How, what, and where of environmental DNA #eDNA 🤔 Happy to share our newest publication exploring how volume of water processed and the pore size of the filter impacts the diversity of plant and animal communities captured via metabarcoding. 1/n

We kicked off this semester's #SMEE with two great talks by @DNAsaur_ and @phagemuffin. @usys_ethzh

In this study, researchers demonstrate that #diatom DNA #metabarcoding can provide consistent freshwater biomonitoring results across labs, emphasising the need for standardised protocols. 🔗 https://t.co/HGFjqMAo7h

Join our session this spring at #EGU25! You have 48 hours to pull together an abstract about your latest research bringing #biology in #hydrology with guest speaker @DNAsaur_ . Tell us about your work with #eDNA #Carbon and #diatoms for hydrological process understanding!

How, What, and Where You Sample Environmental DNA Affects Diversity Estimates and Species Detection #eDNA #environmentalDNA https://t.co/2VJuUwqOXP

See more detailed methods and a lot more analyses in the publication openly accessed via this link https://t.co/t69KaxSfVu 8/n

This is my first metabarcoding study that was conceptualized back in 2021, with a small idea and fantastic collaborators. Thanks to Florida @MaggieEHunter and Montana @gordon_luikart groups ! Big thanks to @alpineedna for the mentorship and support over the years! 7/n

Interestingly, these patters were different for ITS plant marker and COI metazoan marker! How does the source organisms impact spatial and particle size distribution of their eDNA? Can we optimize our capture methods depending on the target group? More work to be done! (6/n)

Not just the number but also the type of taxa differ. Beta diversity plots show samples cluster by location (as expected) but also by capture method ! Your choice of methods can dramatically impact your eDNA results ! (5/n)

However, the metabarcoding results show more unintuitive patterns. Although the grab samples capture 100 times less DNA, the DNA it does capture represents significantly greater alpha diversity than that captured in tow nets. These results were consistent across all sites. 4/n

We conducted the experiment in three different locations with varying water matrices: Switzerland, Montana, and Florida and found surprising consistent results ! First, we see that the tow nets (blue) captured ~100 times for DNA than grab sample filters (red). 3/n

So here we tested whether increasing volume of water processed, indeed improved species diversity detection metabarcoding. We compared two extremes of volume-pore size combinations 1) 1L via 0.45 um filter and 2) 3500-7000 L filtered via 64 um tow net.

The paper starts with a review of 300+ studies showing water volume depends on filter pore size. Thus, it may be intuitive to use larger pores to process more water and capture patchily distributed eDNA diversity. 2/n

Open PhD position in the eRNAmaris project at @AAEuibk (University of Innsbruck, Austria)! 🐟🧬🌊The project focuses on developing environmental RNA-based methodologies for assessing fish stocks in marine ecosystems. More information: https://t.co/Xz659iw18U

The 10.1016 / @sciencedirect is taking terrible decisions. It seems there is no one at @ElsevierConnect in charge of integrity issues anymore.

In unserer neuen Globe Magazine Reportage erkunden wir die Welt der #DNA, in der alle Lebewesen, vom riesigen Blauwal bis zur winzigsten Mikrobe, Spuren hinterlassen, die den Forschenden helfen, die #Biodiversität zu bestimmen. 🧬 https://t.co/o1JZcNGeUv @DNAsaur_ @SimplexDNA

«Zerstören wir die Biodiversität, zerstören wir unsere Zukunft», sagt Kristy Deiner, Professorin für Umwelt-DNA am @usys_ethzh in der NZZ-Beilage «Sustainable Switzerland» vom 15.09.2024. 🌱💚 #Biodiversität #Abstimmung #eDNA @alpineedna https://t.co/lFKQUr89e7

Another new snake species! A new long-snouted vine snake from India https://t.co/iGzWdzO8yy @HarshilPatel89 #herpetology #newspecies #India #Bihar #Assam

Trending in #WaterResources: https://t.co/ual4VxqHsE 1) Glyphosate contamination in European rivers not from herbicide application? (@WaterRes_J) 2) Quantifying urban flood extent using satellite imagery 3) A new flow path: eDNA connecting hydrology & biology 4a) Nitrogen

A new flow path: eDNA connecting hydrology and biology #eDNA #environmentalDNA https://t.co/T8QH0DufgB