Tomasz Góral
@t_goral
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Cryo-EM specialist, Microscopist, Biologist, Cryomicroscopy and Electron Diffraction Core Facilty, @UniWarszawski, #cryoem, #microED
Centre of New Technologies UW
Joined July 2022
We are one of the two cryoEM Core Facilities in Poland equipped with a 200kV Glacios microscope capable of three major cryoEM modalities: #singleparticle, #cryoET, #microED. We are open access to both academia and industry.
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Another fantastic results published in #JACS. This work shows a great potential of #microED for studying mineralogical and geological samples. ED data were acquired on our Glacios at CeNT UW.
pubs.acs.org
In minerals and inorganic compounds, strong hydrogen bonding can lead to the formation of complex ionic species such as the H3O2– bihydroxide anion and Zundel cation H5O2+. We studied [NaCu2(SO4)2·...
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Here it comes! A long-awaited paper led by @MatyldaIzert and @m_gorna is now out. It features multi-mode approach combining different techniques including our Glacios-made 2.45A cryo-EM stucture of GroTAC peptide interacting with GroEL protein (PDB ID: 8S32).
Fantastic work by @MatyldaIzert pioneering Targeted Protein Degradation in Escherichia coli using a PROTAC-like approach!🎉 .Could the future of antibiotics be #TPD?💊🧫.Thanks to FIRST TEAM @FNP_org_pl funding.
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The latest paper in #JACS features high resolution structures of flexible MOFs that were obtained from nanocrystals and measured on our Glacios using #microED
pubs.acs.org
Flexible metal–organic frameworks (MOFs) are porous materials exhibiting spatiotemporal responses to environmental changes, which significantly affect their sorption properties and offer potential...
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RT @MariuszCzC: The article was published in JBC and is available here:.I wish to thank all the authors @IIMCB_Pola….
www.jbc.org
In archaea and metazoa, tRNA exons are ligated by the RNA ligases RtcB and RTCB, respectively. The metazoan RTCB forms a stable complex with four additional subunits, DDX1, FAM98B, CGI99, and ASHWIN....
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Combining cryo-EM results with molecular dynamics simulations can lead to accurate determining the positioning of memebrane-active peptides within a lipid bilayer. Check out this great paper published in #JACS with data acquired on our Glacios microscope.
pubs.acs.org
We propose an approach for determining the positioning of membrane-active peptides within a lipid bilayer. It is based on a combination of cryogenic electron microscopy (cryo-EM) with molecular...
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Please have a look at this beautiful work of one of our past users dr Piotr Szwedziak who demonstrated that cryo-ET is doable on 200kV microscopes even without energy filter. All data shown here were collected on our Glacios.
www.biorxiv.org
Electron cryotomography is a powerful imaging technique allowing for studying functional cellular modules in their native environment with macromolecular resolution. However, it requires access to...
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A brand new FEG emitter on our Glacios allowed for a significantly reduced exposure times for a given dose which for the first time resulted in an acquisition rate exceeding 300 movies/hr! (Falcon3 detector in linear mode with AFIS enabled)
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After ~5.5 years our Glacios is getting a new heart. We are replacing the X-FEG emitter so we continue providing with high quality results in the future.
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All welcome to join our seminar summarising 5 years of cryo-EM Core Facility at CeNT UW.
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A new micro-ED paper from our lab has just been released highlighting the potential of electron diffraction in plant biomineralisation. #microed, #Glacios, #cryoEM. #mdpimolecules przez @Molecules_MDPI.
www.mdpi.com
Plants are able to produce various types of crystals through metabolic processes, serving functions ranging from herbivore deterrence to photosynthetic efficiency. However, the structural analysis of...
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I'm glad to announce that we've received a nearly 1 mln PLN grant from the Polish Ministry of Science to maintain our Glacios microscope at CeNT UW. We warmly invite all interested users to take advantage of the services offered by our laboratory.
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A great work on Hirshfeld atom refinement and dynamical refinement of hexagonal ice structure from ED data has just been published in #IUCrJ. All measurements were done on our Glacios microscope.
journals.iucr.org
Kinematical Hirshfeld atom refinement has been applied to electron diffraction data for the first time, but the effect of using an aspherical atom model is overshadowed by dynamical scattering...
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RT @m_gorna: Another fantastic collaboration with @MissAgataStar 😍We can finally play with some ribosomes too! 🤗.Frozen yesterday by @KJBan….
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RT @ActaCrystC: TAAM refinement on high-resolution ED/MicroED data @ActaCrystC @IUCr @ChemistryUW @UniWarszawski @N….
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Yet another paper on high resolution microED/3DED data from @UniWarszawski produced on our Glacios microscope
journals.iucr.org
With the currently avalaible 3D ED data it is possible to observe details of electrostatic potential deformations due to chemical bonding in organic crystals. The deformation signal is strong enough...
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RT @m_gorna: A pioneering adventure by @MatyldaIzert - the first way to do protein knockdown in E.coli! Of unmodified, degronless targets😍U….
www.biorxiv.org
New, universal tools for targeted protein degradation in bacteria can help to accelerate protein function studies and antimicrobial research. We have created a new method for degrading bacterial...
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