New preprint from collaboration with @SN_Lab - 2p imaging of psilocybin's effects on neurovascular coupling 🔬. We found that psilocybin prolongs the neurovascular response, independent of neural activity. This affects how we should interpret fMRI BOLD studies of #psychedelics‼️.

RT @AllenInstitute: Holy Soma Batman!. You never know what you may find when you cruise our MICrONS Explorer, such as a blood vessel pierci….

RT @WackerLab: A take on the current state of biased signaling in psychedelic action and psychedelic drug discovery by John McCorvy and I:….

@OneMindOrg This was a team effort spearheaded by Quan Jiang. With help from collaborators at UC Irvine, CUHK 🇭🇰, and @AllenInstitute, and support from @OneMindOrg and @NIMHgov. 12/12

@OneMindOrg 2) We demonstrate that neural activity modulation may be a promising approach to sculpt the psychedelic-evoked neural plasticity. 11/12

@OneMindOrg Two conclusions:. 1) Psilocybin induces network-specific plasticity. There was strengthened routing of inputs from RSP (mouse homolog of default mode network) and perceptual regions to subcortical targets. Inputs that are part of cortico-cortical loops were weakened. 10/12

@OneMindOrg If neural activity influences whether a region undergoes remodeling after psilocybin treatment, can we causally manipulate this plasticity?. We show this is possible - chemogenetic silencing of RSP disrupted psilocybin’s ability to strengthen those inputs. 9/12

@OneMindOrg To investigate this possibility, we focused on one presynaptic region – the retrosplenial cortex (RSP). Neuropixels recording confirms that indeed RSP neurons that project to frontal cortex fire more in response to psilocybin. 8/12

@OneMindOrg What drives this pattern of psilocybin-induced circuit remodeling?. One clue came from whole-brain c-Fos mapping, which correlated well with the input changes, suggesting neural activity in the presynaptic regions may play a role. 7/12

@OneMindOrg We also traced from IT pyramidal cells, which interestingly have opposing changes in input fraction after psilocybin. What were strengthened for PT tend to be weakened for IT, and vice versa. 6/12

@OneMindOrg The regions were not random though, increased inputs came from regions belonging to specific cortical networks, while decreases were inputs originating from other networks. 5/12

@OneMindOrg Our hypothesis was that psilocybin may boost inputs from one or few regions. We would identify those key regions…. Surprisingly, tracing from PT pyramidal cells, our results show widespread changes with inputs from many regions altered after psilocybin administration. 4/12

@OneMindOrg We would inject psilocybin or saline, then used monosynaptic rabies viruses to trace the input cells to the frontal cortical pyramidal neurons. The whole-brain images were beautiful! . 🔴starter cells. 🟢 input cells. #FluorescenceFriday. 3/12

The idea was to find circuits that are altered by #psychedelics, going beyond simply an increase of dendritic spines. In the grant proposal, this figure was exactly how I pitched to @OneMindOrg, who believed in the vision and provided the critical support for the project. 2/12

New preprint + thread 🧵. #Psychedelics induce the formation of new synapses, but where do they connect?. Our rabies tracing study reveals that #psilocybin shifts connectivity across specific cortical networks. 1/12

RT @Mahathi_VR: It was an immense pleasure to have gotten an opportunity to give an oral and a poster presentation of our work at the #GRCP….

RT @JHPsychedelics: Reminiscing on all the great presentations and conversations we had at the Gordon Research Conference @GordonConf. Our….

RT @VeronicaChelu: Had a fantastic time at the Neurobiology of Psychedelics GRC. Only a week yet feels like it could have been months. New….

A great writeup of the science at #GRCPsychedelics by @JasterAlaina 🔥.

RT @TheBorisLab: Better late than never: The recent #GRCPsychedelics was really marked for me by two key moments: my lab delegation (past,….