Evan Reid
@ReidLab_HSP
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Prof of Neurogenetics and Molecular Neurobiology, Cambridge Uni. NHS Clinical Geneticist. Study membrane traffic and axonal degeneration in HSP. Personal views.
CIMR, University of Cambridge
Joined October 2010
Our first cohort of students on our MPhil course 'Molecular Mechanism of Human Disease' are settling in so it's time to open applications for next year! Deadline is 3rd December and you can find all the details here 👇
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Out at @naturemethods just now! https://t.co/SAiolzlnBy We present #SN2N, a flagship level unsupervised denoising solution. It’s fully competitive with supervised learning without needing large training-set and clean ground-truth, using 1 noisy frame for training. (1/n)
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Congratulations! @SerenaNikZainal
Congratulations to Professor Nik-Zainal for winning the 2024 ESMO Award for Translational Research | Oncology Awards: https://t.co/LkoT2xPt6F
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Edward, Lena and I are hosting an ER session at #cellbio2024 #ASCBEMBO this year. It will be…awesome! 😂. Submit your abstracts and come join us!
🚨With @AbellJonny & @Kosloverlab we are excited to organise “The Endoplasmic Reticulum Structure and Function Across Cell Types” session at #cellbio2024 #ASCBEMBO! If you are interested in ER, its cellular functions, send us your abstracts by Aug 6🔬 https://t.co/6eq3cyGlVZ
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Hopefully this provides more insights into how protrudin influences polarised membrane traffic and functions in neurite extension & axonal regeneration (the latter shown by another ex-colleague @ves_petrova and current colleagues @BartNieuwenhu1s, @RichardEvaLab, James Fawcett)
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This work was done in the fantastic @TheCIMR and helped by the advice and expertise of many wonderful colleagues there. Thanks go to them!
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Protrudin sits within an interacting network of proteins implicated in endosomal tubule fission, meaning it is ideally placed to co-ordinate elements involved in this process.
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- finally, the endosomal tubule fission process is enriched for proteins involved in motor neuron disorders, and we show that human cortical neurons lacking protrudin or KIF5 develop abnormal endosomal tubulation, consistent with a fission defect.
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-this means the process is mechanistically (and likely spatially) distinct from protrudin's known role in late endosome motility, which requires FYCO1 - results are consistent with a model where protrudin facilitates a tug-of-war between KIF5 and dynein to fission the tubules
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- these phenotypes can be rescued by wild-type protrudin, but not by forms that cannot bind VAPs at the ER or endosomal phosphoinositides, or by forms unable to bind KIF5 - KIF5 depletion causes a similar phenotype, but depletion of FYCO1 does not
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In brief: - Protrudin acts at ER-endosome contacts to promote fission of tubules from sorting endosomes - lack of protrudin leads to defective tubule fission and blocked traffic of the mannose 6-phosphate receptor
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This is mostly the work of @juliakleniuk, a wonderful student who has just finished her PhD in my lab, with a bit of help from Aish Nadadhur, Eliska Zlamalova, Emily Wolfenden (a medical student with a great future!) and Catherine Rodger.
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Excited to unveil our latest work: Protrudin acts at ER-endosome contacts to promote KIF5-mediated endosomal fission and endosome-to-Golgi transport
biorxiv.org
Protrudin binds ER-localised VAPs and endosomal phosphoinositides to form ER-endosome contacts that promote endosomal tubule fission and endosome-to-Golgi traffic. Protrudin recruits KIF5 to provide...
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The latest preprint from our lab. Congratulations to the first author @EdenJess and thank you to all our wonderful collaborators. @TheCIMR @CellBiol_MRCLMB @JLS_Lab
RABGAP1 acts as a sensor to facilitate sorting and processing of amyloid precursor protein https://t.co/vIjEC7gA5o
#biorxiv_cellbio
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It is well worth reading the paper that Cahir links to here. And stop using n= number of cells please.
"Failure to recognize that cells are not independent replicates when testing for differences in means with t tests or ANOVA causes pseudoreplication, an extremely common and serious error that artificially decreases P values and can create false positive effects."
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Worth a look if you're interested in how #ERshape can affect other cellular processes. Also gives some insights into the pathogenesis of #hereditaryspasticparaplegia #HSP in human neurons
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Our latest thing: Atlastin-1 regulates endosomal tubulation and lysosomal proteolysis in...
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Looking forward to seeing this blog site from @Lab_Coleman develop. Essential reading for all scientists, but especially useful for those pre-tenure: Science Without Anguish - My Site
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