Out now in @ScienceAdvances 👇 A great team effort, spearheaded by Angeliki, showing that promoter and enhancer dynamics become homogenized on transcriptional activation, and that loop extrusion and transcription compete to put brakes on chromatin.

In brief, we will use machine learning to determine the "grammar" of TAD boundaries, and will test our hypotheses in a functional assay, incorporating both 4C/Hi-C and live microscopy.

Calling all talented Indian students looking for a PhD! I have a position open from CEFIPRA, in collaboration with Profs @rsidd120 and Leelavarti Narlikar, details here:

Our new review is now online: everything you wanted to know (at least what is known in this nascent field) about promoter-enhancer locus dynamics but were afraid to ask. Great work @_mar_na!

RT @mpi_ie: Excitement on both sides of the Rhine. The 1st #TriRhena Gene Regulation Club in 2025 will take place on Feb. 5th at @IGBMC in….

The perfect addition to our Christmas tree: an ES cell and a cancer cell, knitted by the super talented @cathie_erb

RT @mitchell_lab: Career achievement unlocked - two publications come out on the same day!. Great collaboration with Tom Sexton. https://t.c….

Massive thanks to @kerstinbys @tinekelenstra @mitchell_lab @AkisPapantonis @molina_lab @EviSoutoglou and team members, and @NeoVirTech for their valuable contributions.

Quite rehashed from the original bioRxiv: but the message is inherently the same.

Excited to present Angeliki's work at @BabrahamInst today. Strange to be back at where my scientific career started. 20 years ago! Looking forward to great discussions with @stefanschoenfe1 @Peter_Fraser1 and more.

Loving the occasional old school tech of #cshlnucleus

It starts: #cshlnucleus.Delighted and honoured to present Angeliki's work tomorrow. Will be happy to hear all your feedback!.

Excited to meet everyone at @mitchell_lab today and present at the CSB seminar in University of Toronto. My first time in Canada: what a beautiful and friendly place!.

RT @NAR_Open: Breakthrough**Gene-to-gene coordinated regulation of transcription and alternative splicing by 3D chromatin remodeling upon N….

Thanks to @ERC_Research and ANR for funding, as well as the great facilities at @IGBMC and ENS Lyon.

This is a heavily context-dependent phenomenon, considering the numbers of counter-examples in this and many other studies. But it is important to realise that CTCF-mediated loop extrusion blocking is not the only means to regulated chromosome folding.

Most importantly, ectopic activation of single genes is sufficient to remodel TADs in embryonic stem cells, showing that transcription CAN directly remodel chromatin architecture.

In agreement with other studies, intra-gene compaction indeed correlates with hallmarks of active transcription, not just within a cell type, but when following CHANGES during differentiation.

And some activated genes formed their own induced TADs, not really linked to new TAD borders but more increased intra-gene compaction.

Interesting exceptions: a TAD border tightly fell at a paused PolII site in early thymocytes, with "widening" of the border at later stages corresponding with full PolII tracking into gene.