Automated Cell-free Protein Synthesis for Distributed Biomanufacturing

FreeBindCraft brings open-source protein design to everyone. By replacing PyRosetta with MIT/BSD-licensed alternatives, BindCraft has been freed from its licensing constraints. Available now on Ariax! Read our announcement at

Why PETase for our tournament? In 2024, the world made about 30 million tonnes of PET plastic, most from fossil fuels. PETase can degrade PET, but isn’t ready for industrial-scale waste. The challenge: design an improved variant that can change that. Register by Oct 17 👇

Evaluating zero-shot prediction of protein design success @grocklin https://t.co/jPaB7e5C9E

God Biotech. Consumer biotech is barely starting to sink in. Bringing capitalized biology into the hands of billions in the form of weight loss pills, genetic tests, and soon, lab-grown designer babies. You and I can't even conceive of being biotech creators. The moral duty of

Staining our Forma Stool in black using a water-based black stain. Two coats and then finishing with a satin clear top coat. The results are beautiful.

Great talk by @sami_chaaban @Carter_Lab about the proper use and caveats of AlphaFold2/3 predictions, highly recommended to watch

How can we make medicines more accessible? Cell-free manufacturing offers a way. New work from @PardeeLab shows multi-site implementation of cell-free systems across continents, countries, and labs... https://t.co/QfOTRxAzwh

International Multi-site Implementation of Local Cell-Free Protein Biomanufacturing to Advance Health and Research Equity

"...we establish an all-cell-free viral cycle where T7 phages infect synthetic cells, equipped with lipopolysaccharides on the outer leaflet of the lipid membrane while encapsulating a cell-free gene expression system." Looks useful; an in vitro system to study viral infections.

Reading about discoveries in synthetic biology really puts things in perspective. We now have: CRISPR gene editing (2012) Sub $0.10/bp DNA (~2016) Benchtop sequencers (2015) Ultra-high-yielding cell-free systems (~2013) High accuracy structure prediction (~2021)

@TwistBioscience We LOVE @TwistBioscience btw! (and potatoes). They make our workflows better and faster!!

Ordering plasmids from @TwistBioscience like: I'll just throw one in there, even if I don’t need it. By the time it’s done, who knows? 🤷‍♂️😂

Mixtures of engineered bacteria were able to: - Identify if a number is prime - Check if a letter in a string is a vowel - Determine the max number of pieces of a pie obtained from n straight cuts. Answers are printed by expressing fluorescent proteins in different patterns.

This robot is controlled by a fungus. The fungus' mycelia senses the environment and emits "action potential–like spiking voltages" to control the motors and valves.

In today's strain engineering pile (plate by @pipette_jockey )

Today in @Nature we present the results of our exploration of the soluble protein universe and the design of functional soluble membrane proteins with @CasperGoverde @GoldbachNico @befcorreia 1/6 https://t.co/Dakeh8Hyw7

Long form grant applications don't impact proposal rankings by panelists when compared to one-paragraph summaries (with CVs included). https://t.co/SilS1M9Cjv

AF3 server is LIVE! Just tried predicting complex with almost 5K amino acids. TIP: you need to click "continue with google" to access the server (otherwise the "server" is grayed out). https://t.co/SeLHl8kIDP

I am pleased to announce the conclusion of the Protein Design Games Winter 2024, hosted by RosettaCon and sponsored by Liberum Biotech! Five teams competed head to head to design the best TEV protease, only one came out on top!!! https://t.co/3r0baaKiuM https://t.co/52uyMahBcS

Can hackathons be a thing in biology? We say yes! https://t.co/WrlFSm4rjs