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Pilhofer Lab

@PilhoferLab

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#cryoET, #cryoFIB and more to uncover the secrets of cell-cell interactions mediated by Contractile Injection Systems and other pathways. - run by lab members -

Zurich, Switzerland
Joined July 2017
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@PilhoferLab
Pilhofer Lab
9 months
Captain Aureispira - a bacterial pirate ship🦜🏴‍☠️🪝- boarded @ScienceMagazine. Check out the manuscript here: and have a look on our extended thread below ⬇️We provide new insights on bacterial predation from molecules ⚛️to ocean samples 🌊
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@PilhoferLab
Pilhofer Lab
1 year
Bacteria can be pirates too. 🏴‍☠️ Let us introduce you to Captain Aureispira ⛵️ A bacterial pirate ship loaded with canons 💉 and grappling hooks 🪝🌴predating on other bacteria 🦠 Check the preprint: #predation #T6SS #T9SS #cryoEM #cryoET #teamtomo 🧵1/6
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@PilhoferLab
Pilhofer Lab
8 months
RT @ETH_DBIOL: In a collaboration between the @PilhoferLab and the Albers Lab from the University of Freiburg, the researchers show that h….
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@PilhoferLab
Pilhofer Lab
8 months
Bye bye X/Twitter!.Please come visit us on Bluesky @pilhoferlab.bsky.social for future lab news, including a new paper on an Archaeal Type 6 Secretion System!.
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@PilhoferLab
Pilhofer Lab
9 months
RT @ETH_en: Researchers at ETH Zurich have analysed down to the smallest detail the unusual arsenal of weapons that a predatory marine bact….
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@PilhoferLab
Pilhofer Lab
9 months
The peer-reviewed version of the manuscript can be found here: 🙏 We thank the reviewers for their constructive feedback!.
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@PilhoferLab
Pilhofer Lab
9 months
Fly-fishing for flagella. Check out the lovely perspective by Andrew Lovering and @SimonCaulton 🙏🙏 about our findings on bacterial predation via ixotrophy.
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@PilhoferLab
Pilhofer Lab
1 year
Read the preprint here: to see how we used multidisciplinary approaches like #genetics 🧬, #cryoET ❄️🔬, single-particle #cryoEM, #lightmicroscopy 💡, and functional assays to unravel the secrets of this fascinating antibiotic-producing bacterium. 💊 🧵2/2.
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@PilhoferLab
Pilhofer Lab
1 year
Exciting news!🚀 We have a new follow-up on the cytoplasmic Contractile Injection System from #Streptomyces!💉@casub1, in a wonderful collaboration with @JWSallmenII & @SusanSchlimpert, unveils the critical role of CisA in Streptomyces CIS functionality. ☠️🧵 1/2
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@PilhoferLab
Pilhofer Lab
1 year
An accompanying paper from the Matos lab in collaboration 👯‍♀️👯 with us and the @pedrobeltrao lab and Aebersold lab at @Dev_Cell unveils coordinated waves 🌊🌊🌊 of protein expression and phosphorylation in yeast gametogenesis.
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@PilhoferLab
Pilhofer Lab
1 year
What are the functions of these filaments? Structural characterization and functional assays suggest that Acs1 filaments facilitates the recovery of chronologically aged spores 😴and, more generally, the cell cycle re-entry of starved cells. 🥳🧵(5/5)
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@PilhoferLab
Pilhofer Lab
1 year
Using #FilamentID, we found that the conserved aldehyde dehydrogenase 4 (Ald4) forms filaments in the mitochondria, whereas acetyl-CoA synthetase 1 (Acs1) polymerizes into nuclear and cytoplasmic filaments 🔍 🧵(4/5)
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@PilhoferLab
Pilhofer Lab
1 year
To find out what they are, we combined cryoET with “targeted” single particle cryoEM (we call it #FilamentID) to image partially lysed and spread cells or organelles and identified two different metabolic enzyme filaments. 🤯 🧵(3/5)
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@PilhoferLab
Pilhofer Lab
1 year
We saw that meiotic budding yeast cells are full of different filaments. 🧐(some of you might have also seen them in your yeast tomograms) and these filaments seemed to be specific to meiosis. 🧵(2/5)
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@PilhoferLab
Pilhofer Lab
1 year
To #TeamTomo ❄️🔬: Do you also sometimes see strange filaments in your #cryoET data and don’t know what they are? 🤔 Try #FilamentID! 🪄🤓 Check out our new paper at @CellCellPress by @HugenerJannik and @xujw_thu together with the Matos lab 🧵(1/5)
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@PilhoferLab
Pilhofer Lab
1 year
Free DPANNs are motile, but ectosymbiotic cells are linked with the host via an elaborate attachment organelle, making them the potentially first microorganisms using a straw 🥤 Also have a look at the beautifully complementing story from @DebnathGhosal:
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@PilhoferLab
Pilhofer Lab
1 year
#cryoET as discovery tool ❄️🔬: DPANN archaea are enigmatic, hard to cultivate, and rely on an ectosymbiotic lifestyle. Check out our new paper @ISMEJournal from Vasil Gaisin in collaboration with @Archaellum where we describe two DPANN lifecycle stages:
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@PilhoferLab
Pilhofer Lab
1 year
Have you ever had nightmares of killer archaea? 🔪☠️😱 stop dreaming! Have a look at our latest preprint on how Halogeometricum borinquense uses it type 6 secretion system to kill Haloferax volcanii:
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@PilhoferLab
Pilhofer Lab
1 year
RT @PilhoferLab: It's out! It's out! 📢The bacterial Tc Toxins are out😱☠️ (and our paper 📜😉)! But how did these huge complexes get out? Whe….
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@PilhoferLab
Pilhofer Lab
1 year
RT @NatureMicrobiol: See also the associated paper:. Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell….
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@PilhoferLab
Pilhofer Lab
1 year
Congrats to @miki_hannah and to all collaboration partners.
@NatureMicrobiol
Nature Microbiology
1 year
OUT NOW 📢: Stepwise assembly and release of Tc toxins from Yersinia entomophaga. By Miki Feldmüller, Martin Pilhofer & colleagues. @mikihannah @PilhoferLab @ETH_en #cryoET #teamtomo.
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