RT @BenjaminKroeger: It's done! Proud to be part of the @AusFly2024 organising team. Cheers @DrTeresaBonello @schmanning and Alistair Senio….

RT @DrTeresaBonello: Wonderful couple of days in picturesque Marysville for the Australian Fly Meeting @AusFly2024 .Thanks to the amazing t….

RT @ECBrooks92: Very grateful for the opportunity to speak about @HarveyHippoLab research at @AusFly2024! It's been a great day one and I'm….

RT @KatMitchell_Sci: Very excited to share our #preprint in @biorxivpreprint in which we found that the transcription factors of JNK and Hi….

RT @HarveyHippoLab: Congratulations almost Dr ⁦@HarmanjeetS1⁩ on a wonderful final PhD seminar - the first for the ⁦@MonashBDI⁩ chapter of….

Thanks so much to all the co-authors and members of @HarveyHippoLab for their contributions, especially @BenjaminKroeger and our collaborators @Liz_Hinde_ for showing us the power of RICS to visualise the goings-on within the nucleus. 8/8.

These results indicate that Scalloped and Yorkie alter each other’s behaviour within the nucleus and give insights into how the Hippo pathway regulates gene expression. 7/8.

We wondered whether transcriptional coregulators might change Scalloped’s behaviour at these sites, as a mechanism to alter gene expression. We found using FRAP that the Yorkie activator increased Scalloped’s binding time, whilst repressors decreased long binding events. 6/8.

By imaging single Scalloped molecules, we found their binding duration was extended at these sites relative to elsewhere in the nucleus. 5/8.

We noticed regions in the genome where the transcription factor Scalloped and its coactivator Yorkie were both highly enriched, which were high in markers of transcriptionally active chromatin. 4/8

We saw that Yorkie explored the nuclear environment more rapidly than Scalloped, but Yorkie’s behaviour was highly dependent on the levels of Scalloped within the nucleus. 3/8.

We thought the easiest way to investigate these processes was to watch them, so imaged the giant nuclei in salivary glands of D. melanogaster larvae. This enabled visualisation of transcription factor movement throughout the nucleus as well as DNA binding behaviour. 2/8

Excited to finally share our work in @Dev_Cell describing how Hippo pathway transcription regulators navigate the nuclear environment and interact with the genome to control gene expression in live tissues. 🧵1/8

RT @ECBrooks92: Delighted to have this work from my PhD published!.Using the amazing genetics and imaging capabilities of #Drosophila, we s….

RT @BenjaminKroeger: 🍾Our paper is officially out today in @Dev_Cell. Delighted to share it with y'all: Thanks aga….

RT @MonashBDI: Monash researchers discover key to epithelial cell growth. Led by @HarveyHippoLab, first author @BenjaminKroeger, published….

RT @BenjaminKroeger: Excited to share our #preprint in which we uncover a new subcellular site of Hippo pathway signalling with links to ti….

RT @apdrc6: Lots of excited conference attendees board the boat out to the reef 🪸 #APDRC6

RT @BenjaminKroeger: With the Monash Uni crew exploring sunny Cairns before #APDRC6 starts tonight

RT @apdrc6: We look forward to welcoming attendees at the conference venue for #APDRC6 in sunny and lush Cairns, Australia. The conference….