📢 New preprint!! Combining transcriptomics, genetic engineering and CRISPRi silencing, we unveiled a plasmid-chromosome transcriptional crosstalk in multidrug resistant clinical enterobacteria - 1/10 🧵👇.

🔔 Check out our new opinion article published at @TrendsMicrobiol!!.

What a way to end the year, with our latest paper finally out in @NatureComms!! 🎉🍾.

RT @jaegc: Finally out after peer review. The first SuperIntegron-less Vibrio cholerae. and it is virtually identical to the WT! https://….

RT @LabMizrahi: How do plasmids interact with their bacterial hosts? 🧬🦠 .New research from @AlvSanMillan & @LauraToribioC uncovers fascinat….

In summary, we show that a pOXA-48-encoded regulator mediates a plasmid-chromosome transcriptional crosstalk, resulting in a growth benefit for the plasmid-carrying bacterium ➡️ This could promote plasmid maintenance and transmission in clinical strains.

Finally, by expressing the pOXA-48-encoded lysR in trans and by silencing the pfp-ifp operon using CRISPRi technology, we demonstrated that the overexpression of the pfp-ifp operon is beneficial for pOXA-48-carrying Klebsiella pneumoniae - 9/10 (10/10 👇)

Backed up with functional annotations of the PFP and IFP proteins and our transcriptomic and experimental results, we hypothesized that the overexpression of the pfp-ifp operon in the presence of pOXA-48 could lead to an increased iron uptake - 8/10

We also performed transcriptomic analyses of strains carrying the lysR deletion mutant of pOXA-48, and demonstrated that the LysR-mediated transcriptional crosstalk is specific to the pfp-ifp operon genes - 7/10

pOXA-48 encodes a LysR regulator that is highly similar to the LysR that controls the pfp-ifp operon. Through genetically engineering a pOXA-48 lysR deletion mutant, we demonstrated that the pOXA-48-encoded LysR is responsible for the overexpression of the pfp-ifp operon - 6/10

Remarkably, pOXA-48 induced the overexpression of a small chromosomal operon (formed by the pfp and ifp genes) in Klebsiella spp. and Citrobacter freundii. Interestingly, the pfp-ifp operon, along with its lysR regulator, shows signs of being horizontally mobilizable - 5/10

We described that pOXA-48 produces strain-specific responses on its hosts, being metabolism, transport and motility the most affected functions - 4/10

We analyzed the transcriptional response of 11 MDR clinical enterobacteria to carriage of the carbapenem-resistance plasmid pOXA-48. First, we observed that the most expressed plasmid gene was the carbapenemase blaOXA-48 - 3/10

Led with the amazing @AliciaPCV and @Lhisteria in @AlvSanMillan's lab, in close collaboration with @amazeld and @epcrocha. Thanks to all authors!! @Cris_Herencias, @AidaAdelValle, @SastreDJorge and Susana Quesada 🙌 - 2/10.

RT @ASantosLopez: Another great retreat at my grandfather's farm! This year with @asanchez_lab @AlvSanMillan @Jero_RB @TMcoque @jaegc and @….

RT @jaegc: And its out! .New paper for #integron aficionados. We found gene-less cassettes in superintegrons that express/silence other ca….

Our new study is out!!! Many thanks to all the authors! 👇👇👇.

RT @Jero_RB: 🚨 Preprint alert 🚨 . The first work from my lab where we expose the Achilles’ heel of beta-lactamase-producing enterobacteria….

RT @Cris_Herencias: Our new preprint on collateral sensitivity in E. coli is now available! We combined classic experimental approaches wit….

RT @jaegc: Check out our last work with @CelineLoot, @amazeld, @AlvSanMillan and @SkovgaardOle We have deleted the Superintegron from V. c….