In an interview with HPA Director Mathias Uhlén in Sweden's leading business newspaper Dagens Industri the race to build a virtual cell and the major initiatives such as AlphaFold, the Chan Zuckerberg Initiative, Pi-HuB and Alpha Cell are discussed.

The cell cycle is fundamental for life, enabling organisms to grow, heal, reproduce, and maintain cellular function. In the Human Protein Atlas protein imaging and single cell RNAseq analysis have been used to explore the cell cycle dependent proteome.

In an article in Genome Medicine HPA related researchers have used advanced multi-omics analysis to investigate the impact of genetics and environment on the metabolic profiles of healthy adults by tracking metabolic changes over a two-year period.

ATP is essential for the folding, maturation, transport and degradation of proteins in the ER. These constant processes all rely on ATP import from the cytosol, but it is not until recently that the responsible transporter, SLC35B1, has been identified.

Spermatogonia are the stem cells of spermatogenesis and early mitotic stage progenitors of sperm cells. Using mIHC/IF proteins can be localized at different states of differentiation which show IGF2BP1 in undifferentiated sperm cells in testis.

In a correspondence in Nature Medicine the initiative PROMISE (Precision Omics Initiative Sweden) with the aim to connect research with healthcare and establish Sweden as a major player in the field of data-driven precision medicine was presented.

In a Nature article researchers from HPA and UCLA have used a machine learning approach that enables computational integration of protein localization data from IF images and AP-MS protein interaction data to create a MuSIC map of 5,100 proteins in U2-OS.

In the human proteome there are still many rather unknown but potentially interesting genes that deserve some extra attention. Here we will focus on C19orf67, a gene with low evidence but specific and consistent expression detected in the testis.

In a publication in Neuron, authored by the HPA-related researcher Jan Mulder, Stereo-seq in combination with single-nucleus RNA sequencing data have been used to create a 3-D spatial gene and cell atlas of the adult mouse brain.

Professor Mathias Uhlén was interviewed in GenomeWeb about the new Alpha Cell project, a $36.5 M funded joint effort between SciLifeLab and KTH, with the aim to map and model cellular components and processes across time and in response to perturbations.

Multiplex immunohistochemistry enables visualization of complex staining patterns in structural components of the salivary gland. Staining of CRACR2A is localized to the cytoplasm of serous acini and the apical part of the membrane of large ducts.

Images generated using CODEX technology for simultaneous immunostaining of 47 marker proteins have been used in HPA to show the distribution of a large set of reference markers across all major tissues and organs in the human body.

A Nature editorial explores how researchers use methods like high-resolution imaging, mass spectrometry, and machine learning to reveal the dynamic locations of proteins inside human cells and feature the Human Protein Atlas as a pioneering initiative.

This month the protein CFAP45, a component of the protein complexes of the axoneme of motile cilia, is highlighted among the sparse population of ciliated cells in endometrial glands using multiplex immunohistochemistry (mIHC/IF).

Proteins found in multiple subcellular locations is an interesting group of proteins that includes shuttling proteins, proteins involved in processes shared between organelles, and "moonlightning" proteins that are multilocalizing and multifunctional.

Listen to KTH professor Mathias Uhlén, founder of the Human Protein Atlas and co-founder of numerous biotech companies, when he discusses science, entrepreneurship and lots of other things with Philip Hemme in Europes leading biotech podcast.

Among the about 20,000 genes in the human proteome there are still many rather unknown but potentially interesting genes that deserve some extra attention. Here we will focus on SMIM17, a gene with little evidence and elevated expression in the brain.

In J Proteome Res HPA researchers present the current progress of HPPP and discuss how updates of proteomics methods, new technologies, and investigations of proteoforms and protein networks can impact the understanding of the circulating proteome.

The cytoskeleton-associated intermediate filament protein Nestin is shown to be localized (green) to the cytoplasm of glomerular endothelial cells (magenta) and podocytes (yellow) in kidney using multiplex immunohistochemistry (mIHC/IF) .

Sperm cells are specialized for their function in reproduction with a unique structure and unique proteins. HPA researchers now map proteins in sperm using IF and confocal microscopy to get a single-cell resolution view of the location of sperm proteins.