Delighted to share Ben Patty's thesis research which represents an incredible amount of work, where Ben performed all the experiments and analysis presented in this massive body of work: Ben is looking for a position - find him on LinkedIn!.

RT @LucasFarnung: 🧬🎉 Our new chromatin remodeling study is out: We reveal three states of human CHD1 and identify a novel "anchor element"….

RT @maxplanckpress: Max Planck President Patrick Cramer @mpgpresident has written an open letter to the President of @Harvard, expressing h….

RT @LucasFarnung: This support is highly appreciated! From personal experience: Transatlantic cooperation is a pillar of the scientific ent….

RT @steingart: NSF GRFP #'s cut in half year-on-year . 2022: 2193 awards, 1,377 Honorable Mentions .2023: 2555 awards, 801 Honorable Me….

RT @ScienceMagazine: The U.S. National Institutes of Health’s recent decision to impose a 15% cap on facilities and administrative cost rei….

RT @cshperspectives: Big news: we are setting up a new non-profit organization to run bioRxiv and medRxiv. It's called openRxiv [no it's n….

RT @OdedRechavi: BioRxiv is the most important thing that happened in science publishing.

RT @labonnelab: On Valentine’s day, 15 Assistant Professors at NIH who had started their research labs in the last 2 years were fired. Thei….

RT @TanentzapfLab: 1. I feel like this is such obvious advice that it's taken for granted & so no one says this to young scientists but REA….

RT @LucasFarnung: In our SETD2 paper, we visualize a novel nucleosome transfer intermediate during txn. It is the earliest intermediate vis….

We are happy these studies provide new insight into coupling of nucleosome remodeling to transcription elongation. Sarah will be defending her PhD next week!.

Excited by these findings we made some mutants in mES cells to ask if disrupting this interaction resulted in similar changes to CHD distribution and nucleosome positions. Perhaps due to complexity or resolution, we don't observe these changes.

Is this mechanism for Chd1 distribution conserved in mammals? To test these we asked if murine CHD1 and CHD2 (close homolog) interact with murine RTF1 using yeast two hybrid, and yes, they do! And in fact, this is the conserved LALA box (LLSLA in mammals) and CHD1/2 CHCT

This also results in a concordant 5' shift in resident histone posttranlational modifications

This disrupted interaction and 5' Chd1 shift then leads to a 5' shift of nucleosomes, subnucleosomes, dinucleosomes, and hexasome-nucleosome (OLDN) complexes.

Using precise mutants that disrupt this interaction, Sarah found 5' shift in Chd1 localization across genes, with modest effects on RNAPII and Rtf1 occupancy

Following up from earlier studies from the Arndt lab, Sarah narrowed the interaction between Rtf1 and Chd1 to a region in the N term of Rtf1 we termed the LALA box and the Chd1 CHCT domain. Notably yeast Chd1 chromodomains do not bind K4me3 like mammalian Chd1 chromodomains

Happy to share the latest preprint which is a close collaboration with @ArndtLab with a shared PhD student, Sarah Tripplehorn. Here we show a direct interaction between Chd1 and Paf1C subunit Rtf1 required for nucleosome positioning across genes:

RT @FNucleosome: 🔔🎄Ho Ho Ho🎅🤶 .Our next #FragileNucleosome seminar is only 2 days away! We are excited to host Ryan Boileau ( @bffswithbiol….

RT @burhanco2: Immensely proud to host the Human Anatomy and Physiology Society (HAPS) Annual Conference in Pittsburgh. Dr. Natasha Baker f….