Out now! Probe‑dependent Proximity Profiling (ProPPr) of Phospho‑Tau‑Associated Proteomes across Tauopathies by @DMorderer and @NeuroWren. Great collaboration with @DrNeuroChic and Dr. Dennis Dickson! @MayoClinicNeuro @MolNeuro @cziscience @NIHAging.

Our study provides a first comprehensive overview of pTau-associated proteomes across tauopathies and uncovered several important findings. It is also a starting point for further ProPPr projects, where we can put to good use everything that we have learned during this journey.

Our analysis demonstrated that infiltration of APs with FTL-positive microglia is a characteristic feature of CBD pathology. The observed complex co-localization patterns underscore the importance of using fluorescence microscopy to follow up on proximity proteomics findings.

To discover proteins with differential association with pTau lesions between AD, CBD, PiD and PSP, we performed statistical comparison of protein abundances. We found a strong pTau-association of ferritin light chain (FTL) for CBD but in what cell type or what pTau lesion?

We then selected a set of common or tauopathy-specific enriched proteins for further analysis with immunofluorescence and quantitative analysis. Represented in all tauopathies was the core retromer complex protein VPS35 that we found sequestered in several types of pTau lesions.

We used AT8 phospho-tau (pTau) antibody as a probe to determine the composition of disease-specific pTau-associated proteomes in post-mortem frontal cortices of four major tauopathies in situ. We report 1,317 pTau associated proteins, with 409 being new.

@DMorderer @NeuroWren @DrNeuroChic @MayoClinicNeuro @MolNeuro @cziscience @NIHAging To identify proteins that associate with tau pathology in these tauopathies, we developed an approach for in situ proximity labeling and isolation of aggregate-associated proteins using glass slides with formalin-fixed paraffin-embedded (FFPE) human brain tissue, termed ProPPr.

@DMorderer @NeuroWren @DrNeuroChic @MayoClinicNeuro @MolNeuro @cziscience @NIHAging Here we addressed the great disease-specific morphologic and biochemical diversity of tau aggregates in Alzheimer’s disease (AD), corticobasal degeneration (CBD), Pick’s disease (PiD), and progressive supranuclear palsy (PSP), that remained to be explored with unbiased methods.

RT @JMTali: Excited to share our latest work! We asked if and how TDP-43 regulates RNA transport in neurons and if this is misregulated in….

RT @labs_mann: Introducing mipDVP (multiplexed imaging-powered Deep Visual Proteomics)! Using 22-marker imaging to profile proteomes across….

RT @Katz_Lab: If Nature and Science journals both agree on this, surely there is at least a 60% chance it is true.

Looks like PPS Silent Surfactant, Progenta anionic acid labile surfactant (AALS) I, AALS II, cationic acid labile surfactant (CALS) I, and CALS II are not commercially available anymore.

Can someone please point us to a source for mass spectrometry compatible surfactants less pricey than Invitrosol, ProteaseMax, and RapiGest SF? DDM did not work that well in our hands for FFPE tissue extraction and proteomics.

RT @JMTali: Excited to share our latest work! We developed a new method for studying RNA localization via proximity labeling: OINC-seq! In….

RT @aliceyting: APEX peroxidase is a versatile tool for proximity labeling and electron microscopy (EM) but its requirement for toxic H2O2….

RT @Schliekerlab: introducing the folding phase-check out our new opinion article in which we propose a protein folding function of the nuc….

RT @LiveLikeLou4: We are coming off three days of outstanding discussions and collaboration to advance pre-clinical ALS research. Our bienn….

RT @biorxivpreprint: Heteromeric amyloid filaments of ANXA11 and TDP-43 in FTLD-TDP Type C #bioRxiv.