AI in Research_A User's Perspective Workshop_Why choose this workshop? Link to the second video with the workshop details: https://t.co/fG3kV1f00F The website is here: https://t.co/OXtK2c9euz

Are you curious about how bacteria defend themselves against viral attacks? Our lab at IISER Mohali explores these molecular defense pathways and their potential to inspire new biotechnological tools. Interested candidates can apply here: https://t.co/JecS3Pvqqb

Many thanks @IISERPune and @India_Alliance for generous support that allowed a study of this kind. Fantastic review process @PLOSBiology. Highly recommended! Here is the link to the paper again: https://t.co/oeQ71wxFTJ

Bottomline: Our study highlights that targeting intrinsic resistance pathways, such as efflux, can be useful to ‘resistance proof’. Yet, the gap between genetic and pharmacological inhibition is likely to be a major challenge in the application of this approach as a therapeutic.

When we try to recapitulate these findings with pharmacological inhibition using an Efflux Pump Inhibitor (EPI), E. coli pulls out another trick from its bag of adaptive strategies. Its fixes mutations in transcriptional regulators to adapt to the EPI-Antibiotic combination.

We then use laboratory evolution of trimethoprim resistance to show that impeding efflux can also significantly compromise the ability to E. coli to adapt to antibiotic. This is mechanistically explained by the dampened benefit of adaptive mutations in efflux-deficient bacteria.

We then ask how effectively impeding two well known pathways, drug efflux through AcrAB-TolC and LPS biosynthesis, reduces the MIC of resistant strains. Efflux emerges as the clear winner from our data, across antibiotic and mutant combinations.

We start by screening the Keio mutant collection for single gene knockouts that are hypersensitive to trimethoprim and chloramphenicol. Both broad spectrum antimicrobials. We discover some new targets, in addition to re-discovering many usual suspects.

Out now at @PLOSBiology we evaluate the potential for antibiotic efflux and cell wall biogenesis as targets for reversing drug resistance and preventing its de novo evolution in Escherichia coli. Link below: https://t.co/oeQ71wxFTJ

APPLICATIONS are NOW OPEN Jan 2026 PhD intake at the Department of Biology @IISERPune Submission Deadline: 5pm - OCT 23, 2025. For details, check the link below https://t.co/yPNngzWmxj

Tata IISc Medical School in Bangalore is currently hiring faculty. More information can be found here: https://t.co/4Uezh0nMhX. You can join the online event on July 26 to find out more. Please register here for link: https://t.co/y43A5giPy5. Please kindly share in your groups.

New research in #GENETICS from @NMatange and colleagues showcases canonical and non-canonical strategies employed by #Ecoli to survive in low levels of spectinomycin that facilitate genetic adaptation for acquiring antibiotic resistance. Read more: https://t.co/8PlHyIekUU

Not all persister bacteria are dormant! 🦠 Single-cell imaging of over a million #EColi shows that many antibiotic-resistant persisters were actively growing before drug exposure. Persistence appears to be more dynamic than once thought. https://t.co/KiXy38xOAY

Thanks to @GeneticsGSA for excellent and rigorous reviews. Thanks to the @India_Alliance for generous funding.

Digging deeper led us to identify some interesting links between proton/PMF homeostasis and antibiotic susceptibility. Kudos to Chinmaya @imcjena16 and his trusty accomplice @DeolankarSoham this one was no less than a Holmes mystery!

Chinmaya exposed E.coli to low levels of an environmentally relevant antibiotic, Spectinomycin. He asked if resistance is the only kind of adaptation possible. Turns out there are mutations that allow low drug specific adaptations but don’t alter antibiotic MIC values.

This is indeed a serious concern. Please intervene @IndiaDST @DrJitendraSingh @PMOIndia

Special Call for PhD intake in Biology @IISERPune This is for Dec 2024 CSIR-UGC-NET qualified candidates, whose results were recently announced. Check out details on the IISER website. The flyer lists labs taking students. Look up labs, too. Kindly RT. https://t.co/yPNngzVOHL

We have 2-3 Ph.D. positions in https://t.co/izg7N6O9BD @IISERMohali. Students interested in understanding how bacteria sense & adapt to nutrient availability & stress conditions, please apply. Deadline: April 10

1/ The Trump administration is throwing science and scientists into turmoil – how should the scientific community respond? Read the latest opinion pieces below from our new collection: ‘Science Under Threat in the US’ https://t.co/wdsKkfKj4s