Gabriel M. C. Longo
@genomewizard
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PhD in Molecular Biology interested in genomics, DNA repair and gene editing. Research Fellow at HMS/MGH. Former @IMBmainz and @EMBL
Boston, MA
Joined October 2020
It’s my pleasure to share that our latest manuscript is out now in Nature Biotechnology. This has been an incredibly exciting project! We describe BreakTag, an NGS-based tool for the rapid characterization of CRISPR nucleases and off-target activity.
nature.com
Nature Biotechnology - The genome-wide landscape of Cas-induced double-strand breaks and end structures is profiled at nucleotide resolution.
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RT @NicoleGaudelli: .@SecKennedy - thank you so much for your broad support for gene editing and gene therapies as well as recognizing the….
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RT @mgdurrant: Really nice characterization of Cas9 DSBs in human gDNA, with thorough off-target profiling and modeling of DSB outcomes for….
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RT @NatureBiotech: The genome-wide landscape of Cas-induced double-strand breaks and end structures is profiled at nucleotide resolution ht….
nature.com
Nature Biotechnology - The genome-wide landscape of Cas-induced double-strand breaks and end structures is profiled at nucleotide resolution.
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RT @BKleinstiver: In @nature we describe the use of scalable #proteinengineering & #machinelearning to predict millions of bespoke CRISPR-C….
nature.com
Nature - Combined high-throughput protein engineering with machine learning to curate libraries of CRISPR genome-editing enzymes with distinct genome targeting properties is described.
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RT @Nature: A scientist who successfully treated her own breast cancer by injecting the tumour with lab-grown viruses has sparked discussio….
nature.com
Nature - Virologist Beata Halassy says self-treatment worked and was a positive experience — but researchers warn that it is not something others should try.
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RT @imbmainz: Our Roukos lab discovered that cells use type II topoisomerases to regulate the 3D folding of DNA. This is important for cont….
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RT @sfb1361: Publication alert! 📰. Great job to @RoukosVassilis and collaborators on the new publication in @MolecularCell!. Read more abou….
cell.com
Longo et al. show that TOP2s act as a fine regulator of chromosome folding at multiple scales. TOP2 actions control large-scale contacts between active, positively supercoiled genome regions and...
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RT @AkisPapantonis: The @RoukosVassilis lab led a new and detailed study of Topoisomerase II contribution to #3Dgenome folding in human cel….
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RT @RoukosVassilis: Happy to share our new work in collaboration with the @MundlosLab @StefanMundlos & @AkisPapantonis labs, published toda….
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RT @MolecularCell: Online Now: Type II topoisomerases shape multi-scale 3D chromatin folding in regions of positive supercoils https://t.co….
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Maria Stefanova, Ting Xie, and Waheba Elsayed, who co-led the study. Huge shoutout to IMB’s Genomics, Microscopy, and Bioinformatics core facilities and the contributions of all co-authors. (7/7).
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influencing chromatin interactions across various scales. This study was a collaborative effort between the Roukos Lab (@imbmainz), the Papantonis Lab (University of Göttingen), and the Mundlos Lab (MPI for Molecular Genetics). Special thanks to Sergi Sayols, (6/7).
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These overtwisted regions were enriched at the promoters of highly transcribed genes, TOP2A/B binding sites, and, intriguingly, at chromatin loop anchors. We propose that TOP2s act as fine-tuners of 3D genome organization in the interphase nucleus, (5/7).
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we devised a CUT&RUN-based method using GapR, a bacterial protein that specifically binds to positive supercoiling. Through GapRUN, we generated a high-resolution map of positive supercoiling across the human genome. (4/7).
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Many appeared as Mb-sized domains with increased interactions, suggesting TOP2s help organize chromosomes, particularly between Lamin-Associated Domains (LADs) and actively transcribed chromatin. To further understand these observations through the lens of supercoiling, (3/7).
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These enzymes, often chemotherapy targets, have known roles near chromatin loop anchors, prompting us to investigate their influence on 3D genome organization. By selectively perturbing TOP2A and/or TOP2B, we observed hundreds of differential chromatin contacts. (2/7).
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Excited to share our latest research out now in @MolecularCell. DNA supercoiling creates topological issues that affect nuclear DNA transactions, with cells using topoisomerases to resolve these constraints. (1/7).
cell.com
Longo et al. show that TOP2s act as a fine regulator of chromosome folding at multiple scales. TOP2 actions control large-scale contacts between active, positively supercoiled genome regions and...
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RT @imbmainz: Join us as a Group Leader in #ComputationalBiology or #RNA Biology 🧬. More details via Application de….
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I'm thrilled to have completed this challenging yet deeply rewarding journey. My PhD experience was absolutely amazing, and yesterday I received the summa cum laude distinction for my defense. #Done
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