RT @GeneticsGSA: New research in #GENETICS from @NMatange and colleagues showcases canonical and non-canonical strategies employed by #Ecol….

RT @eLife: Not all persister bacteria are dormant! 🦠. Single-cell imaging of over a million #EColi shows that many antibiotic-resistant per….

Thanks to @GeneticsGSA for excellent and rigorous reviews. Thanks to the @India_Alliance for generous funding.

Digging deeper led us to identify some interesting links between proton/PMF homeostasis and antibiotic susceptibility. Kudos to Chinmaya @imcjena16 and his trusty accomplice @DeolankarSoham this one was no less than a Holmes mystery!.

Chinmaya exposed E.coli to low levels of an environmentally relevant antibiotic, Spectinomycin. He asked if resistance is the only kind of adaptation possible. Turns out there are mutations that allow low drug specific adaptations but don’t alter antibiotic MIC values.

RT @Saritapuri24: This is indeed a serious concern. Please intervene @IndiaDST @DrJitendraSingh @PMOIndia.

RT @AdhesionLab: Special Call for PhD intake in Biology @IISERPune .This is for Dec 2024 CSIR-UGC-NET qualified candidates, whose results w….

RT @ChabaRachna: We have 2-3 Ph.D. positions in @IISERMohali. Students interested in understanding how bacteria sen….

RT @eLife: 1/ The Trump administration is throwing science and scientists into turmoil – how should the scientific community respond?. Read….

RT @evoimmunoimroze: My lab will have an open position for a PhD student to work on the micro- and macro-evolutionary dynamics of immunity….

Many thanks to generous support from the @India_Alliance that made doing this kind of work possible. Also, greatly appreciative of @eLife and its new model is making the peer review process enriching.

In this work we look at the interplay between gene duplication and point mutations during the evolution of AMR. We discover that maintenance of duplicate gene copies is driven by expression demand generated by antibiotic as well as the action of proteases like Lon in E.coli.

Hot off the press, the VOR of our latest paper is now available at @eLife: Proteostasis modulates gene dosage evolution in antibiotic-resistant bacteria

@ACSPublications @SandhyaGansan.

A plug for a special issue of ACS Infectious Diseases on Research in the Global South that I am co-editing. Call for Papers: Infectious Diseases Research in the Global South: Treatments and Treatment Failures | ACS Infectious Diseases

RT @MayurikaLahiri: Exciting news! . We invite you to the ’Dialogue on leadership roles for women in shaping academia’. The meeting is orga….

Guest editing a special issue for @ACS_Infectious. Reach out if you’d like to write something for this issue. Details 👇. Call for Papers: Infectious Diseases Research in the Global South: Treatments and Treatment Failures | ACS Infectious Diseases

Using phylogenies we trace these sequence variants in MCRs to their ancestors in bacteria, ie EptA proteins. We show how understanding evolutionary history of mobilised genes has important repercussions on making sense of differences in their activity and mutational tolerances.

We identify sequence variation at a catalytic site among MCR proteins, the primary reason for mobilised colistin resistance in gram negatives, that has consequences for level of drug resistance. We go on to identify inter-domain contacts as a source of these differences.

Many firsts for the lab in this study. First foray into mechanisms of colistin resistance, a growing and serious threat to the control of bacterial infections. First paper on structure function relationship in MCRs. First time we use #AlphaFold to make sense of mutations.