyE3n7f91A

During the revision of this manuscript study by Ono et al. employed live dyes based approach to label zygotic spindle in human embryos. Their elegant imaging showed that defocusing of spindle poles leads to chromosome missegregtions.

Finally, it is tempting to speculate about the position of centrosomes during the first cell cycle and cell division. However, we were not able to address this model experimentally due to ethical constraints.

Observed link between zygotic spindle orientation and nuclear status of two cell stage embryos was even more pronounced in embryos derived from eggs of women of advanced maternal age.

Importantly 50% (!!!) of embryos that resulted in a live birth were characterised by presence of multiple nuclei at the two cell stage. This observation indicates that multinucleation at the two cell stage should not be seen as a deselecting marker.

We noticed that embryos with non-perpendicular division and unclustered nucleoli produced two-cell embryos with multiple nuclei per blastomere.

We saw that embryos with non-perpendicular division were not successful at nucleoli clustering. We followed the development of embryos to the two-cell stage and assessed the nuclear status of resulting blastomeres.

We next asked ourselves whereas the orientation of zygotic division is linked to the clustering of PN content as previously reported by @CavazzaLab, @SchuhLab. Clustering of chromatin and associated nucleoli is critical for correct cell division.

We first looked into geometry of PN arrangement in human zygotes. However, we didn’t see any preference in PN position regarding to polar bodies. On the other hand we observed that the zygotic division tented to occur at 90 degrees to juxtaposed pronuclei.

Here we analysed time lapse recording of human embryos, which resulted in live birth and sibling embryos with abnormal development.

Multiple scoring systems has been proposed, like pronuclear bodies count and alignment or angle between pronuclei and polar bodies. However, classical embryo morphology assessment is based on a limited number of observations and lacks the fourth dimension.

Embryo morphology assessment is a routine procedure in the clinical IVF aimed to evaluate developmental potential of individual embryos. Zygote assessment is the first time-point where the morphology is assessed during fert check.

I am happy to share the first paper from my PhD journey, published today. A big thank you goes to @holubcova_lab and everyone involved in this project. Here, we show how clinical time-lapse recordings can be used to address biological questions behind early human development.

🚨 Job Alert for Postdoc! We are looking for a passionate and motivated postdoc to join our team and work on the mechanism and functions of 🐄 embryo cell size regulation. Check the image for more details on the position, the project, and how to apply. (1/2⬇️)

Officially out in @NatMetabolism📖: our latest work showing changes how cells acquire amino acids during early development. Congratulations to Pavlina! https://t.co/D0aIOrp2GX @SKI_CellBiology