I am excited to share that our preprint “Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila” is now available on BioRxiv. A thread🧵

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RT @eLife: How individual insulin-producing cells in the fruit fly brain work together to produce a concerted response to metabolic demands….

RT @TinaHeld4: I'm over the moon that our paper is now available as reviewed preprint on @eLife: You can find a gr….

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Thrilled to share this story at #icn2024berlin @neuroethology Catch me at Poster PS1.087 to dive into our latest findings published in @eLife! . "Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila"

RT @jan_ache: Thrilled that this story is now out in @eLife as a reviewed preprint:

RT @FenselauHenning: Our new paper on NPY-mediated synaptic plasticity is out!!! Many congrats to @DodtS who led the project at @MPI_Metabo….

10/n Grateful to all the contributing authors who made this possible @fathimakessiah, @dr_feffone, Till Bockemühl, my supervisor and mentor @jan_ache for his immense support and guidance at each and every step. Finally, a big thanks to @dfg_public, for funding our work.

9/n In conclusion, our study delves into complex activity dynamics of IPCs and DH44Ns, unveiling intricate connections between neuroendocrine signaling, nutrient sensing, and behavior in Drosophila.

8/n These findings suggest that feeding-promoting and satiety-inducing pathways form inhibitory connections in the CNS as a part of the neuronal mechanisms to ensure reciprocal inhibition of satiety and hunger states.

7/n Finally, we hypothesized that DH44Ns could have opposite effects on behavior since a subset of these neurons inhibit IPCs. Activation of DH44Ns indeed led to a strong increase in locomotor activity followed by proboscis extensions.

6/n Next, to investigate potential connectivity between DH44Ns and IPCs, we optogenetically activated DH44Ns while patching IPCs. These experiments showed that DH44Ns outside PI strongly inhibit IPCs , highlighting complex neuroendocrine interactions.

5/n Unlike IPCs, the spike rates of DH44 neurons didn't significantly differ between starved and fed flies. However, the inter-spike-intervals distribution were shifted to longer intervals during starvation. This may contribute to increased glucose sensitivity in starved flies.

4/n As experimental controls, we also recorded from DH44 neurons in the pars intercerebralis (PI) which have been previously shown to be activated by glucose. We confirmed that these neurons are indeed sensitive to glucose perfusion in starved flies – in contrast to the IPCs.

3/n Surprisingly, while IPCs were sensitive to glucose ingestion, they did not respond to glucose perfusion. This is reminiscent of the 'incretin effect' in mammals, where insulin secretion is significantly higher when glucose is ingested orally compared to intravenous delivery.

2/n Next, we investigated how the nutritional state and IPC activity modulate locomotor activity. Activation of IPCs reduced starvation-induced hyperactivity, underscoring their role in foraging behavior. This effect was small compared to other modulatory neurons, but significant

1/n We dove into the in vivo dynamics of Insulin-Producing Cells (IPCs), using electrophysiology to uncover how nutritional state affects their activity. As expected, we found that the nutritional state strongly modulates IPC activity, especially after glucose ingestion.