How diverse is protein localization to human primary cilia? Check our study revealing incredible cell-type and single-cell diversity: .3 year effort of studying 1935 proteins with imaging - 654 ciliary proteins pictured!.Tweetorial by @Prof_Lundberg & ⬇️.

Excited to share our first preprint from our project on an AI-assistant for microscopy! 🎉 This was such a fun collaboration on creating a resource of image-question pairs - not only useful for ML development but also for learning & testing biological image interpretation skills!.

Interesting for #Cilia researchers: SubCell discovered three types of characteristic cellular microtubule localization patterns in (non-ciliated) U2OS cells that relate to cilia proteins. You find a short paragraph on this observation at the end of the preprint 😃.

Have a look at our latest advance in exploring (sub)cellular architecture from fluorescence microscopy images ⬇️.

RT @naturemethods: The wait is over!! We are thrilled to announce that we have chosen Spatial Proteomics as 2024’s Method of the Year! 🥳. F….

Thank you for organizing this great event series @Mill_lab and bringing the cilia world together! And thank you everyone for all the feedback, questions, and discussions!.

Big thank you to the incredible @Prof_Lundberg lab team behind this study, and our excellent collaborators and @karolinskainst Anna and Eini!.

. or from tonight on, you will find the images for the 654 ciliary proteins and more online in the v24 of the @ProteinAtlas:

Check out more interesting new ciliary proteins in the preprint, such as metabolic enzymes, a new cilia marker protein (CFAP300), or CREB3 in cilia, for which we found a coding genetic variant related to blindness and skeletal changes!.

Our atlas portrays the cilium as a dynamic sensory organelle, suggesting that individual cells can fine-tune their cilium - both in terms of receptors and signaling components - to sense and integrate diverse signals, computing a response to relay to the cell body.

Stunning combination of ciliary diversity and multilocalization for CKAP2.

70% of cilia proteins cell-type-specifically localize to primary cilia, 78% show variation across cilia in the same cell type. In 90% of the images they localize elsewhere in the cell - at the same time (multilocalization). A great example: AKT3.

We reveal many signaling proteins in cilia, also of downstream signaling cascades, portraying the cilium not only as an antenna but also for downstream cellular computations.

Manually annotating all generated data, we revealed 654 proteins at cilia with their subciliary localization pattern – distinguishing primary cilium, tip, transition zone, and basal body localizations.

Over 2.5 years, we had a microscope running day and night - recording 17,092 confocal 3D image stacks of antibody stainings for 1,935 proteins in three diverse human cell lines, containing (according to CiliaQ) in total 463,368 cells with 218,412 cilia.

RT @friedrich_group: "The Biophysics of Motile Cilia" Heraeus.@seminar_cilia: Application deadline for contributed talks Dec 1st! Topics: #….

Or come to my talk on "Spatiotemporal dissection of protein localization to human primary cilia" at #HUPO2024 in the ECR session on "Innovative approaches to study protein function" on Thursday!.

Cilia and Flagella enthusiasts, stay tuned for tomorrow when the cells in the @ProteinAtlas will grow cilia and flagella. If you are at #HUPO2024, come to the plenary session tomorrow at 9.15 am by @Prof_Lundberg and the release of version v24 (with cilia) at 6.40 pm CET!

Looking forward to speaking about heterogeneity of primary cilia today at the DGZ Focus Workshop on Cilia and Centrosomes:.

2. The following call in the terminal creates an output.pdf representing input.pdf with the bookmarks from (see 1.):. gs -sDEVICE=pdfwrite -q -dBATCH -dNOPAUSE -sOutputFile=output.pdf -dPDFSETTINGS=/prepress -f input.pdf.