5/ In this preprint, we show that FLIPR is a powerful technology that will allow the community access to absolute fast and slow signal measurement in vivo. Thank you to my mentors @blsabatini Roger Adan, coauthors and Sabatini lab members.

4/ We discovered differences in tonic dopamine levels at baseline and differential phasic responses to appetitive and aversive stimuli between brain regions. We also discovered differential and dynamic changes in tonic dopamine in response to aversive stimuli.

3/ To highlight the capabilities of FLIPR, and in collaboration with @LinTianPhD , we exposed freely moving animals to appetitive and aversive stimuli, while measuring phasic and tonic dopamine in two brain regions (the tail of striatum and nucleus accumbens).

2/ FLIPR allows for accurate, high speed (10-1000 Hz) and high precision (<5ps error) fluorescent lifetime measurement and is stable across time and photon rates. FLIPR is 100-1000x faster, more precise and is more stable across photon rates, compared to other FLIP systems.

1/ We created fluorescence lifetime photometry at high temporal resolution (FLIPR) by developing a frequency domain fluorescent analog processor and combining it with a simplified fluorescence lifetime photometry optical system for lifetime measurement through a fiber implant.

If you are interested how to measure absolute neurotransmitter release or neuronal signals in vivo over long time-scales and at high speed, please check out our new pre-print, where we use FLIPR to measure phasic and tonic dopamine in freely moving mice.

RT @blsabatini: This is something we are very excited about. @lodder_bart led this development project collaborating with @LinTianPhD lab.….

RT @RichieHakim: Hello calcium imaging twitter, I have a thread for you. We made a self-supervised network that is great at ROI classificat….

RT @VidaConference: ViDA is excited to host @blsabatini for a keynote lecture on "Dopaminergic control of cellular state and action selecti….

RT @blsabatini: I am retweeting now because it links to the explanation of the findings in the just-published. Thir….

RT @_JaeeonLee_: Extremely comprehensive work, with lots of parallel with our recent work. Just highlights how much….

RT @blsabatini: 1/n We've posted a new study led by @sarahmelzer86 in collaboration with @LinTianLab. Bombesin-like peptide recruits disin….

RT @biorxiv_neursci: Serotonin neurons modulate learning rate through uncertainty #biorxiv_neursci.

RT @blsabatini: Are you or do you know an experience mouse colony manager who needs a job? It can be part time. Find our ad for HHMI funded….

RT @blsabatini: Very cool. Falls under the heading of the best ideas are often obvious in retrospect.

RT @georg98keller: Mesmerizing cortical activity - we saw this earlier today (i.e. we have no clue yet what it is - aside from "probably a….

RT @gottapatchemall: Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.https:/….

RT @biorxiv_neursci: Dopamine neuron synaptic connectivity defines physiological striatal domains #biorxiv_neursci.

RT @Ulrike_Boehm: .@HHMIJanelia’s Optical Interest Group is currently partnering the @embl in a monthly seminar series. The presentations w….

RT @dacolon: This is a question that I frequently get from students. I start by labeling: Graduate school is as much about making discoveri….