Kaisa Pakari
@kaisa_pakari
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PhD candidate @WittbrodtLab & @_ThomThum_ at @COSHeidelberg MSc Molecular Biotechnology @UniHeidelberg
Heidelberg
Joined May 2019
Get ready for enzyme limbo & check out our new preprint: We knockdown Pmm2-GFP with a mRNA-delivered degron system in an inducible manner in medaka. Link: https://t.co/8xZRG05Ddq Thanks to @_ThomThum_, @WittbrodtLab & colleagues #greennomore #medaka #CRISPR
biorxiv.org
Creating hypomorphic mutations are crucial to study gene function in vivo , especially when null mutations result in (embryonic) lethality. This is especially the case for enzymes involved in...
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A mind-blowing paper has come out today in @Nature In 2016, JC Venter Institute scientists trimmed a bacterial genome to its barest minimum required for life to synthesize what they called a "minimal genome" ( https://t.co/CNDeaZUmYd). Today, a group of scientists from
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CRISPR-Fortschritte - Schnitt für Schnitt zu neuen Möglichkeiten: unser Review über sukzessive, kleine Fortschritte, die gezieltes Genomeditieren mittels CRISPR & BaseEditoren effizienter gemacht haben https://t.co/xcj5Qn3uXh ✂️ mit @kaisa_pakari & @WittbrodtLab im @Spektrum_BIO
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Just published @Dev_journal, Kaisa (@kaisa_pakari) presents our "inception" approach to target previously inaccessible nucleotides with base-editing at the 1st Heidelberg #CRISPR mini symposium @MKolleg today - link to paper: https://t.co/ggZkKV3uB5
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Here it is: our #inception paper @Dev_journal „De novo PAM generation to reach initially inaccessible target sites for base editing“ by @kaisa_pakari & @_ThomThum_ We use 2 guides & 2 editors to edit distant nucleotides with NGG base-editors https://t.co/E4Qm7dCpRu
#medaka #DNA
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What a week! Poster presentation at #JGMglyco32 and giving my first talk at #GfE2022 on studying glycosylation disorders in #medaka + targeting out-of-reach nucleotides using base editors and "inception" -> https://t.co/XPu2b7ubyF
#baseediting #glycosylation #WittbrodtGang
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At #GfE2022, Kaisa Pakari @kaisa_pakari from @WittbrodtLab @_ThomThum_ presents recently developed genome editing wizardry, enabling her to engineer #medaka mutants of human diseases that would be out of reach for regular #CRISPR approaches. Check out https://t.co/rFITWAkEcI
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Let's switch gears and look at glycosylation diseases, with Kaisa Pakari and medaka fish.
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Want to base edit and no PAM in reach? Just create one! "Inception" is a one-shot event based on a canonical target site to create a de novo PAM and a 2nd editing upstream, relaxing steric dependencies. #CRISPR #baseedit with @kaisa_pakari, @WittbrodtLab
https://t.co/U9foGqruAW
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Richard Wombacher and colleagues (@LabWombacher) report a new chemical inducer of proximity based on ABA receptor dimerization by the agrochemical manipropamid. Open access:
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I am really excited to share that our work on the translational #medaka model for a rare disorder of glycosylation made it onto the cover of the most recent @Dev_journal issue. #CDG 🐟
Our 11th issue of 2021 is now complete! The cover shows the vasculature of a medaka embryo, from @1higgsbozonu @_ThomThum_
@WittbrodtLab & colleagues https://t.co/WvUYin9zF8
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Very happy I got the chance to contribute to this exciting story on a patient-based medaka alg2 mutant as a model for hypo-N-glycosylation in @Dev_journal !
1/4 Excited to share our new paper from @WittbrodtLab published in @Dev_journal. We used a CDG-patient-based targeted mutagenesis (#CRISPR) in #medaka fish to establish a stable and fully rescuable model with an impaired mannosyltransferase Alg2.
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