Filippo Caschera
@Stjle
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Synthetic Biology, Artificial Cells, Amphiphile Systems, Machine Learning, Cell-Free Protein Synthesis: Italy, Denmark, Japan, USA, France, Germany, UK.
Rome, Lazio
Joined April 2010
Multiplexed bacteriocin synthesis to combat and prevent antimicrobial resistance
nature.com
Communications Biology - Cell-free expression with engineered DNA enables rapid production of bacteriocin cocktails, overcoming antimicrobial resistance and clearing pathogenic infections in animal...
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Negative design enables cell-free expression and folding of designed transmembrane β-Barrels https://t.co/Lik3Fd1Obs
#biorxiv_biophys
biorxiv.org
De novo design of membrane proteins (MPs) is a rapidly growing field with transformative potential for synthetic biology. Yet, progress has lagged behind that of soluble proteins, largely due to...
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High-throughput peptide-centric local stability assay extends protein–ligand identification to membrane proteins, tissues and bacteria
nature.com
Nature Structural & Molecular Biology - Li et al. further develop a high-throughput peptide-centric local stability assay that speeds up sample preparation 100-fold and extends...
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An AI-driven workflow for the accelerated optimization of cell-free protein synthesis: iScience
cell.com
Protein; Metabolic Engineering; Synthetic biology
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In a wonderful collaboration with Farren Isaacs and Jill Banfield, Kosuke Seki led work to discover a new orthogonal translation system from metagenomic data that suppresses the UGA codon, and then demonstrated we could evolve it. https://t.co/2yaRJqbxN6
biorxiv.org
Genetic code expansion with non-canonical amino acids (ncAAs) opens new opportunities for the function and design of proteins by broadening their chemical repertoire. Unfortunately, ncAA incorporat...
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How far are we from "protein printers" that enable anyone to make a protein of any sequence on-demand in a couple hours? I'm imagining a box that takes a protein design from a computer and then physically makes it on a bench or desk. This isn't yet feasible. It's still
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Tumor-on-Chip as a Personalised Platform for Rapid Drug-Testing in Breast Cancer
biorxiv.org
Breast cancer (BC) remains one of the most common malignancies worldwide and continues to pose major therapeutic challenges, emphasizing the need for functional models that can inform fast treatment...
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Synthetic cells for phage therapy: a perspective
frontiersin.org
A synthetic cell is a membrane-bound vesicle that encapsulates cell-free transcription/translation (TXTL) systems. It represents a transformative platform fo...
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The 60-year evolution of lipid nanoparticles for nucleic acid delivery
nature.com
Nature Reviews Drug Discovery - Lipid nanoparticle-based systems are increasingly being utilized for the delivery of nucleic acid-based vaccines and therapeutics. In this Perspective, Cullis and...
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We are thrilled to announce that our new paper is now out from #JCR. We discovered hydrophobic peptides that lead antibody onto liposome membrane during cell-free protein synthesis, enabling to produce liposomes capable of site-specific delivery in hours. https://t.co/rb3S42mCik
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@Stjle Automated cell-free protein synthesis is indeed a promising advancement, particularly for distributed biomanufacturing. It's fascinating how this approach can aid in decentralizing production, potentially leading to rapid response capabilities in medicine. Have you considered the
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Cell-Free-Based Thermophilic Biocatalyst for the Synthesis of Amino Acids from One-Carbon Feedstocks https://t.co/G47eZxL48j
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Quantitative agent-based models: a promising alternative for macroeconomics
academic.oup.com
Abstract. Agent-based models (ABMs) are dynamic computer simulations that abandon utility maximization and instead assume that agents are boundedly rationa
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My first manuscript as an independent PI, and my first single-author research article, is now published in @PNASNews. It's an attempt to contribute to the dialogue on generalizability in structure-based protein-small molecule affinity prediction with neural networks.
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Accelerating protein design by scaling experimental characterization
biorxiv.org
Recent advances in de novo protein design have greatly outpaced standard protein biochemistry workflows, and experimental testing has been a bottleneck in the validation of new designs and methodol...
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Automated Cell-free Protein Synthesis for Distributed Biomanufacturing
medrxiv.org
Recombinant proteins are fundamental to modern medicine, enabling research, diagnostics, and drug discovery, yet their access is often restricted by centralized manufacturing and cold-chain distrib...
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Now online! De novo designed voltage-gated anion channels suppress neuron firing
cell.com
This study reports the successful de novo design of voltage-gated anion channels that are distinct from native channels. They exhibit voltage-dependent ion conductance and are capable of being tuned...
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Assembly and cell-free expression of a partial genome for the synthetic cell https://t.co/evcjbLQt4K
#biorxiv_synbio
biorxiv.org
The de novo design and assembly of a DNA genome represents an important milestone toward the construction of a minimal synthetic cell. The genome must contain all the instructions to enable primary...
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Pushbutton-activated microfluidic cell-free biosensor for multiplexed pathogen detection - Lab on a Chip
pubs.rsc.org
In this paper, we have developed a novel cell-free biosensor based on a multiplexed pushbutton-activated microfluidic device (mPAMD) that enables simultaneous detection of multiple 16S rRNAs of...
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